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Genome-wide Investigation of micro RNAs and Their Targets in Brassica rapa ssp. pekinensis Root with plasmodiophora brassicae Infection

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Horticultural Plant Journal 2016年第4期2卷 209-216页

作者： WEI Xiaochun XU Wen YUAN Yuxiang YAO Qiuju ZHAO Yanyan WANG Zhiyong JIANG Wusheng ZHANG Xiaowei Institute of Horticulture Henan Academy of Agricultural Sciences Crop Designing Center Henan Academy of Agricultural Sciences

Increasing evidence has revealed that micro RNAs play a pivotal role in the post transcriptional regulation of gene expression in response to pathogens in plants. However, there is little information available about the expression patterns of mi RNAs and their targets in Chinese cabbage(Brassica rapa ssp. pekinensis) under plasmodiophora brassicae stress. In the present study, using deep sequencing and degradome analysis, a genome-wide identification of mi RNAs and their targets during P. brassicae stress was performed. A total of 221 known and 93 potentially novel mi RNAs were successfully identified from two root libraries of one control(635-10CK) and P. brassicae-treated Chinese cabbage samples(635-10T). Of these, 14 known and 10 potentially novel mi RNAs were found to be differentially expressed after P. brassicae treatment. Degradome analysis revealed that the 223 target genes of the 75 mi RNAs could be potentially cleaved. KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway analysis suggested that the putative target genes of the mi RNAs were predominately involved in selenocompound metabolism and plant hormone signal transduction. Then the expression of 12 mi RNAs was validated by quantitative real-time PCR(q RT-PCR). These results provide insights into the mi RNA-mediated regulatory networks underlying the stress response to the plant pathogen P. brassicae.

关键词： micro RNAs Brassica rapa ssp pekinensis plasmodiophora brassicae deep sequencing

Understanding the resistance mechanism in Brassica napus to early infection by plasmodiophora brassicae

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Understanding the resistance mechanism in Brassica napus to ...

A qPCR system for plasmodiophora brassicae detection in multiple samples

中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： MEI Jia-qin GUO Zhen WANG Jin-hua FENG Yu-xia ZHANG Chun-yu SI Jun QIAN Wei CHEN Guo-kang College of Agronomy and Biotechnology Southwest University Academy of Agricultural Sciences Southwest University College of Plant Protection Southwest University College of Plant Science & Technology Huazhong Agricultural University College of Horticulture and Landscape Architecture Southwest University

Clubroot,caused by plasmodiophora brassicae,is one of the most important diseases of Brassicas *** the mechanism of plant resistance has become the basis for resistance varieties *** this study,transcriptome analysis was conducted in a clubroot resistant *** breeding line ’ ZHE-226’ and a susceptible rapeseed variety ’ Zhongshuang 11’ at 0,3,6,9 and 12 days after inoculation(dai) to study their different defense response induced by *** alteration was detected specifically in ZHE-226 as soon as the root hair infection happened,with significant promotion on many processes in particular cell division/cell cycle,DNA repair and synthesis,protein synthesis,signaling,antioxidation and secondary ***,biochemical and histochemical assays were carried out between two genotypes at each time point,implying an effective signaling in ZHE-226 in resistance to ***,*** fast initiation of receptor kinases by unknown effectors of *** may activate intercellular G proteins which may,together with the enhanced Ca signalling,promote ROS,PCD and HR,and affect hormone mediated signal ***,the resistant rapeseed possibly possesses high ability on maintaining homeostasis of auxin and cytokinin thus may repress the development of clubs and galls on root and contribute to maintain root *** study is of great significance in understanding resistant mechanism of *** against ***.

关键词： Brassica napus plasmodiophora brassicae resistance mechanism

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A qPCR system for Plasmodiophora brassicae detection in mult...

Study on Pathogenicity Difference of plasmodiophora brassicae Under Different Temperature and pH Conditions

中国植物病理学会2019年学术年会

作者：关格格邢曼竹 Piao Zhongyun 梁月 College of Plant Protection Shenyang Agricultural University College of Horticulture Shenyang Agricultural University

Clubroot is a soil-borne disease caused by plasmodiophora brassicae,which mainly attack the cruciferous plants and causes serious economic losses in the *** pathogen produces numerous resting spores that can survive and keep their pathogenicity in soil for seven and more *** survival characteristics of resting spores led to the difficult management of *** this study,a detection system of *** in multiple samples was established using the qPCR approach.A plasmid containing the qPCR target DNA sequence was constructed and used to generate a standard *** established qPCR system was used to measure the abundance of resting spores in samples containing resting spores,including the purified resting spores,soils,and roots and seeds from Brassica napus,*** and ***,*** sensitivity levels were analyzed with the baseline concentrations,including 1 000 copies/μL plasmid,10 resting spores and 1 000 spores/g soil,1 000 spores/g root and1 000 spores/g *** variation of the spore concentrations in the multiple samples was evaluated.A level of 1×10 spores showed the minimum variation with the positive correlation to a certain disease ***,a quantitative system of *** in the multiple contaminated samples was established,which will facilitate the accurate detection and timely monitoring of *** study also provides a technical approach and theoretical reference for the early forecast and epidemic in the sustainable management of clubroot.

关键词： Clubroot plasmodiophora brassicae quantitative detection Liner regression

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Agricultural Science & Technology 2015年第1期16卷 112-115页

作者：裴卫华李向东杨佩文曹继芬毕云青杨子林芮文马桂明林兴华周丽凤杨明英云南省农业科学院农业环境资源研究所云南昆明650205 临沧市植保植检站丽江市农产品质量安全检测中心云南丽江674100 丽江市植保植检站云南丽江674100

[Objective] This study was conducted to investigate the pathogenicity of plasmodiophora brassicae on cabbage grown under different temperature and soil pH conditions. [Method] The pathogenicity of P. brassicae were tested at seven different temperatures and at six different soil pH values with the resting spore concentration of lx108 （spores/g） in the soil. The plant survival rate and incidence rate of clubroot were investigated after 90 d. [Result] The incidence rate of clubroot on cabbage among the different temperature sets varied in a descending order as follows： 30 ℃〉25 ℃〉20 ℃〉35 ℃〉15 ℃〉10 ℃〉5 ℃ at soil pH value of 6, indicating that the pathogenicity of P. brassicae was weak at 5 and 10 ~（3. The incidence rate increased with soil temperature increasing from 15 to 30 ℃, but decreased at 35 ℃. The incidence rates of clubroot were 80.36%, 100%, 65%, 10.77%, 3.23% and 0% at soil pH 4, 5, 6, 7, 8 and 9 at 25 ℃, respectively. The growth of cabbage was inhibited and the survival rate was reduced at pH *** incidence rates of clubroot were low at pH value of 7 and 8, and was 0% at pH 9. The Chinese cabbage grew better at pH value of 5 and 6, but had high incidence rates of clubroot. [Conclusion] The results revealed that the incidence rate of clubroot on cabbage was closely related to the temperature and soil pH.

关键词： plasmodiophora brassicae Temperature pH value Pathogenicity

WeiTsing: a new face of Ca^(2+)-permeable channels in plant immunity

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Stress Biology 2023年第1期3卷 283-287页

作者： Feng-Zhu Wang Jian-Feng Li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China

Plants employ pattern-and effector-triggered immunity(PTI and ETI)to synergistically defend invading pathogens and insect *** PTI and ETI can induce cytosolic Ca^(2+)spikes,despite in different spatiotemporal patterns,to activate downstream Ca^(2+)-dependent immune signaling *** multiple families of Ca^(2+)-permeable channels at the plasma membrane have been uncovered,the counterparts responsible for Ca^(2+)release from intracellular stores remain poorly *** a groundbreaking paper published recently by Cell,the authors reported that WeiTsing,an Arabidopsis endoplasmic reticulum(ER)-resident protein that was specifically expressed in the pericycle upon plasmodiophora brassicae(Pb)infection,could form resistosome-like Ca^(2+)-conducting channel and protect the stele of Brassica crops from Pb *** the channel activity of WeiTsing was indispensable for its immune function,the findings highlight a previously underappreciated role of Ca^(2+)release from intracellular repertoire in promoting plant disease resistance.

关键词： Plant immunity Ca^(2+)-permeable channel Endoplasmic reticulum plasmodiophora brassicae Brassica crop

Distribution of Rapeseed Clubroot Disease in Hubei Province and Evaluation of Yield Loss

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Agricultural Science & Technology 2012年第4期13卷 775-777,F0003页

作者：任莉贾建光李铭刘凡程雨贵张明海陈坤荣方小平中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室湖北武汉430062 宜昌市农业科学研究院湖北宜昌443000

[Objective] The aim of this study was to reveal the distribution of rapeseed clubfoot disease in Hubei Province and to assess the yield loss caused by the pathogen, plasmodiophora brassicae. [Method] Field surveys were conducted in Shayang, Dangyang, Zhijiang, Yidu and Changyang of Hubei Province during 2009- 2011. Clubfoot disease of rapeseed samples from the surveyed locations were con- firmed by PCR detection and plants infection experiment. The factors of yield and plot yields influenced by clubroot disease were determined in plot experiment. [Re- salt] Rapeseed clubroots were confirmed in Zhijiang and Dangyang and not found in Shayang and Yidu. Clubroot was found in cruciferous vegetables but not in rapeseed d in Changyang. Infection of P. brassicae significantly decreased of the first effective branch numbers of rapeseed, effective pod number per plant, seed number per pod and 1 000-grain weight. Yield of infected rapeseed decreased by 56.4% over non-infected control. [Conclusion] Rapeseed clubroot disease mainly distributed in Zhijiang and Dangyang of Hubei Province. The disease had an economic impact on rapeseed production.

关键词： Brassica napus plasmodiophora brassicae Occurence Economic impact

Suppression of clubroot by Bacillus velezensis Pla6 via antibiosis and promoting growth of cabbages

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Suppression of clubroot by Bacillus velezensis Pla6 via anti...