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In Vitro propagation and Artificial Seed Production of Fritillaria cirrhosa D. Don, an Endangered Medicinal plant

Phyton-International Journal of Experimental Botany 2024年第6期93卷 1297-1310页

作者： Qian Tao Guiqi Han Bujin Ma Hongmei Jia Can Zhao Wenshang Li Zhuyun Yan Chengdu University of Traditional Chinese Medicine State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest ChinaChengdu610000China

Fritillaria cirrhosa ***(Liliaceae)is an endangered perennial bulbous plant and its dry bulb is a valuable med-icinal material with antitussive and expectorant ***,lack of resources and expensive prices make it difﬁcult to meet clinical *** study presents a regeneration system aimed at overcoming the challenge of inadequate supply in ***,focusing on:(1)callus induction,(2)bulblets and adventitious bud induction,and(3)artiﬁcial seed *** development was achieved in 84.93%on Murashige and Skoog(MS)medium fortiﬁed with 1.0 mg·L^(-1) *** optimal medium for callus differentiation into regenerated bulb-lets was MS medium supplemented with 1.0 mg·L^(-1)6-benzyladenine(6-BA)and 0.2 mg·L^(-1)α-naphthaleneacetic acid(NAA).Subsequently,bulblets and adventitious buds were induced from regenerated bulblet sections cul-tured on MS medium fortiﬁed with 0.3 mg·L^(-1)6-BA+1.0 mg·L^(-1)2,4-dichlorophenoxyacetic acid(2,4-D),2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1),and the induction rates were 88.17%and 84.24%,*** regenerated bulblets were transplanted into a substrate of humus soil,river sand,and pearlite(1:1:1)after low-temperature *** germination rate was 42.80%after culture for 30 *** bulblets were used for encap-sulations in liquid MS medium containing 3%sucrose(w/v)+0.5 mg·L^(-1) NAA+2.0 mg·L^(-1)6-BA+3%sodium alginate(w/v)with a 10 min exposure to 2%CaCl_(2).Under non-aseptic conditions,the germination rate reached 81.67%,while the rooting rate was 20.56%after 45 *** capsule added 1.0 g·L^(-1) carbendazim and 1.0 g·L^(-1) activated carbon was the best component of artiﬁcial *** study successfully established an efﬁcient regen-eration system for the rapid propagation of ***,involving in vitro bulblet regeneration and artiﬁcial seed *** method introduces a novel approach for efﬁcient breeding and germplasm preservation,making it suitable for large-scale industrial resou

关键词： Artiﬁcial seed callus induction Fritillaria cirrhosa organogenesis plant propagation

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Effect of Eugenia winzerlingii Extracts on Bemisia tabaci and Evaluation of Its Nursery propagation

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 161-170页

作者： A.Cruz-Estrada E.Ruiz-Sánchez I.L.Medina Baizabal E.Balam-Uc M.Gamboa-Angulo Unidad de Biotecnología Centro de Investigación Científica de YucatánCalle 43 No.130Chuburna de HidalgoMérida CP 97205YucatánMéxico Tecnológico Nacional de México.Instituto Tecnológico de Conkal.Avenida Tecnológico s/n C.P.97345.ConkalYucatánMéxico

The development of plant-derived products to control Bemisia tabaci Genn.(Hemiptera:Aleyrodidae)is an urgent need for production of horticultural *** extracts and essential oils of several species of the genus Eugenia(Myrtaceae)have shown insecticidal *** southern Mexico,leaf extracts from Eugenia winzerlingii showed nematicidal effect but its insecticidal properties have not been ***,the objective of this study was to evaluate the insecticidal effect of aqueous and organic extracts from *** leaves on *** egg,nymph and adult stages,and else to explore its nursery ***,extracts of this species were obtained by maceration with different polarity *** were carried out on Capsicum chinense *** assays showed that aqueous and total crude ethanol(TCE)extracts necrosed the eggs(LC50=0.21%w/v and 4.68 mg/mL,respectively),whereas hexane,ethyl acetate(ETA),residual ethanol and TCE extracts affected the nymphs(LC50=0.25-4.85 mg/mL).In adults,oviposition inhibition by free choice assay indicated that TCE and ETA extracts had major activity(EC50=14.62 and 27.86μg/cm2,respectively).On other hand,the sexual and vegetative propagation of *** showed that this species can be easily cultivated by *** conclusion,extracts of *** leaves are highly effective in controlling *** extract,in particular,was toxic to three stages of *** plant could be a potential alternative to develop a novel botanical insecticide to manage this destructive pest.

关键词： Bemisia tabaci bioassays eugenia winzerlingii oviposition inhibition plant propagation

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Seed Germination Traits of Loquat (Eriobotrya japonica Lindl.) as Affected by Various Pre-Sowing Treatments (Cutting of Cotyledons, Removal of Perisperm, Moist Chilling and/or Exogenous Application of Gibberellin)

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 645-656页

作者： Thiresia-Teresa Tzatzani Evangelia Basdeki Evangelia-Vasiliki Ladikou Marios-Ioannis NSotiras Georgios Panagiotakis Sryridon Lionakis Ioannis E.Papadakis Laboratory of Subtropical plants and Tissue Culture Institute of Olive TreeSubtropical Plants and ViticultureHellenic Agricultural Organization-DEMETERChania73134Greece Laboratory of Genetics Department of BiotechnologyAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology Department of Crop ScienceAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology School of Agricultural SciencesHellenic Mediterranean UniversityHeraklion71410Greece

The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production in nurseries(applied research)as well as provide answers for important physiological issues related to loquat seeds and their seed coat(basic research).Three experiments were carried out with various pre-sowing *** treatments included full or partial removal of seed coat(perisperm),partial cutting of cotyledons as well as moist chilling at 5℃ for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid(GA_(3))solution for 24 *** to the results,cotyledons excision resulted in delayed germination,regardless of the presence or absence of the seed coat in comparison with the decoated seeds that demonstrated the highest germination rate amongst *** addition,even the partial excision of seed coats affected positively both the germinability and the germination rate,compared to the control-intact ***,control-intact seeds had a higher germination percentage when exposed to moist chilling independently of the application or not of gibberellin;while the combination of gibberellin application and moist chilling improved both the percentage and the rate of germination of decoated *** conclusion,the role of perisperm(seed coat)in the germination procedure of loquat seeds seems to be important,indicating the existence of seed coat-imposed dormancy on loquat ***,the existence of a mild endogenous embryo-dormancy on loquat is also discussed.

关键词： Cotyledon gibberellin seed coat seed testa Eriobotrya japonica plant propagation perisperm sexual propagation seedling dormancy

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Concentrations of Silver Nitrate in the In Vitro Development and Conservation of Passiflora gibertii ***

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American Journal of plant Sciences 2017年第12期8卷 2944-2955页

作者： Gláucia Amorim Faria Lucas Menezes Felizardo Antonio Flávio Arruda Ferreira Paula Suares Rocha Aline Namie Suzuki Antonio da Silva Souza Tatiana Góes Junghans Maria Angélica Pereira de Carvalho Costa Ana Patricia Bastos Peixoto Augusto Ramalho de Morais Beatriz Garcia Lopes Tiago Almeida de Oliveira Departmentof Mathematics School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Department of Civil Engineering School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Laboratory of Environmental Mutagenesis Institute of BiosciencesSao Paulo State University(Unesp)Rio ClaroBrazil Embrapa Cassava and Fruticulture Cruz das AlmasBrazil Center for Agrarian Biological and Environmental SciencesFederal University of the Recôncavo of Bahia(UFRB)Cruz das AlmasBrazil Department of Statistics State University of Paraíba(UEPB)Campina GrandeBrazil Department of Exact Sciences Federal University of Lavras(UFLA)LavrasBrazil

The aim of this study was to evaluate the effects of silver nitrate in the in vitro development and conservation of passion fruit plants. Experiments were carried out at the Laboratory of Culture and Tissues, in the National Cassava and Fruits Research Center (EMBRAPA), using microcuttings of Passiflora gibertii N. E. Br., previously cultivated in vitro. The microcuttings were placed in MS media supplemented with silver nitrate at concentrations of 0, 1, 2, 4 and 8 mg·L-1, and, during 30, 60 and 90 days, the following variables were identified: Shooting length (cm), number of roots, number and coloration of leaves. A completely randomized design with 30 replications in a split-plot scheme was employed. Polynomial regression equations were adjusted in analysis of variance to compare averages of treatments. The obtained results support the assumption of high sensitivity of passion fruit tissues to ethylene, which reflects in the lost of vigor and in the induction of culture senescence by its accumulation. Based on obtained results, and in order to mitigate this problem, it is possible to indicate an addition of 2 mg·L-1?of silver nitrate in culture media, for the micropropagation of passion fruit plant, allowing the controlling of the atmosphere inside the culture test tubes, during the establishment of explants and other steps of the process.

关键词： Passion Fruits Tropical Fruits plant propagation Reduced Growth Ethylene

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Synergic Effect of Mucuna pruriens ***(Fabaceae)and Pontoscolex corethrurus(Oligochaeta,Glossoscolecidae)on the Growth of Quercus insignis(Fagaceae)Seedlings,a Native Species of the Mexican Cloud Forest

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Open Journal of Forestry 2014年第1期4卷 1-7页

作者： Maria L.Avendano-Yánez Angel I.Ortiz-Ceballos Lázaro R.Sánchez-Velásquez María R.Pineda-López Jorge A.Meave Instituto de Biotecnología y Ecología Aplicada(INBIOTECA) Universidad VeracruzanaVeracruzMéxico Departamento de Ecología y Recursos Naturales Facultad de CienciasUniversidad Nacional Autonoma de MéxicoMexico D.F.México

propagation of native species in local nurseries is an important activity in reforestation and forest restoration programs. A requisite for successful plantation is that nursery produced plants are of a size and quality that allows optimal establishment under field conditions. Manipulation of edaphic processes through the combined use of the earthworm Pontoscolex corethrurus, Mucuna pruriens and inorganic fertilizers may promote faster biomass gain. This study assessed the activity of P. corethrurus, its association with M. pruriens (green manure) and inorganic fertilizers, on the growth of Quercus insignis seedlings under greenhouse conditions. Measured variables were basal diameter, height, biomass and foliar nitrogen content. Growth rates of basal diameter (F = 5.33;P F = 2.84;P P. corethrurus-M. pruriens-inorganic fertilizer, relative to the control. Also, leaf biomass and total biomass of the seedlings were greater in the treatment of P. corethrurus-fertilizer (F = 2.32;P F = 3.71;P Foliar nitrogen content

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