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Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 660-665页

作者： Islamudin Ahmad Arry Yanuar Kamarza Mulia Abdul Mun'im Department of Pharmaceutical Sciences Faculty of Pharmacy Mulawarman University Samarinda East Kalimantan Indonesia Department of Pharmaceutical Sciences Faculty of Pharmacy Universitas Indonesia Depok 16424 West Java Indonesia Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 West Java Indonesia

Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.

关键词： Peperomia pellucida (L) Kunth 1-buthyl-3-methylimidazolium Tetrafluoroborate Response surface methodology Box–Behnken design polyphenolic content

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Bongardia chrysogonum(L.)Spach:a valuable source for nutraceutical and pharmacological industries

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Traditional Medicine Research 2022年第3期7卷 98-104页

作者： Sevgi Gezici Nazim Sekeroglu Department of Molecular Biology and Genetics Faculty of Science and LiteratureKilis 7 Aralik UniversityKilis 79000Turkey Advanced Technology Application and Research Center Kilis 7 Aralik UniversityKilis 79000Turkey Department of Horticulture Faculty of AgricultureKilis 7 Aralik UniversityKilis 79000Turkey

Background:Bongardia chrysogonum(L.)Spach,also known as“Uruf-el-Deek”,has been used by rural communities for the treatment of various diseases and disorders,owing to its wide array of secondary metabolite ***:The current study was conducted to determine the total polyphenolic content of Bongardia chrysogonum(L.)Spach as well as its potential free radical scavenging and metal-chelating antioxidant *** leaves,stems,and tubers were separately extracted using ethanol,water,and chloroform;their antioxidant activities were analyzed using in vitro models,including DPPH,ABTS,FRAP,and CUPRAC *** total polyphenolic content of the extracts was spectrophotometrically ***:The richest polyphenolic content of the extracts was yielded by tuber chloroform and leaf ethanol extraction(215.09±0.53 mg/g extract as gallic acid equivalent and 240.74±1.12 mg/g extract as quercetin equivalent,respectively).Almost all of the tested extracts exhibited remarkable antioxidant activities,with the highest antioxidant capacity observed in the leaf *** the other hand,the tuber ethanol extract demonstrated the weakest DPPH radical scavenging activity(%inhibition=49.75±0.38,Ppolyphenolic content and strong antioxidant ***,it could be a valuable source for the pharmaceutical industry in the prevention and treatment of diseases associated with oxidative stress.

关键词： Bongardia chrysogonum(L.)Spach polyphenolic content antioxidant medicinal plants

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