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Different distributions of interstitial cells of Cajal and platelet-derived growth factor receptor-α positive cells in colonic smooth muscle cell/interstitial cell of Cajal/plateletderived growth factor receptor-α positive cell syncytium in mice

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World Journal of Gastroenterology 2018年第44期24卷 4989-5004页

作者： Chen Lu Xu Huang Hong-Li Lu Shao-Hua Liu Jing-Yu Zang Yu-Jia Li Jie Chen Wen-Xie Xu Department of Physiology Shanghai Jiao Tong University School of Medicine Department of Anesthesiology Ren Ji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Department of Pediatric Surgery Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

AIM To investigate the distribution and function of interstitialcells of Cajal(ICCs) and platelet-derived growth factor receptor-α positive(PDGFRα+) cells in the proximal and distal *** The comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes(CMMCs). The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread. Intracellular recordings were used to assess electrical field stimulation(EFS)-induced inhibitory junction potentials(IJP) on the colonic smooth muscle. Western blot analysis was used to examine the expression levels of ICCs and PDGFRα in the colonic smooth *** Treatment with NG-nitro-L-arginine methyl ester hydrochloride(L-NAME) significantly increased the CMMC frequency and spontaneous contractions, especially in the proximal colon, while treatment with MRS2500 increased only distal CMMC activity and smooth muscle contractions. Both CMMCs and spontaneous contractions were markedly inhibited by NPPB, especially in the proximal colon. Accordingly, CyPPA sharply inhibited the distal contraction of both CMMCs and spontaneous contractions. Additionally, the amplitude of stimulationinduced nitric oxide(NO)/ICC-dependent slow IJPs(sIJPs) by intracellular recordings from the smooth muscles in the proximal colon was larger than that in the distal colon, while the amplitude of electric field stimulationinduced purinergic/PDGFRα-dependent fast IJPs(fIJPs) in the distal colon was larger than that in the proximal colon. Consistently, protein expression levels of c-Kit and anoctamin-1(ANO1) in the proximal colon were much higher, while protein expression levels of PDGFRα and small conductance calcium-activated potassium channel 3(SK3) in the distal colon were much *** The ICCs are mainly distributed in the proximal colon and there are more PDGFRα+ cells are in the dis

关键词： Interstitial cells of Cajal platelet-derived growth factor receptor-α positive cells Smooth muscle cell/interstitial cell of Cajal/platelet-derived growth factor receptor-α positive cell syncytium Nitric oxide Purine

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