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Suspension Culture and Somatic Embryogenesis of Korean Pine

Phyton-International Journal of Experimental Botany 2022年第1期91卷 223-238页

作者： Chunxue Peng Fang Gao Hao Wang Iraida Nikolaevna Tretyakova Alexander Mikhaylovich Nosov Hailong Shen Ling Yang State Key Laboratory of Tree Genetics and Breeding School of ForestryNortheast Forestry UniversityHarbin150040China State Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine Harbin150040China Laboratory of Forest Genetics and Breeding Institution of the Russian Academy of SciencesV.N.Sukachev Institute of Forest Siberian Branch of RASKrasnoyarsk660036Russia Department of Cell Biology and Institute of Plant Physiology K.A.Timiryazev Russian Academy of SciencesMoscow127276Russia Department of Plant Physiology Biological FacultyLomonosov Moscow State UniversityMoscow119991Russia

Korean pine is an important afforestation tree species in Northeast China,which has a high ecological and economic *** regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful,it cannot be applied to automation and large-scale ***,we urgently need a method that can increase the output of somatic embryos(SEs)to meet the needs of large-scale *** used Korean pine 1-1 and 1-100 cell lines as research materials to evaluate the effects of inoculum-density,culture time,orbiting speed,vessel volume,plant growth regulator(PGR)concentration,and carbon source on the proliferation of embryogenic tissue(et).The somatic embryogenesis ability of et cultured in different liquid suspension media was also *** found that during liquid suspension culture of Korean pine et,the sedimented cell volume(SCV),fresh weight(FW)and dry weight(DW)were affected by inoculumdensity,culture time,orbiting speed,2,4-D concentration,6-BA concentration and carbon source *** mg⋅mL^(−1)et were transferred to a 200 mL Erlenmeyer flask containing 20 mL liquid medium,and cultured at 100 rpm/min for 14 days to obtain the maximum *** addition,we also found that SCV,FW and DW were higher when PGRs were reduced in the liquid suspension *** substitution of maltose for sucrose resulted in slow growth of cultures and limited SE yield(13 SEs g^(−1)FW).Although culture proliferation was high at 50 rpm,SE yield was inhibited by 48%compared with 100 rpm(50 rpm=33 SEs g^(−1)FW;100 rpm/min=70 SEs g^(−1)FW).Cultivation in low-concentration PGR(1.15μM⋅L^(−1)2,4-D,0.25μM⋅L^(−1)6-BA)and sucrose liquid medium at 100 rpm/min(80 SEs g^(−1)FW)could not only promote culture proliferation but also increase SE *** determination of the suspension culture scheme of Korean pine et provides a reference for further expansion to bioreactor culture in the future and lays a foundation for the automatio

关键词： pinus koraiensis sieb.et zucc embryogenic tissue liquid suspension medium cell proliferation

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