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Potential regulation of small RNAs on bacterial function activities in pig farm wastewater treatment plants

Journal of Environmental Sciences 2020年第5期32卷 292-300页

作者： Yiwen Yang Sicheng Xing Sumin Li Yajing Niu Cheng Li Tuoxin Huang Xindi Liao College of Animal Science South China Agricultural UniversityGuangzhou 510642China Key Laboratory of Tropical Agricultural Environment Ministry of AgricultureSouth China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding South China Agriculture UniversityGuangzhou 510642China

Small RNAs(sRNAs)are key players in the regulation of bacterial gene expression.However,the distribution and regulatory functions of sRNA in pig farm wastewater treatment plants(WWTPs)remains unknown.In this study,the wastewaters in anoxic and oxic tanks of the WWTPs were collected.The profiles of the community structure,mRNA expression,and sRNA expression of bacteria in pig farm wastewater were investigated using transcriptome sequencing and qPCR.This study demonstrated that there was a higher abundance of sRNA in the pig farm WWTPs and 52 sRNAs were detected.The sRNAs were mainly present in Proteobacteria and Firmicutes,including the potential human pathogenic bacteria(HPB)(Escherichia,Shigella,Bordetella and Morganella),crop pathogen(Pectobacterium)and denitrifying bacteria(Zobellella).And the sRNAs were involved in the bacterial functional activities such as translation,transcription,drug resistance,membrane transport and amino acid metabolism.In addition,most sRNAs had a higher abundance in anoxic tanks which contained a higher abundance of the genes associated with infectious diseases and drug resistance than that in oxic tanks.The results presented here show that in pig farm WWTPs,sRNA played an important role in bacterial function activities,especially the infectious diseases,drug resistance and denitrification,which can provide a new point of penetration for improving the pig farm WWTPs.

关键词： Small RNA Regulation Bacterial function pig farm Wastewater treatment plants

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Aerial pollutants on a pig farm in peri-urban Beijing,China

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International Journal of Agricultural and Biological Engineering 2013年第1期6卷 36-47页

作者： Roxana Mendoza Huaitalla Eva Gallmann Liu Xuejun Eberhard Hartung Institute of Agricultural Engineering University of HohenheimStuttgart70599Germany College of Agricultural Resources and Environmental Sciences China Agricultural UniversityBeijing 100094China Institute of Agricultural Engineering Christian-Albrechts-University KielKiel24098Germany

The study was carried out to evaluate the airborne gases and suspended particulate matter concentrations in the pig barns of a commercial pig farm situated in the peri-urban area of Beijing,China.The measurements followed the natural pig life-stages namely:gestation,farrowing,weaning,and fattening.In order to accomplish these objectives,three different measurement devices were employed:(i)color diffusion tubes,(ii)a portable gas measuring device,and(iii)a dust measuring instrument.Due to the rotation of the devices in the different sampling places,the measurements were performed at different time periods during the months of the summer and winter season in Beijing.The pig farm had a capacity of 15000 pigs per year and the manurial system was identified as“gan qing fen”or dry cleaning of the manure.The main by-products generated by the farm were irrigation water and small-scale biogas production.High dust concentrations were identified in the pig barns,especially during the feeding and manure cleaning events inside the farrowing and weaning barns with slatted floors.Inhalable dust ranged from 0 mg/m3 to 12.45 mg/m3,while the allowable dust ranged from 0 mg/m3 to 9.62 mg/m3.Ammonia concentration ranged from 0 ppm to 20 ppm(1 ppm=1 cm3/m3),and the carbon dioxide concentration ranged from 300 ppm to 8000 ppm.The highest ammonia concentration was recorded in the fattening barn during the summer season,while the highest carbon dioxide concentration was reported in the weaning barn during the summer season.The results of this study were similar to the results of studies performed on pig farms under natural and mechanical ventilation systems.On the other hand,the problems encountered during the gas measurements on the pig farm were directly related to the inadequate housing layout due to the obstruction of windows,fans and air channels for indoor ventilation,and thus the measurement devices faced harsh working conditions.Therefore,it is recommended that the housing system should be improved with repaired windows and curt

关键词： pig farm aerial pollutant barn China gases dust

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Assessment of immunization procedures for foot-and-mouth disease in large-scale pig farms in China based on actual data and dynamics

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Animal Diseases 2022年第1期2卷 45-60页

作者： Huarong Ren Zhen Jin Xin Pei Mingtao Li You ming Wang Juan Zhang Complex Systems Research Center Shanxi University030006 ShanxiChina Shanxi Key Laboratory of Mathematical Techniques and Big Data Analysis on Disease Control and Prevention 030006 ShanxiChina School of Mathematical Sciences Shanxi University030006 ShanxiChina College of Mathematics Taiyuan University ofTechnology030024 ShanxiChina The Laboratory of Animal Epidemiological Surveillance China Animal Health&Epidemiology Center266032 ShandongChina.

Foot-and-mouth disease(FMD)is an acute,highly infectious and pathogenic animal disease.In recent years,with the rapid development of the swine breeding industry in China,pig farms have shown a trend of larger-scale development.Large-scale pig farms employ standardized management,a high level of automation,and a strict_system.However,these farms have a large trading volume,and increased transmission intensity of FMD is noted inside the farm.At present,the main control measure against FMD is pig vaccination.However,a standard for immunization procedures is not available,and currently adopted immunization procedures have not been effectively and systematically evaluated.Taking a typical large-scale pig farm in China as the research subject and considering the breeding pattern,piggery structure,age structure and immunization procedures,an individual-based state probability model is established to evaluate the effectiveness of the immune procedure.Based on numerical simulation,it is concluded that the optimal immunization program involves primary immunization at 40 days of age and secondary immunization at 80 days of age for commercial pigs.Breeding boars and breeding sows are immunized 4 times a year,and reserve pigs are immunized at 169 and 259 days of age.According to the theoretical analysis,the average control reproduction number of individuals under the optimal immunization procedure in the farm is 0.4927.In the absence of immunization,the average is 1.7498,indicating that the epidemic cannot be controlled without immunization procedures.

关键词： pig farm Foot-and-mouth disease Individual-based state probability model Immunization procedure Infection probability Individual-based control reproduction number

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Study on Vetiver's Purification for Wastewater from pig farm

Study on Vetiver's Purification for Wastewater from Pig Farm

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作者： Xuhui Kong~2 Weiwen Lin~2 Biqing Wang~1 Fuhe Luo~2 (1.Floricultural Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China 2.Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

Nitrogen,phosphorus and heavy metals from pig farms are key sources of water pollution.Wastewater contains nitro-gen and phosphorus which are the most important plant nutrients,but is harmful when applied to agricultural land in excessamounts,thereby leading to pollution of ground water by nitrates,surface water by phosphorous(causing eutrophication)and soil byheavy metals such as copper which are used as growth promoters in the feed stuff.The main objective of this paper was to investi-gate the effects of vetiver(Vetiver zizanioides)in purifying wastewater from pig farms and design a purifying system for pig farmswith a Vetiver bamboo float.The results showed that Vetiver had a very high capacity to purify wastewater.It’s ratio of uptake andpurification to Cu and Zn>90%,to As and N>60%,to P was between 59～85%,to Pb was between 30～71%,and to Hg wasbetween 13～58%.The purifying effects of Vetiver to heavy metals,N,and P from pig farms were ranked as Zn>Cu>As>N>P>Pb>Hg.It is powerful to remove the elements of Cu,Zn,As and N for pig farms.

关键词： pig farm wastewater uptake purify Vetiveria zizanioides heavy metals

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Precision Livestock farming: Precision feeding technologies and sustainable livestock production

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International Journal of Agricultural and Biological Engineering 2012年第4期5卷 54-61页

作者： T M Banhazi L Babinszky V Halas M Tscharke National Centre for Engineering in Agriculture University of Southern QueenslandToowoombaQueensland4350 Australia Department of Feed and Food Biotechnology University of DebrecenH-4015 DebrecenHungary Department of Animal Nutrition Kaposvár UniversityH-7400 KaposvárHungary PLF Agritech Pty.Ltd.Queensland 4350 Australia

In order to be able to produce safe,uniform,cheap,environmentally-and welfare-friendly food products and market these products in an increasingly complex international agricultural market,livestock producers must have access to timely production related information.Especially the information related to feeding/nutritional issues is important,as feeding related costs are always significant part of variables costs for all types of livestock production.Therefore,automating the collection,analysis and use of production related information on livestock farms will be essential for improving livestock productivity in the future.Electronically-controlled livestock production systems with an information and communication technology(ICT)focus are required to ensure that information is collected in a cost effective and timely manner and readily acted upon on farms.New electronic and ICT related technologies introduced on farms as part of Precision Livestock farming(PLF)systems will facilitate livestock management methods that are more responsive to market signals.The PLF technologies encompass methods for electronically measuring the critical components of the production system that indicate the efficiency of resource use,interpreting the information captured and controlling processes to ensure optimum efficiency of both resource use and livestock productivity.These envisaged real-time monitoring and control systems could dramatically improve production efficiency of livestock enterprises.However,further research and development is required,as some of the components of PLF systems are in different stages of development.In addition,an overall strategy for the adoption and commercial exploitation of PLF systems needs to be developed in collaboration with private companies.This article outlines the potential role PLF can play in ensuring that the best possible management processes are implemented on farms to improve farm profitability,quality of products,welfare of livestock and sustainability of the farm environment,especiall

关键词： Precision Livestock farming(PLF) precision feeding control systems automation software-based technology sensors nutrition pig farm

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Disinfection Effects and Clinical Application of Sodium Dichloroisocyanurate Powder on Bacteria

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Agricultural Biotechnology 2022年第4期11卷 57-60页

作者： Shijin GUO Ling LI Qing LI Jingjing ZHOU Chengxin LYU Lanying GUO Zhiqiang SHEN Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Veterinary Drug Industry Binzhou 256600China Changyi Animal Husbandry Development Center Weifang 261300China Shandong Agriculture and Engineering University Jinan 250100China

With the continuous increase of large-scale pig farms,the disinfection of pig farm environment plays an extremely important part in the control and prevention of pig farm diseases in high-density breeding environment.As an important breeding place for pig farms,the farrowing house must be performed with scientific and standardized disinfection.In this paper,different disinfection modes were used to disinfect the empty farrowing houses of pig farms,and the total plate count,Escherichia coli,Staphylococcus aureus,and fungi were used as detection indicators to comprehensively evaluate the disinfection effect of the air microorganisms and the surface of the objects in the empty house after disinfection,with the aim to provide a basis for the establishment of a scientific pig farm environmental disinfection model.

关键词： Sodium dichloroisocyanurate powder Bacteria Disinfection effect pig farm Clinical application

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