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Journal of Genetics and Genomics 2016年第4期43卷 199-206页

作者： Meng Li Zongxiang Tang Ling Qiu Yangyang Wang Shuyao Tang Shulan Fu Province Key Laboratory of Plant Breeding and Genetics Sichuan Agricultural University Agronomy College Sichuan Agricultural University

To effectively use elite genes on the long arm of rye chromosome 6（the 6RL arm） in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is ***-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat ***,only a limited number of 6RL-specific markers have been *** the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing（SLAF-seq） technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion ***,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was *** addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL *** markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding.

关键词： Wheat Rye 6RL-specific marker physical mapping SLAF-seq Powdery mildew

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RESTRICTION ENZYME ANALYSES AND physical mapping OF AN IBRV VARIANT DNA

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Progress in Natural Science:Materials International 1991年第4期 381-383页

作者：童光志 CAAS Harbin 150001 PRC The National Veterinary Biotechnological Laboratory Harbin Veterinary Research Institute

Infectious bovine rhinotracheitis(IBR)is an important disease of cattle and widely ex-ists in the whole *** investigations have indicated its epidemic in most partsof *** pathogen of the disease is an alpha herpesvirus,which is also called thebovine herpesvirus type 1(BHV-1).There are many isolates from different countries,the dif-

关键词： infectious bovine rhinotracheitis variant physical mapping

Fine mapping and physical mapping of a seed coat color gene in Chinese cabbage （Brassica rapa L.）

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Fine mapping and physical mapping of a seed coat color gene ...

Identification and physical mapping of QTLs associated with flowering time in Brassica napus L.

江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： Jiefu Zhang~(1,2),Genyi Li~2 1 Institute of Industry Crops,Jiangsu Academy of Agricultural Science,Nanjing 210014,China 2 Department of Plant Science,University of Manitoba,Winnipeg,R3T2N2,Canada

＜正＞Two DH lines in Chinese cabbage（***） were used as parents to develop a double haploid （DH） population that segregated for seed coat color *** segregation data suggested that one Mendelian locus controled the seed coat color trait in this *** testing 1100 primer combinations,14 SRAP markers were found to be linked to the seed coat color *** sequences of two closely linked SRAP molecular markers matched with annotated genes in *** clones anchoring these two markers were selected to confirm the gene order and colinearity between *** and *** Arabidopsis genome sequence as a reference,chromosome walking to the yellow-seeded gene was *** and SCAR molecular markers were developed and,together with the original SRAP molecular markers,a fine genetic map was *** map was aligned with its corresponding Arabidopsis physical map,showing that the gene order in this syntenic region containing the seed coat color gene in *** is the same as that in Arabidopsis. The closest molecular marker,SNP24650,was 0.2 cM away from the seed coat color *** next step is to find the candidate gene and to confirm its function through complementary transformation.

关键词： Fine mapping physical mapping yellow-seeded gene Chinese cabbage

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Identification and physical mapping of QTLs associated with ...

From free chromatin analysis to high resolution fiberFISH

江苏省遗传学会2019年学术研讨会

作者： Kunjiang Yu Xiaodong Wang Feng Chen Wei Zhang Jiefu Zhang Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences

Flowering time regulation is crucial to yield of rapeseed（Brassica napus L.）. However, insight into the loci controlling flowering time variation in rapeseed is not clear enough. In this study, a recombinant inbred line population was used for quantitative trait locus（QTL） analysis associated with flowering time in five different environments. A total of 36 identified QTLs were obtained, and each explained 3.96–24.41% of the phenotypic variation（PV）. The QTL meta-analysis showed that 36 identified QTLs were integrated into 24 consensus QTLs. Of these, eight consensus QTLs were stably expressed in at least two environments, and four with PV ≥ 10% were specifically expressed in a single environment. These 12 consensus QTLs were considered the key QTLs controlling flowering time in the AH population. physical mapping indicated that the 12 key QTLs were mapped into 12 single genomic fragments in B. rapa and B. oleracea, and physical positions of these QTLs in the B. napus genome were further determined by referring to the B. rapa and B. oleracea genomes. Ultimately, seven candidate genes underlying the confidence intervals of five key QTLs were identified. These findings will contribute to further insight into the genetic architecture of flowering time in rapeseed.

关键词： Brassica napus L Flowering time QTL physical mapping

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Cell Research 1997年第1期7卷 119-124页

作者： HENG HENRY HQ XIAO MEI SHI(Department of Biology, York University, Downsview,ON, Canada)(SeeDNA Biotech inc., Downsview, ON, Canada)(Department of Genetics, The Hospital of Sick Children, Toronto ON, Canada) Department of Biology York University Downsview Canada SeeDNA Biotech Inc. Downsview Canada Department of Genetics The Hospital of Sick Children Toronto Canada

Fluorescent in situ hybridization (FISH) methodology is one of the most powerful and versatile experimental systems for current biology. It bridges the molecular biology and cytology by providing direct and precise research tools for gene mapping, chromosomal structure study and genome analysis[1]. One major breakthrough for FISH analysis, is the introduction of the concept and methodologies of high resolution fiber FISH[2, 3]. By FISH detecting on released, less condensed, and linearized chromatin or DNA fibers, the ultra high resolution mapping data can be generated over the conventional chrqmosome-based FISH or interphase FISH [4], making a significant contribution for current genome project. This novel approach also provides an ideal tool for chromosome and genome structure and function studies [5-8].

关键词： Free chromatin fiber-FISH physical mapping chromosome and genome

mapping of Microsatellite SW943 to Porcine Chromosome 12p11-(2/3p13) Using Primed in situ Synthesis and Somatic Cell Hybrid Panel

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Agricultural Sciences in China 2002年第5期1卷 589-593页

作者： LIU Bang, WANG Yong-qiang, ZHANG Qing-de, YU Mei, ZHAO Shu-hong, XIONG Tong-an and LI Kui(Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070 ,P. R . China) Laboratory of Molecular Biology and Animal Breeding School of Animal Science and Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China

The porcine microsatellite SW943 was regionally localized on 12p11-(2/3p13) by the two methods: the Primed in situ (PRINS) labelling on the pachytene bivalents of pigs using the Dig-11-dUTP as the report molecule and pig X rodent Somatic Cell Hybrid Panel (SCHP) which contains 27 cell lines through PCR amplification. Advantages and disadvantages of the two methods for physical mapping of microsatellites were also discussed.

关键词： Primer in situ synthesis Somatic cell hybrid panel Microsatellites physical mapping Pigs

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大白菜种皮颜色基因的精细定位和物理图谱(英文)

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大白菜种皮颜色基因的精细定位和物理图谱(英文)