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Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice

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Molecular Plant 2023年第11期16卷 1811-1831页

作者： Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang Liquan Kou Xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Life Sciences Shandong Agriculture UniversityTai’anShandong 271018China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China Yazhouwan National Laboratory SanyaHainan 572024China

Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(pi)***,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of pi and nitrogen remain largely ***,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental *** found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in ***,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under pi *** also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in ***,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus *** together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-pi environments.

关键词： rice pi deficiency strigolactone NSP1 and NSP2 CRL1 nitrogen and pi absorption

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Dissecting the function of ib-miR2111 and its targets in sweetpotato

Dissecting the function of ib-miR2111 and its targets in swe...

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2019年中国作物学会学术年会

作者：刘霞宇 Ruimin Tang Shifang Liu Jie Zhang Liheng He Runzhi Li Xiaoyun Jia College of Agriculture Shanxi Agriculture University College of Life Sciences Shanxi Agriculture University

【Introduction】Compared with white-fleshed sweetpotato(WFSP), purple-fleshed sweetpotato(PFSP) is a desirable resource for functional food development because of the abundant anthocyanin accumulation in its tuberous roots. Some studies have shown that the expression regulation mediated by miRNA plays an important role in anthocyanin biosynthesis in plants. However, few mi RNAs and their corresponding functions related to anthocyanin biosynthesis in tuberous roots of sweetpotato have been known. 【Materials and methods】By comprehensive analysis of transcriptome, small RNA(s RNA) and degradome data from the tuberous roots of WFSP(Xushu18) and PFSP(Xuzishu3), a set of sweetpotato miRNAs were highly associated with root-specific anthocyanin accumulation. In particular, there was a significant difference in the expression of ib-miR2111 and its target genes. Transgenic plants were generated for forward and reverse genetic investigation of ib-miR2111 and its corresponding targets, through over-expression and knockout by techniques such as STTM, RNAi, and CRISPR/Cas9. 【Results and analysis】 Two target genes of ib-miR2111, ibZFP and ibFbox were identified by degradome sequencing and RLM-5’RACE. The different expression and inverse correlation between the expression of ib-miR2111 and its targets in Xushu18 and Xuzishu3 were validated by qRT-PCR. The young seedlings of ib-miR2111 over-expressing Arabidopsis showed suppressed abundance of ibZFP and a purplish phenotype. under normal growth conditions. Transgenic sweetpotato seedlings overexpressing ib-miR2111 also appear purple. In addition, the transgenic Arabidopsis expressing p CBCDT1 T2-pHSE401-ib-miR2111 CRISPR/Cas9 vector showed decreased biomass. Overexpression and CRISPR/Cas9 vector of the two targets were constructed and transformed into sweetpotato callus cells. Interestingly, we found that ib-mi R2111 also responded to pi deficiency in sweetpotato.【Conclusion】Ib-mi R2111 was involved in the biosynthesis of anthocyanins

关键词： Sweetpotato Anthocyanin biosynthesis Ib-miR2111 pi deficiency

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