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Chinese Journal of Natural Medicines 2018年第5期16卷 375-400页

作者： HUANG Juan ZHANG Ju-Ping BAI Jun-Qi WEI Mei-Jin ZHANG Jing HUANG Zhi-Hai QU Guang-Hang XU Wen QIU Xiao-Hui The 2nd Clinical College of Guangzhou University of Chinese Medicine Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510006 China Shanghai Huayu Pharmaceutical Limited Company Shanghai 200000 China

The raw and processed roots of Plygonum multiflorum Thunb(PM) are used to treat different diseases in clinical practice. In order to clarify the influence of processing, a comparative study of chemical substance analysis was carried out. As the xenobiotics with a high enough exposure in target organs being considered as the potential effective or toxicity components, an in vivo study was also implemented to characterize the constitutes and metabolites, and meanwhile, the factor of compatibility with black bean were also considered. As a result, a total of 148 compounds were detected in PM extracts and more than 40 compounds were only detected in the processed products, which were probably new components produced during the steaming process. In in vivo study, 7 prototype components and 66 metabolites were detected or tentatively identified, 24 of which were reported for the first time. Our results indicated that processing greatly changed the chemical composition of PM and influenced the disposition of the compounds in vivo. To the best of our knowledge, this was the first global comparative study of raw and processed PM. These results expanded our knowledge about the influence of processing of PM and provided the essential data for further efficacy or toxicity studies.

关键词： polygoni Multiflori Radix Processing Chemical profiles In vivo metabolite study

Hematopoietic effect of small molecular fraction of polygoni multiflori Radix Praeparata in cyclophosphamide-induced anemia mice

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Chinese Journal of Natural Medicines 2019年第7期17卷 535-544页

作者： ZHU Li-Li FAN Luo-Di HU Ming-Hua MA Fang-Li QI Jin Jiangsu Key Laboratory of TCM Evaluation and Translational Research China Pharmaceutical University Nanjing 210009 China Infinitus Chinese Herbal Immunity Research Centre Infinitus (China) Company Limited. Guangzhou 510663 China

The aim of this study is to investigate the protective effects of a small molecular fraction(SMF) of polygoni multiflori Radix Praeparata(PMRP) in a cyclophosphamide(CTX) induced anemia mouse model. Small molecular fraction of PMRP was prepared and identified by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS). In pharmacology, we examined the peripheral hemogram and thymus and spleen index. The content of granulocyte-macrophage colony-stimulating factor(GM-CSF) in serum was mensurated by enzyme-linked immunosorbent assay(ELISA);The level of superoxide dismutase(SOD), catalase(CAT), total antioxidant capacity(T-AOC), and malondialdehyde(MDA) in serum and spleen tissue homogenate were detected, and glutathione peroxidase(GSH-PX) was assayed in spleen. The results show that SMF can significantly accelerate the recovery of peripheral hemogram, increase the activity of antioxidant enzymes and GM-CSF in serum and spleen. SMF also increases the number of spleen cells, improves bone marrow pathology. In conclusion, the SMF of PMRP promoted the recovery of hematopoietic function in a CTX-induced anemia mouse, which can support SMF to be used as an adjunct to chemotherapy to counteract its side effects.

关键词： polygoni multiflori Radix Praeparata Small molecular fraction Cyclophosphamide Hemopoiesis

Screening and Identifying Hepatotoxic Components in polygoni multiflori Radix and polygoni multiflori Radix Praeparata

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World Journal of Traditional Chinese Medicine 2019年第3期5卷 173-179页

作者： Guang-Ping Zhang Hai-Jing Zhang Teng-Fei Chen Hong-Ping Hou Ping Su Yun-Hang Gao Yi-Fei Yang Zu-Guang Ye Atomized Inhalation Preparation Research Center Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences

Objective: In this study, the hepatotoxic components of polygoni multiflori Radix and polygoni multiflori Radix Praeparata(known as Heshouwu [HSW] and Zhiheshouwu [ZHSW] in China, respectively) were screened, isolated, and identified. Materials and Methods: The ethanol extracts of HSW and ZHSW were separated into 80 fractions according to their polarity in the preparation liquid phase. Chang liver cell line was used to screen the toxic components of HSW and ZHSW in vitro. The obtained toxic mixture was further collected, isolated, and identified to confirm the hepatotoxic compounds of HSW and ZHSW. Results: The identifid hepatotoxic compounds include 2,3,5,4’-tetrah ydroxystilbene-2-O-β-D-glucoside, emodin, physcion-8-O-β-d-glucoside, physcion, and citreorosein, the fist two among them were the main components of HSW and ZHSW. After processing of HSW, the contents of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and emodin in ZHSW were signifiantly decreased. Conclusions: The traditional processing with herb has signifiant effects on the components, especially the toxic components, in the extract of HSW and is an effective method to reduce its toxicity.

关键词： 2,3,5,4’-tetra-hydroxystilbene-2-O-β-D-glucoside emodin hepatotoxicity polygoni multiflori Radix polygoni multiflori Radix Praeparata

Expression of a Bacterial Chitinase (ChiB) Gene Enhances Resistance against Erysiphae polygoniInduced Powdery Mildew Disease in the Transgenic Black Gram (Vigna mungoL.) (cv. T9)

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American Journal of Plant Sciences 2018年第8期9卷 1759-1770页

作者： D.K.Das Post Graduate Department of Biotechnology T.M.Bhagalpur UniversityBhagalpurIndia

To enhance the antifungal response of blackgram (Vigna mungo L.), transgenic plants were generated by transferring bacterial chitinase gene with a CaMV 35S promoter. The chopped multiple shoot cells developed on the cotyledonary node were transformed by Particle gun method. Thecalli were raised on the Murashige and Skoog (MS) modified media supplemented with 50 m·gl-1 kanamycin. The transformation efficiency was 13% approximately. The resultant shoot buds were selected and the antibiotic resistant transgenic plantlets were regenerated. The development of the transgenic plants from the shoot buds took about four to six months. The integration of the transgene was confirmed by PCR, RT-PCR, Southern and western blot analyses. The transgenic plants exhibited higher chitinase activity than the non-transformed ones. The chitinase activity was examined by native polyacrylamide in-gel assay. The transgenic plants showed fungal tolerance as evidenced by the delayed onset of the disease and smaller lesions following an in vitro inoculation of the powdery mildew pathogen (Erysiphae polygoni DC). The transgenic plants adapted well to the greenhouse and did not show any phenotypic alterations.

关键词： Particle Gun Bombardment Organogenesis Powdery Mildew Transformation Erysiphae polygoni DC

Radix polygoni Multiflori Praeparata and Dioscorea Bulbifera Rhizome Decoctions Display Combined Effects Detected by a Three-Probe Drug Cocktail with Substrates of Rat Hepatic Cytochrome P450 Enzymes

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Pharmacology & Pharmacy 2014年第7期5卷 736-746页

作者： Li Jiang Pingping Shan Hui Yu Jiayuan Tao Chunyan Gong Guoqing Shen Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing China

Objectives: Radix polygoni Multiflori Praeparata (RPMP) and Dioscorea Bulbifera Rhizomes (DBR) are used in Chinese herbal medicine and have been frequently reported for adverse reactions on liver. In this research, we aimed to evaluate in vivo effects of RPMP and DBR on rat cytochrome P450 enzymes (CYP1A2, CYP2E1 and CYP3A2) with their respective substrates as probes. Methods: Rats were orally administered RPMP, DBR and RPMP/DBR combination at 12, 10 and (12 + 10) g/kg, respectively, or saline as a control, once daily for 7 days. Thereafter, a cocktail containing 10 mg/kg caffeine, 20 mg/kg chlorzoxazone and 10 mg/kg dapsone was tail vein injected to rats. At defined time points, plasma drug concentrations were simultaneously evaluated by HPLC. Pharmacokinetic parameters simulated by DAS software were used to assess RPMP and/or DBR effects on cytochrome P450 enzymes activity. ANOVA and Dunnett’s test were used for data analysis. Results: Caffeine metabolism was enhanced in RPMP animals and reduced after pretreatment with DBR, but no effect was observed in RPMP/DBR combination group. Chlorzoxazone and dapsone metabolism was enhanced in both RPMP and DBR groups and consequently in combination group. The data suggested that RPMP independently induces rat CYP1A2, CYP2E1 and CYP3A2 activity, while DBR independently inhibits activity of rat CYP1A2 and induces that of CYP2E1 and CYP3A2. RPMP/DBR combination showed no significant benefit compared with the two drugs alone and even showed a neutralized effect in CYP1A2 activity. Conclusions: Caution is needed when RPMP and/or DBR are co-administered with drugs metabolized by human CYP1A2, CYP2E1 and CYP3A4.

关键词： Radix polygoni Multiflori Praeparata Dioscorea Bulbifera Rhizomes Cytochrome P450 Herb-Drug Interactions Three-Probe Drug Cocktail

Protective Effects and Action Mechanism of Total Flavonoids of polygoni PERFOLIATI HERBA on Rats with Nonalcoholic Steatohepatitis

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Medicinal Plant 2018年第5期9卷 66-70页

作者： Ya GAO Kefeng ZHANG Riming WEI Guilin Medical University

[Objectives]To study the protective effects and mechanism of polygoni PERFOLIATI HERBA on rats with nonalcoholic steatohepatitis( NASH). [Methods] Sixty rats were randomly divided into normal group,model group,metformin group( 0. 5 mg/kg),and high dose,medium dose,and low dose groups of total flavonoids of polygoni PERFOLIATI HERBA( 600,300,150 mg/kg). The standard feed was given to the normal group,and the model group and the total flavonoids groups were fed with high-fat diet for 13 weeks to establish the NASH rat model. At the 8 th week,the metformin group and the polygoni PERFOLIATI HERBA total flavonoids groups were given with the corresponding drug treatment for 6 weeks,blood was taken from the eyeball to collect liver tissue. Biochemical method was used to determine ALT,AST,TC,TG,HDL-c,LDL-c,SOD,MDA,GSH-Px activity or content in serum and HOMA-IR,and ELISA was used to measure the TNF-α,IL-1β and IL-6 content in liver tissue; Western blotting was used to detect the expression levels of AMPK,p-AMPK and ACC in liver tissue. [Results] Total flavonoids of polygoni PERFOLIATI HERBA could significantly decrease the activity or content of ALT,AST and MDA in serum of NASH rats( P polygoni PERFOLIATI HERBA have a good protective effect on NASH rats,and its mechanism may be related to the functions of regulating the lipid metabolism,alleviating insulin resistance,inhibiting oxidative stress,inhibiting inflammatory reaction and regulating AMPK and ACC protein expression.

关键词： Total flavonoids of polygoni PERFOLIATI HERBA Nonalcoholic steatohepatitis (NASH) Blood lipid Insulin resistance Oxidative stress Inflammatory factors

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超声波辅助提取虎杖中的白藜芦醇的工艺研究

安徽农业科学 2011年第22期39卷 13383-13384页

作者：张景亚李朋伟仝艳王宪龄河南中医学院药学院河南郑州450008

[目的]优选超声波辅助提取虎杖中白藜芦醇的最佳工艺。[方法]采用超声波提取,通过正交试验考察提取时间、提取温度、超声功率对虎杖中白藜芦醇提取率的影响。[结果]虎杖中提取白藜芦醇的最佳工艺为:以无水乙醇为提取剂,超声波的提取温度... 详细信息

[目的]优选超声波辅助提取虎杖中白藜芦醇的最佳工艺。[方法]采用超声波提取,通过正交试验考察提取时间、提取温度、超声功率对虎杖中白藜芦醇提取率的影响。[结果]虎杖中提取白藜芦醇的最佳工艺为:以无水乙醇为提取剂,超声波的提取温度为80℃,提取时间为45 min,提取功率为200 W。在此条件下,虎杖中白藜芦醇的提取率可以达到0.940%。[结论]用超声波辅助提取虎杖中的白藜芦醇,工艺稳定性好且简便易行,为有效提取白藜芦醇提供了一种安全可靠的方法。

关键词：虎杖(RHIZOMA ET RADIX polygoni CUSPIDATI) 白藜芦醇超声波正交试验

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何首乌叶愈伤组织的诱导和二苯乙烯苷含量的检测

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安徽农业科学 2011年第32期39卷 19759-19760,19792页

作者：范洪琼重庆三峡职业学院重庆万州404001

[目的]对何首乌叶进行愈伤组织诱导并检测叶愈伤组织中二苯乙烯苷的含量。[方法]在25℃暗箱培养条件下,将何首乌叶在含有浓度为1 mg/L 2,4-二氯苯氧乙酸(2,4-D)和浓度为0.4 mg/L萘乙酸(NAA)的MS诱导培养基中进行诱导,15 d后把诱导出的... 详细信息

[目的]对何首乌叶进行愈伤组织诱导并检测叶愈伤组织中二苯乙烯苷的含量。[方法]在25℃暗箱培养条件下,将何首乌叶在含有浓度为1 mg/L 2,4-二氯苯氧乙酸(2,4-D)和浓度为0.4 mg/L萘乙酸(NAA)的MS诱导培养基中进行诱导,15 d后把诱导出的愈伤组织转接到相同培养基中培养15 d,共转接3次,并通过HPLC法检测叶愈伤组织中二苯乙烯苷的含量。[结果]诱导出的愈伤组织生长状况良好,并且测得叶愈伤组织中二苯乙烯苷的含量为0.37 mg/g。[结论]由叶诱导出的愈伤组织中含有二苯乙烯苷,该愈伤组织可以用于进一步的生理生化研究。

关键词：何首乌(RADIX polygoni MULTIFLORI) 愈伤组织叶二苯乙烯苷

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高效液相色谱法测定虎杖中的白藜芦醇含量

安徽农业科学 2011年第32期39卷 19729-19730,19750页

作者：谢兵贵州民族学院化学与环境科学学院贵州贵阳550025

[目的]以乙醇为萃取剂,优化提取条件,建立高效液相色谱测定虎杖中的白藜芦醇的方法。[方法]色谱柱为phenomenex C18(250 mm×4.6 mm,5μm),流动相为无水甲醇-(浓度0.25%)乙酸(65∶35,V/V)等梯度洗脱,流速为0.8 ml/min,检测波长306 nm,... 详细信息

[目的]以乙醇为萃取剂,优化提取条件,建立高效液相色谱测定虎杖中的白藜芦醇的方法。[方法]色谱柱为phenomenex C18(250 mm×4.6 mm,5μm),流动相为无水甲醇-(浓度0.25%)乙酸(65∶35,V/V)等梯度洗脱,流速为0.8 ml/min,检测波长306 nm,以白藜芦醇的提取得率为评价指标,采用单因素试验和L25(54)正交试验法筛选最佳工艺条件。[结果]提取白藜芦醇的最佳工艺为:乙醇浓度为60%,料液比为1∶30(g/ml),萃取温度为55℃,萃取时间为15 min;在此条件下,提取的白藜芦醇的提取得率达到0.826μg/g。[结论]该方法简便、快捷、准确性高,适用于虎杖中的白藜芦醇的测定。

关键词：高效液相色谱虎杖(RHIZOMA ET RADIX polygoni CUSPIDATI) 白藜芦醇

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虎杖中白藜芦醇苷不同检测方法的比较

安徽农业科学 2012年第5期40卷 2659-2660,2663页

作者：陈爽周国海皮小武贺丽萍曹庸吉首大学林产化工工程湖南省重点实验室吉首大学张家界校区湖南张家界427000 泰州师范高等专科学校江苏泰州225300 华南农业大学食品学院广东广州510642

[目的]分析比较不同方法测定的人工栽培虎杖中白藜芦醇苷的含量差异。[方法]采用高效液相色谱法(HPLC)和毛细管电泳法(CE)对人工栽培虎杖中白藜芦醇苷进行测定,并对这2种方法的线性范围、定量重现性、最低检测限及分离分析时间进行比较... 详细信息

[目的]分析比较不同方法测定的人工栽培虎杖中白藜芦醇苷的含量差异。[方法]采用高效液相色谱法(HPLC)和毛细管电泳法(CE)对人工栽培虎杖中白藜芦醇苷进行测定,并对这2种方法的线性范围、定量重现性、最低检测限及分离分析时间进行比较研究。[结果]选定HPLC-FD为测虎杖中白藜芦醇苷的最佳检测方法。检测条件:色谱柱为Diamonsil-C18(250 mm×0.46 mm,5μm),激发波长为334 nm,发射波长为408 nm,流动相为浓度25%乙腈,流速为1.0 ml/min,柱温为25℃,进样量为10μl。[结论]高效液相色谱法是测定虎杖中白藜芦醇苷含量较理想的方法。

关键词：虎杖(RHIZOMA ET RADIX polygoni CUSPIDATI) 白藜芦醇苷高效液相色谱毛细管电泳