检索结果-南通市图书馆

OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

Journal of Genetics and Genomics 2022年第5期49卷 481-491页

作者： Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Present address:Vector-borne Virus Research Center Fujian Province Key Laboratory of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China.

In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关键词： Rice pollen wall Lipid transfer protein Male sterility

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Less and shrunken pollen 1(LSP1) encodes a member of the ABC transporter family required for pollen wall development in rice(Oryza sativa L.)

引用

The Crop Journal 2020年第3期8卷 492-504页

作者： Tao Luo Ting Zou Guoqiang Yuan Zhiyuan He Wenjie Li Yang Tao Miaomiao Liu Dan Zhou Hongfeng Zhao Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Huainian Liu Lingxia Wang Ping Li Shuangcheng Li State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina State Key Laboratory of Hybrid Rice Rice Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina

pollen fertility is an agronomic trait that strongly influences rice yield. Recent studies have revealed that the development of the pollen wall is required for pollen fertility and is regulated by several genes. However, the mechanisms underlying pollen and pollen wall development in rice remain largely unknown. In the present study, a point mutation in a gene on chromosome 1 was identified that resulted in the production of less and shrunken pollen(LSP) and led to defects in pollen wall formation. This gene was named LSP1 and was found to encode a member of the adenosine triphosphate-binding cassette(ABC)transporter G subfamily, OsABCG3. Two other loss-of-function mutants of LSP1/OsABCG3,generated using CRISPR/Cas9 technology, showed the same male sterile phenotype. The LSP1/OsABCG3 gene showed a spatio-temporal expression pattern in the developing anthers, and is an ortholog of the Arabidopsis genes At ABCG1 and At ABCG16, which play an important role in pollen wall development. Mutation of LSP1/OsABCG3 affected the expression of several genes involved in pollen and pollen wall formation. These results suggest that LSP1/OsABCG3 is critical for normal pollen fertility and shed light on the molecular mechanisms underlying rice pollen wall development.

关键词： Rice pollen fertility pollen wall ABCG transporter OsABCG3

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Delayed callose degradation restores the fertility of multiple P/TGMS lines in Arabidopsis

引用

Journal of Integrative Plant Biology 2022年第3期64卷 717-730页

作者： Kai-Qi Wang Ya-Hui Yu Xin-Lei Jia Si-Da Zhou Fang Zhang Xin Zhao Ming-Yue Zhai Yi Gong Jie-Yang Lu Yuyi Guo Nai-Ying Yang Shui Wang Xiao-Feng Xu Zhong-Nan Yang College of Biological and Environmental Engineering Jingdezhen UniversityJiangxi 333000China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China

Photoperiod/temperature-sensitive genic male sterility(P/TGMS)is widely applied for improving crop production.Previous investigations using the reversible male sterile(rvms)mutant showed that slow development is a general mechanism for restoring fertility to P/TGMS lines in Arabidopsis.In this work,we isolated a restorer of rvms–2(res3),as the male sterility of rvms–2 was rescued by res3.Phenotype analysis and molecular cloning show that a point mutation in UPEX1 l in res3 leads to delayed secretion of callase A6 from the tapetum to the locule and tetrad callose wall degradation.Electrophoretic mobility shift assay and chromatin immunoprecipitation analysis demonstrated that the tapetal transcription factor ABORTED MICROSPORES directly regulates UPEX1 expression,revealing a pathway for tapetum secretory function.Early degradation of the callose wall in the transgenic line eliminated the fertility restoration effect of res3.The fertility of multiple known P/TGMS lines with pollen wall defects was also restored by res3.We propose that the remnant callose wall may broadly compensate for the pollen wall defects of P/TGMS lines by providing protection for pollen formation.A cellular mechanism is proposed to explain how slow development restores the fertility of P/TGMS lines in Arabidopsis.

关键词： callose degradation fertility restoration pollen wall pollen development P/TGMS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tapetum Degeneration Retardation is Critical for Aliphatic Metabolism and Gene Regulation during Rice pollen Development

引用

Molecular Plant 2008年第4期1卷 599-610页

作者： Da-Sheng Zhang Wan-Qi Liang Zheng Yuan Na Li Jing Shi Jue Wang Yu-Min Liu Wen-Juan Yu Da-Bing Zhang Shanghai Jiao Tong University-Shanghai Institutes for Biological Sciences-Pennsylvania State University Joint Center for Life Sciences School of Life Scienceand Biotechnology Key Laboratory of Microbial Metabolism Ministry of Education Shanghai Jiao Tong University Shanghai 200240 China Instrumental Analysis Center of Shanghai Jiao Tong University Shanghai 200240. China

As a complex wall system in flowering plants, the pollen outer wall mainly contains aliphatic sporopollenin; however, the mechanism for synthesizing these lipidic precursors during pollen development remains less well understood. Here, we report on the function of the rice tapetum-expressing TDR （Tapetum Degeneration Retardation） gene in aliphatic metabolism and its regulatory role during rice pollen development. The observations of transmission electron microscopy （TEM） and scanning electron microscopy （SEM） analyses suggested that pollen wall formation was significantly altered in the tdr mutant. The contents of aliphatic compositions of anther were greatly changed in the tdr mutant revealed by GC-MS （gas chromatography-mass spectrometry） testing, particularly less accumulated in fatty acids, primary alcohols, alkanes and alkenes, and an abnormal increase in secondary alcohols with carbon lengths from C29 to C3S in tdr. Microarray data revealed that a group of genes putatively involved in lipid transport and metabolism were significantly altered in the tdr mutant, indicating the critical role of TDR in the formation of the pollen wall. Also, a wide range of genes （236 in total--154 up-regulated and 82 down-regulated） exhibited statistically significant expressional differences between wild-type and tdr. In addition to its function in promoting tapetum PCD, TDR possibly plays crucial regulatory roles in several basic biological processes during rice pollen development.

关键词： tapetum pollen wall aliphatic metabolism microarray gene expression rice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lipid Metabolism: Critical Roles in Male Fertility and Other Aspects of Reproductive Development in Plants

引用

Molecular Plant 2020年第7期13卷 955-983页

作者： Xiangyuan Wan Suowei Wu Ziwen Li Xueli An Youhui Tian Zhongzhi International Institute of Agricultural Biosciences Biology and Agriculture Research CenterUniversity of Science and Technology BeijingBeijing 100024China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing Solidwill Sci-Tech Co.Ltd.Beijing 100192China

Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation.Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS).To date,many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle,pollen wall,and subcellular organelle membranes in anther wall layers have been identified and characterized.In this review,we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants.On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin,wax,sporopollenin,and tryphine\r\Arabidopsis,rice,and maize as well as other plant species,updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed.Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines(Oh43,W23,and B73),a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced.More,we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transcriptional levels.Finally,we highlight recent findings on lipid metabolism-related genes and their roles in other aspects of plant reproductive development.A comprehensive understanding of lipid metabolism,genes involved,and their roles in plant reproductive development will facilitate the application of lipid metabolism-related genes in gene editing,haploid and callus induction,molecular breeding and hybrid seed production in crops.

关键词： lipid metabolism genic male sterility(GMS) anther cuticle pollen wall plant reproductive development

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lipid metabolism: critical roles in male fertility and other aspects of reproductive development in plants

Lipid metabolism: critical roles in male fertility and other...

引用

第十九届中国作物学会学术年会

作者： Suowei Wu Ziwen Li Xueli An Youhui Tian Xiangyuan Wan Zhongzhi International Institute of agricultural Biosciences Biology and Agriculture Research Center University of Science and Technology Beijing

【Background】Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation. Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS). To date, many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle, pollen wall, and subcellular organelle membranes in anther wall layers have been identified and characterized. Here, we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants.【Method】Bioinformatics analysis of lipid metabolic genes was conducted using the available anther RNA-sequencing(RNA-seq) data from three maize inbred lines, Oh43, W23, and B73, which belong to the Lancaster, Minnesota 13, and Reid heterotic groups, respectively.【Results】On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin, wax, sporopollenin, and tryphine in Arabidopsis, rice, and maize as well as other plant species, updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed. We summarized lipid metabolism and related GMS genes that are critical for the development of the plant anther cuticle and pollen wall, as well as subcellular organelle membranes in anther wall layers. The biosynthesis of anther and pollen lipids mainly occurs in the tapetum, but increasing evidence suggests that it also takes place in the epidermis and endothecium of anthers;both the genetic control and self-assembly systems are presumed to function coordinately in anther cuticle and pollen exine formation. Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines(Oh43, W23, and B73), a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced. More, we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transc

关键词： lipid metabolism genic male sterility（GMS） anther cuticle pollen wall plant reproductive development

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：