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Gene Expression of Ornithine Decarboxylase in Lung Cancers and Its Clinical Significance

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 639-645页

作者： Hui TIAN~1* Qing HUANG~1 Lm LI~1 Xian-Xi LIU~2 Yan ZHANG~2 1 Department of Thoracic Surgery,Shandong University Qi Lu Hospital,Jinan 250012,China 2 Department of Medicine,Medical Molecular Biology Experimental Center,Shandong University,Jinan 250012,China Department of Thoracic Surgery Shandong University Qi Lu HospitalJinan 250012 China Department of Medicine Medical Molecular Biology Experimental Center Shandong UniversityJinan 250012 China

Lung cancer is one of the most lethal cancers in China because of its high incidence and *** decarboxylase （ODC）,an important enzyme in polyamine biosynthesis,is increased incancer *** chemotherapeutic agents aimed at reducing ODC expression show inhibitory effects oncancer cell growth,so ODC can be useful in gene diagnosis and gene therapy of *** this study,weexamined the effect of antisense ODC on lung cancer cells.A-549 cells were infected with rAd-ODC/Ex3as,a recombinant adenovirus containing the cytomegalovirus promoter,green fluorescent protein gene and 120bp antisense *** cell cycle was evaluated by flow cytometry.A nude mouse xenograft model wasused in the tumorigenicity *** transcription-polymerase chain reaction,Western blot and immuno-histochernistry were used to study the expressions of ODC on lung *** was found that the growth ofcells infected with rAd-ODC/Ex3as was substantially inhibited and cells were arrested at *** with rAd-ODC/Ex3as can suppress tumor formation in a nude mouse xenograft *** of ODC mRNA and ODC protein levels in lung cancer tissues was significantly higher than thatin normal tissues （P<0.05）,which correlated significantly with the stage of lung cancer （P<0.05）.This studysuggested that rAd-ODC/Ex3as has antitumor activity in human lung cancer *** ODC gene might playan important role in lung cancer and the overexpression of ODC might be related to the occurrence anddevelopment of lung cancer.

关键词： polyamine biosynthesis lung neoplasm ornithine decarboxylase A-549 cell line

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Multi-omics analysis of attenuated variant reveals potential evaluation marker of host damaging for SARS-CoV-2 variants

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Science China(Life Sciences) 2024年第1期67卷 83-95页

作者： Guangshan Xie Lin Zhu Siwen Liu Cun Li Xin Diao Yanhao Zhang Xiuli Su Yuanyuan Song Guodong Cao Li Zhong Pui Wang Xiaojuan Liu Bobo Wing-Yee Mok Shusheng Zhang Dong-Yan Jin Jie Zhou Honglin Chen Zongwei Cai State Key Laboratory of Environmental and Biological Analysis Hong Kong Baptist UniversityHong Kong SARChina State Key Laboratory for Emerging Infectious Diseases and Department of MicrobiologyLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina College of Chemistry Zhengzhou UniversityZhengzhou 450001China School of Biomedical Sciences The University of Hong KongHong Kong SARChina HKBU Shenzhen Institute of Research and Continuing Education Shenzhen 518000China

SARS-CoV-2 continues to threaten human society by generating novel variants via mutation and *** high number of mutations that appeared in emerging variants not only enhanced their immune-escaping ability but also made it difficult to predict the pathogenicity and virulence based on viral nucleotide *** markers for evaluating the pathogenicity of new variants are therefore *** comparing host responses to wild-type and variants with attenuated pathogenicity at proteome and metabolome levels,six key molecules on the polyamine biosynthesis pathway including putrescine,SAM,dc-SAM,ODC1,SAMS,and SAMDC were found to be differentially upregulated and associated with pathogenicity of *** validate our discovery,human airway organoids were subsequently used which recapitulates SARS-CoV-2 replication in the airway epithelial cells of COVID-19 *** ODC1 as a proof-ofconcept,differential activation of polyamine biosynthesis was found to be modulated by the renin-angiotensin system(RAS)and positively associated with ACE2 *** experiments demonstrated that ODC1 expression could be differentially activated upon a panel of SARS-CoV-2 variants of concern(VOCs)and was found to be correlated with each VOCs’pathogenic ***,the presented study revealed the discriminative ability of key molecules on polyamine biosynthesis as a predictive marker for virulence evaluation and assessment of SARS-CoV-2 variants in cell or organoid *** work,therefore,presented a practical strategy that could be potentially applied as an evaluation tool for the pathogenicity of current and emerging SARS-CoV-2 variants.

关键词： SARS-CoV-2 multi-omics attenuated variant predictive pathogenic marker polyamine biosynthesis

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Exploring polyamines:Functions in embryo/fetal development

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Animal Nutrition 2017年第1期3卷 7-10页

作者： Tarique Hussain Bi'e Tan Wenkai Ren Najma Rahu Dildar Hussain Kalhoro Yulong Yin National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical AgricultureKey Laboratory of Agro-EcologyInstitute of Subtropical AgricultureThe Chinese Academy of Sciences University of Chinese Academy of Sciences Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients Hunan Collaborative Innovation Center of Animal Production Safety Department of Veterinary Microbiology Faculty of Animal Husbandry and Veterinary SciencesSindh Agriculture University

polyamines such as putrescine, spermidine, spermine and agmatine are aliphatic polycationic compounds present in all living cells, and are derived from amino acids, intestinal bacteria, exfoliated enterocytes and supported from diet. polyamines as the key compounds play essential role in cell proliferation, growth and differentiation. They also exert significant effects on embryonic development,implantation, embryonic diapause, placentation, angiogensis and fetal development. This review paper summarizes the functions of polyamines and embryo/fetus development and its regulatory mechanism which should help to provide some evidences for clinic.

关键词： polyamines polyamine biosynthesis Embryo/fetus development

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