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Study on Agrobacterium tumefaciens-mediated Transformation of Brassica campestris L. with Fusion Gene Ycoil-bFGF

Agricultural Science & Technology 2009年第4期10卷 31-36页

作者：徐岩肖艳双杜金霞汪洪郑伟李营庞实锋生物反应器与药物开发教育部工程研究中心吉林长春130118 吉林农业大学生命科学学院吉林长春130118 吉林农业大学中药材学院吉林长春130118 温州医学院药学院浙江温州325035

[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.

关键词： Basic fibroblast growth factor (bFGF) plant bioreactor Oil-body system Agrobacterium tumefaciens Brassica campestris L.

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Construction of Vectors to Express Foreign Protein within Potato Starch Grains

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Agricultural Science & Technology 2009年第4期10卷 72-74页

作者：李珺马力通姚新灵内蒙古科技大学内蒙古包头014010 宁夏大学生命科学学院宁夏银川750021

[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.

关键词： Foreign protein plant bioreactor GBSSI GC20 Starch grains

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