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Engineering photocleavable protein-decorated Hydrogels to Regulate Cell Adhesion and Migration

Chemical Research in Chinese Universities 2022年第6期38卷 1512-1517页

作者： LI Tianyi HUANG Yaying LU Chenjing GU Liwen CAO Yi YIN Sheng Collaborative Innovation Center of Advanced Microstructures National Laboratory of Solid State MicrostructureDepartment of PhysicsNanjing UniversityNanjing210093P.R.China Jurong Country Garden School Zhenjiang

Cell adhesion and migration play essential roles in tissue development and maintenance, and abnormal cell migration is involved in life-threatening diseases, including vascular disease, tumor formation, and metastasis. The advances in hydrogel-based 3D cell culture development facilitated the investigation of cell motility behavior, including cell-cell and cell-matrix adhesion and cell migration in a microenvironment more related to in vivo situations. Establishing advanced methods for these in vitro studies is thus necessary. Photo-sensitive proteins show advantages in remote and non-invasive regulation of hydrogels’ properties, and thus are of great potential in regulating 3D cultured cells’ behavior. In the presented study, we engineered photocleavable protein(PhoCl)-decorated hydrogels to regulate cell adhesion and migration of MDA-MB-231. The integrin-binding motif RGD was fused to the PhoCl and was decorated on the hydrogel. After being exposed to light at 405 nm, the PhoCl was cleaved and the RGD motif was released, resulting in detachment of the binding cells. The regulatory effect of the light illumination showed a time-dependent and cell density-dependent manner. Furthermore, the elimination of RGD by patterned light exposure completely suspended the cell migration to the corresponding region, suggesting a controllable regulation of the cell migration direction.

关键词： Cell adhesion Cell migration Photoresponsive protein Hydrogel Photoresponsive hydrogel photocleavable protein

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