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A unique photosystem i reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina

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Journal of integrative Plant Biology 2021年第10期63卷 1740-1752页

作者： Caihuang Xu Qingjun Zhu Jing-Hua Chen Liangliang Shen Xiaohan Yi Zihui Huang Wenda Wang Min Chen Tingyun Kuang Jian-Ren Shen Xing Zhang Guangye Han Department of Biophysics and Department of Pathology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310058China Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyThe Chinese Academy of SciencesBeijing 100093China College of Life Sciences University of Chinese Academy of ScienceBeijing 100049China Center of Cryo-Electron Microscopy Zhejiang University School of MedicineHangzhou 310058China School of Life and Environmental Science Faculty of ScienceUniversity of SydneySydney NSW2006Australia Division of Photosynthesis and Structural Biology Institute for Interdisciplinary Scienceand Graduate School of Natural Science and TechnologyOkayama UniversityOkayama 700-8530Japan

photosystem i(PSi)is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin(or cytochrome c6)and the reduction of ferredoxin.This catalytic reaction is realized by a transmembrane electron transfer chain consisting of primary electron donor(a special chlorophyll(Chl)pair)and electron acceptors A_(0),A_(1),and three Fe_(4)S_(4) clusters,F_(X),F_(A),and F_(B).Here we report the PSi structure from a Chl d-dominated cyanobacterium Acaryochloris marina at 3.3Åresolution obtained by single-particle cryo-electron microscopy.The A.marina PSi exists as a trimer with three identical monomers.Surprisingly,the structure reveals a unique composition of electron transfer chain in which the primary electron acceptor A_(0) is composed of two pheophytin a rather than Chl a found in any other well-known PSi structures.A novel subunit Psa27 is observed in the A.marina PSi structure.in addition,77 Chls,13α-carotenes,two phylloquinones,three Fe-S clusters,two phosphatidyl glycerols,and one monogalactosyl-diglyceride were identified in each PSi monomer.Our results provide a structural basis for deciphering the mechanism of photosynthesis in a PSi complex with Chl d as the dominating pigments and absorbing far-red light.

关键词： A.marina chlorophyll d cryoelectron microscopy pheophytin a photosynthesis photosystem i reaction center

Cryo-EM structure of a tetrameric photosystem i from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium

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Plant Communications 2022年第1期3卷 53-70页

作者： Dmitry A.Semchonok Jyotirmoy Mondal Connor J.Cooper Katrina Schlum Meng Li Muhamed Amin Carlos O.S.Sorzano Erney Ramirez-Aportela Panagiotis L.Kastritis Egbert J.Boekema Albert Guskov Barry D.Bruce Groningen Biomolecular Sciences&Biotechnology institute University of GroningenGroningenthe Netherlands Biochemistry&Cellular and Molecular Biology Department University of TennesseeKnoxvilleTNUSA Program in Genome Science and Technology University of TennesseeKnoxvilleTNUSA Bredesen Center for interdisciplinary Research&Education University of TennesseeKnoxvilleTNUSA Department of Sciences University College GroningenGroningenthe Netherlands Biocomputing Unit National Center for Biotechnology(CSIC)Darwin 3Campus Universidad Auto´noma de MadridCantoblanco28049 MadridSpain Universidad CEU San Pablo Campus Urb.Monteprı´ncipeBoadilla del Monte28668 MadridSpain institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany Microbiology Department University of TennesseeKnoxvilleTNUSA Present address:institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany School of Oceanography University of WashingtonSeattleWAUSA

photosystem i(PSi)is one of two photosystems involved in oxygenic photosynthesis.PSi of cyanobacteria exists in monomeric,trimeric,and tetrameric forms,in contrast to the strictly monomeric form of PSi in plants and algae.The tetrameric organization raises questions about its structural,physiological,and evolutionary significance.Here we report the3.72 A˚resolution cryo-electron microscopy structure of tetrameric PSi from the thermophilic,unicellular cyanobacterium Chroococcidiopsis sp.TS-821.The structure resolves 44 subunits and 448 cofactor molecules.We conclude that the tetramer is arranged via two different interfaces resulting from a dimer-of-dimers organization.The localization of chlorophyll molecules permits an excitation energy pathway within and between adjacent monomers.Bioinformatics analysis reveals conserved regions in the PsaL subunit that correlate with the oligomeric state.Tetrameric PSi may function as a key evolutionary step between the trimeric and monomeric forms of PSi organization in photosynthetic organisms.

关键词： cryo-EM photosystem i non-heterocyst-forming cyanobacteria Chroococcidiopsis evolution of chloroplast high light adaptation

Photoinduced hydrogen evolution in an artificial system containing photosystem i, hydrogenase, methy1 viologen and mercaptoacetic acid

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Chinese Chemical Letters 2008年第5期19卷 607-610页

作者： Dong Jin Qian mi Rong Liu Chikashi Nakamura Stephan Olav Wenk Jun Miyake Department of Chemistry Fudan University 220 Handan Road Shanghai 200433 China Tissue Engineering Research Center National Institute of Advanced Industrial Science and Technology 3-11-46 Nakouji Amagasaki Hyogo 661-0974 Japan

Hydrogen evolution was detected in an artificial system composed of light-harvesting unit of purified photosystem i, catalyst of hydrogenase, methyl viologen and electron donor under radiation. Absorption spectral features confirmed that electron transfer from electron donors to proton was via a photoinduced reductive process of methyl viologen.

关键词： Hydrogen evolution Hydrogenase photosystem i Artificial system

Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem Ⅰfrom Arabidopsis thaliana

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Molecular Plant 2022年第3期15卷 454-467页

作者： Xiaodong Su Duanfang Cao Xiaowei Pan Lifang Shi Zhenfeng Liu Luca Dall’Osto Roberto Bassi Xinzheng Zhang Mei Li National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China College of Life Science Capital Normal UniversityBeijing 100101P.R.China Dipartimento di Biotecnologie Universitàdi Verona37134 VeronaItaly Center for Biological imaging CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101P.R.China

Cyclic electron transport/flow(CET/CEF)in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency.A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like(NDH)complex that contains at least 29 protein subunits and associates with photosystem i(PSi)to form the NDH-PSi supercomplex.Here,we report the 3.9Åresolution structure of the Arabidopsis thaliana NDH-PSi(AtNDH-PSi)supercomplex.We constructed structural models for 26 AtNDH subunits,among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex.in the supercomplex,one NDH can bind up to two PSi-light-harvesting complex i(PSi-LHCi)complexes at both sides of its membrane arm.Two minor LHCis,Lhca5 and Lhca6,each present in one PSi-LHCi,interact with NDH and contribute to supercomplex formation and stabilization.Collectively,our study reveals the structural details of the AtNDH-PSi supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.

关键词： cyclic electron transport/flow NADH dehydrogenase-like(NDH)complex photosystem i structure Arabidopsis thaliana

Quantitative proteomics reveals redox-based functional regulation of photosynthesis under fluctuating light in plants

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Journal of integrative Plant Biology 2022年第11期64卷 2168-2186页

作者： Qi Chen Yixian Xiao Yu Ming Rong Peng Jiliang Hu Hong-Bin Wang Hong-Lei Jin institute of Medical Plant Physiology and Ecology School of Pharmaceutical SciencesGuangzhou University of Chinese MedicineGuangzhou 510006China

Photosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant cells.Fluctuating light(FL) levels,which occur commonly in natural environments,affect photosynthesis;however,little is known about the specific effects of FL on the redox regulation of photosynthesis.Here,we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL conditions.We identified8857 redox-switched thiols in 4350 proteins,and1501 proteins that are differentially modified depending on light conditions.Notably,proteins related to photosynthesis,especially photosystem i(PSi),are operational thiol-switching hotspots.Exposure of wild-type A.thaliana to FL resulted in decreased PSi abundance,stability,and activity.interestingly,in response to PSi photodamage,more of the PSi assembly factor PSA3 dynamically switches to the reduced state.Furthermore,the Cys199 and Cys200 sites in PSA3 are necessary for its full function.Moreover,thioredoxin m(Trx m) proteins play roles in redox switching of PSA3,and are required for PSi activity and photosynthesis.This study thus reveals a mechanism for redox-based regulation of PSi under FL,and provides insight into the dynamic acclimation of photosynthesis in a changing environment.

关键词： fluctuating light photosystem i photosynthesis redox regulation thioredoxin

Nitric Oxide Alleviates Photochemical Damage induced by Cadmium Stress in Pea Seedlings

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Phyton-international Journal of Experimental Botany 2022年第5期91卷 959-973页

作者： Ekhlaque A.Khan Hamdino M.i.Ahmed Meena Misra Pallavi Sharma Amarendra N.Misra Department of Life Sciences Central University of JharkhandRanchi835205India Department of Biotechnology Ch.Bansilal UniversityBhiwaniHaryana127021India Horticulture Research institute(HRi) Agricultural Research Center(ARC)Giza12619Egypt School of Environment and Sustainable Development Central University of GujaratGandhinagar382030India

Cadmium(Cd),a life threatening hazardous heavy metal is abundant in nature.Cd amounts are greater in leaves than other plant parts,and it shows considerable effects on photosynthesis.Nitric oxide(NO),a free radical present in living organisms,is now known as an important signaling molecule playing various physiological processes in plants.in this study,the possible ameliorative effect of NO on photosynthesis was examined on pea seedlings grown under Cd stress.Results showed that chlorophyll,net photosynthetic rate,transpiration rate,stomatal conductance,photochemical efficiency of photosystem ii and photosystem i decreased,and Fo and nonphotochemical parameters for PSii and PSi significantly increased due to Cd stress.This suggests that Cd affects the photochemistry efficiency at both the PSii and PSi levels.Nitric oxide supplementation through SNP ameliorated Cd stress by enhancing all the above mentioned parameters but causing a reduction in the Fo,and non-photochemical parameters of PSii and PSi in pea plants.These data indicate that the exogenous application of NO was useful in mitigating Cd-induced damage to photosynthesis in pea seedling.

关键词： Chlorophyll fluorescence oxidative stress photosystem i photosystem ii

Yang cycle enzyme DEP1: its moonlighting functions in PSi and ROS production during leaf senescence

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Molecular Horticulture 2022年第1期2卷 133-148页

作者： Chu-Kun Wang Xiu-Ming Li Fang Dong Cui-Hui Sun Wen-Li Lu Da-Gang Hu National Key Laboratory of Crop Biology MOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’an 271018ShandongChina Shandong institute of Pomology Key Laboratory for Fruit Biotechnology Breeding of ShandongTai’an 271000ShandongChina

Ethylene-mediated leaf senescence and the compromise of photosynthesis are closely associated but the underlying molecular mechanism is a mystery.Here we reported that apple DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1(MdDEP1),initially characterized to its enzymatic function in the recycling of the ethylene precursor SAM,plays a role in the regulation of photosystem i(PSi)activity,activating reactive oxygen species(ROS)homeostasis,and negatively regulating the leaf senescence.A series of Y2H,Pull-down,CO-iP and Cell-free degradation biochemical assays showed that MdDEP1 directly interacts with and dephosphorylates the nucleus-encoded thylakoid protein MdY3iP1,leading to the destabilization of MdY3iP1,reduction of the PSi activity,and the overproduction of ROS in plant cells.These findings elucidate a novel mechanism that the two pathways intersect at MdDEP1 due to its moonlighting role in destabilizing MdY3iP1,and synchronize ethylene-mediated leaf senescence and the compromise of photosynthesis.

关键词： DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1 Yang cycle photosystem i ROS Leaf senescence Photosynthesis

Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves

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Journal of integrative Agriculture 2016年第1期15卷 87-100页

作者： MiAO Yan-xiu W ANG Xiao-zhuo G AO Li-hong C HEN Qing-yun QU Mei Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops College of Agriculture and Biotechnology China Agricultural UniversityBeijing 100193P.R.China

Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the regulation mechanism of related photosynthesis characteristics remains largely unclear. Here, four light qualities treatments （300 μmol m-2 s-1） including white fluorescent light （FL）, blue monochromatic light （B, 440 nm）, red monochromatic light （R, 660 nm）, and a combination of red and blue light （RB, R：B=8：1） were carried out to investigate their effects on the activity of photosystem ii （PSii） and photosystem i （PSi）, and photosynthetic electron transport capacity in the leaves of cucumber （Cucumis sativus L.） seedlings. The results showed that compared to the FL treatment, the R treatment significantly limited electron transport rate in PSii （ETR11） and in PSi （ETR1） by 79.4 and 66.3%, respectively, increased non-light induced non-photochemical quenching in PSii （q^No） and limitation of donor side in PSi （φND） and reduced most JiP-test parameters, suggesting that the R treatment induced suboptimal activity of photosystems and inhibited electron transport from PSii donor side up to PSi. However, these suppressions were effectively alleviated by blue light addition （RB）. Compared with the R treatment, the RB treatment significantly increased ETR, and ETR1 by 176.9 and 127.0%, respectively, promoted photosystems activity and enhanced linear electron transport by elevating electron transport from QA to PSi. The B treatment plants exhibited normal photosystems activity and photosynthetic electron transport capacity similar to that of the FL treatment. it was concluded that blue light is more essential than red light for normal photosynthesis by mediating photosystems activity and photosynthetic electron transport capacity.

关键词： cucumber photosystem ii photosystem i electron transport