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Optimizing hairy root production from explants of phyllanthus hainanensis,a shrub used for traditional herbal medicine

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Frontiers of Agricultural Science and Engineering 2020年第4期7卷 513-522页

作者： Zhaogui YAN Shengyu LIU Junlian ZHANG Guan HUANG Lijun DUAN Yaomei YE College of Horticulture and Forestry Sciences/Hubei Engineering Technology Research Center for Forestry Information Huazhong Agricultural UniversityWuhan 430070China School of Environmental&Rural Science The University of New EnglandArmidaleNSW 2351Australia Wuhan Garden Constructional Engineering Company Wuhan 430050China Hubei Wildlife and Plant Conservation Agency Wuhan 430070China

phyllanthus hainanensis is a shrub that has been used in traditional herbal *** has great pharmaceutical potential for treating diseases such as cancer and *** a prerequisite for propagation of this species on a large scale,hairy roots in *** were induced using Rhizobium rhizogenes and various factors affecting hairy root induction and growth *** factors were tested:(1)type of explant,(2)type of culture medium,(3)duration of pre-culture,(4)*** inoculum cell density,(5)duration of infection,(6)acetosyringone concentration in the culture medium,and(7)duration of *** optimal protocol for hairy root induction and growth was:young shoots,pre-cultured in Y1 for 2 d,inoculated with *** broth with an OD600 of 0.6 for 20 min,and incubated for 3 *** transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain *** and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of *** able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.

关键词： hairy root induction medicinal plant MS growth medium phyllanthus hainanensis Rhizobium rhizogenes

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