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World Journal of Gastroenterology 1997年第4期3卷 30-32页

作者： WANG MaoRong, LE MeiZhao, XU JiaZhang and HE ChangLun

IM To establish a new experimental model system with human fetal hepatocytes to study the mechanisms of protective effect of silybin and polyporus umbellalus polysaccharides (PSP) on the ultrastructure of human fetal hepatocytes.METHODS The hepatocytes were obtained from the liver of human fetus from induced labor with written consent. The primarily cultured hepatocytes were pretreated with silybin and PSP and exposed to CCl4 for 4 hours. The ultrastructural changes of hepatocytes were observed under scanning electronmicroscopy (SEM) and transmission electronmicroscope (TEM). Transaminase and SOD were also assayed at the end of culture.RESULT The levels of ALT and AST were significantly decreased and the SOD level elevated in two pretreated groups as compared with that in the control group. The cellular integrity and ultrastructure of hepatocytes were well preserved in the two drugs pretreated groups while they were seriously damaged in the control group.CONCLUSION The experimental model system with human fetal hepatocytes pretreated with CCl4 could act as an effective method for studying the protective effect of drugs on human hepatocytes, and could be used for screening the medicines for treatment of hepatitis.

关键词： fetal hepatocytes experimental model silybin polyporus umbellalus polysaccharides

Monosaccharide analysis and fingerprinting identification of polysaccharides from Poria cocos and polyporus umbellatus by HPLC combined with chemometrics methods

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Chinese Herbal Medicines 2019年第4期11卷 406-411页

作者： Jie Liu Jing Zhou Qian-qian Zhang Min-hang Zhu Mo-li Hua Yun-hui Xu State Key Laboratory of New Drug and Pharmaceutical Process Shanghai Institute of Pharmaceutical IndustryChina State Institute of Pharmaceutical IndustryShanghai 201203China

Objective:Poria cocos and polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chemometrics methods has been developed for characterization and discrimination of them in this ***:The polysaccharides were extracted by decocting in water,and then completely hydrolyzed with *** in the hydrolyzates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)for HPLC *** than 20 batches of *** and *** from different regions were ***:The fingerprints of *** showed five common characteristic peaks,which were identified by comparing with the reference *** five peaks corresponded to the derivatives of mannose,ribose,glucose,galactose,and *** the same time,the fingerprints of *** showed eight common characteristic peaks,of which seven were identified as the derivatives of mannose,ribose,rhamnose,glucose,galactose,xylose,and ***,the similarity of their fingerprints was respectively calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM published by China Pharmacopoeia Committee(Version 2004 A).And the data were further processed by hierarchical cluster analysis(HCA)and principal component analysis(PCA).The similarity evaluation and HCA indicated that there were no significant difference in *** or *** samples from different geographical regions,but PCA was performed to characterize the difference in monosaccharide constituents between *** and ***,and linear discriminant analysis(LDA)showed the overall correct classification rate was 100%.Conclusion:The fingerprint analysis method of monosaccharide composition of water-soluble polysaccharides can distinguish *** and ***,and can be applied for the authentication or quality control for *** and ***.

关键词： fingerprint hierarchical cluster analysis(HCA) linear discriminant analysis(LDA) polyporus umbellatus(Pers.)Fries polysaccharides Poria cocos(Schw.)Wolf principal component analysis(PCA)

真菌polyporus sp. M05多糖诱导白血病细胞株HL-60凋亡作用机制的实验研究

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中华肿瘤防治杂志 2010年第6期17卷 431-433,445页

作者：张恒兰杨炜华温培娥任霞姜国胜孙晓柏苏恩裕姜国胜山东省医学科学院基础医学研究所山东省现代医用药物与技术重点实验室山东省医药卫生肿瘤免疫与中药免疫重点实验室济南250062

目的:研究真菌polyporus sp.M05多糖对白血病细胞株HL-60增殖抑制及诱导凋亡作用,并探讨其分子机制。方法:活细胞计数法检测多糖的增殖抑制作用;倒置显微镜下直接观察细胞形态;PI染色流式细胞术检测多糖诱导周期阻滞及凋亡作用;RT-PCR... 详细信息

目的:研究真菌polyporus sp.M05多糖对白血病细胞株HL-60增殖抑制及诱导凋亡作用,并探讨其分子机制。方法:活细胞计数法检测多糖的增殖抑制作用;倒置显微镜下直接观察细胞形态;PI染色流式细胞术检测多糖诱导周期阻滞及凋亡作用;RT-PCR检测凋亡相关基因。结果:多糖对HL-60细胞有增殖抑制作用,并随着浓度增大,作用增强;显微镜下观察细胞有明显的凋亡小体。流式细胞术检测细胞有明显G0/G1期阻滞及凋亡峰的出现,并随着时间越长和浓度的增大凋亡阳性率比例越高,与阴性对照相比差异有统计学意义(Ppolyporus sp.M05中提取的多糖对白血病细胞株HL-60有增殖抑制和诱导凋亡作用,分子机制与Bcl-2基因下调,Bax、Fas及Caspase-9基因上调有关,线粒体途径及死亡受体途径均参与了此凋亡过程。

关键词：真菌 polyporus 多糖 HL-60 凋亡

Inhibitive Effect of Umbellatus polyporus Polysaccharide on Cachexic Manifestation induced by toxohormone-L in Rats

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Chinese Journal of Integrative Medicine 1997年第3期3卷 173-173页

作者：吴耕书张荔彦奥田拓道 Shenyang Medical College Shenyang

Ｉｎｈｉｂｉｔｉｖｅ　Ｅｆｆｅｃｔ　ｏｆ　Ｕｍｂｅｌｌａｔｕｓ　Ｐｏｌｙｐｏｒｕｓ　Ｐｏｌｙｓａｃｃｈａｒｉｄｅ　ｏｎ　Ｃａｃｈｅｘｉｃ　Ｍａｎｉｆｅｓｔａｔｉｏｎ　ｉｎｄｕｃｅｄ　ｂｙ　ｔｏｘｏｈｏｒｍｏｎｅ－Ｌ　... 详细信息

Ｉｎｈｉｂｉｔｉｖｅ　Ｅｆｆｅｃｔ　ｏｆ　Ｕｍｂｅｌｌａｔｕｓ　Ｐｏｌｙｐｏｒｕｓ　Ｐｏｌｙｓａｃｃｈａｒｉｄｅ　ｏｎ　Ｃａｃｈｅｘｉｃ　Ｍａｎｉｆｅｓｔａｔｉｏｎ　ｉｎｄｕｃｅｄ　ｂｙ　ｔｏｘｏｈｏｒｍｏｎｅ－Ｌ　ｉｎ　ＲａｔｓＷＵＧｅｎｇ－ｓｈｕ...

关键词： polyporus Manifestation induced Cachexic Effect Polysaccharide Umbellatus by Inhibitive of

CURATIVE EFFECT OF ZHULING POLYSACCHARIDE INJECTION IN DEBILITATED CHILDREN WITH HYPOIMMUNITY

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Journal of Traditional Chinese Medicine 1991年第4期11卷 253-255页

作者：梅力张菊清于连娣王乃刚盛延秦严述常王少柏李业芳 Department of Pediatrics Beijing Hospital of Civil Aviation of China Institute of Chinese Materia Medica China Academy of Traditional Chinese Medicine

ZHULING POLYSACCHARIDE IN-JECTION(ZPI)is extracted from theChinese materia medica,polyporus *** application of this medicain the treatment of malignant tumors andhepatitis B was recently reported in the *** our hospit... 详细信息

关键词： latus polyporus materia ranged children IgG running globulin suffered immunity