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Characterization of Two Putative Protein Phosphatase Genes and Their Involvement in phosphorus efficiency in Phaseolus vulgaris

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Journal of Integrative Plant Biology 2012年第6期54卷 400-411页

作者： Cui-Yue Liang Zhi-Jian Chen Zhu-Fang Yao Jiang Tian Hong Liao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Root Biology CenterSouth China Agricultura UniversityGuangzhou 510642China Robert Holley Center for Agriculture and Health United States Department of AgricultureAgricultural Research ServiceCornell UniversityIthacaNew York 14853USA

Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2：1 and PvPS2：2 were identified and characterized in bean （Phaseolus vulgaris）. The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2：GFP in onion epidermal cells. Transcripts of the two PvPS2 genes were significantly increased by phosphate （P1） starvation in the two bean genotypes, G19833 （a P-efficient genotype） and DOR364 （a P-inefficient genotype）. However, G19833 exhibited higher PvPS2：1 expression levels than DOR364 in both leaves and roots during P1 starvation. Increased transcription of PvPS2：1 in response to P1 starvation was further verified through histochemical analysis of PvPS2：I promoter fusion b-glucuronidase （GUS） in transgenic Arabidopsis plants. Analysis of PvPS2：1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2：1 was involved in root growth and P accumulation. Furthermore, expression levels of two P1 starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2：1 Arabidopsis lines. Taken together, our results strongly suggested that PvPS2：1 positively regulated plant responses to P1 starvation, and could be further targeted as a candidate gene to improve crop P efficiency.

关键词： phosphorus efficiency HAD protein acid phosphatase bean.

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Screening and identification of soybean varieties with high phosphorus efficiency at seedling stage

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Oil Crop Science 2021年第1期6卷 41-49页

作者： Hui Wang Ahui Yang Guang Yang Hongyu Zhao Futi Xie Huijun Zhang Haiying Wang Xue Ao College of Agronomy Shenyang Agricultural UniversityShenyangChina

In order to simplify the workload and find a reliable method for screening soybeans with different phosphorus(P)efficiencies,47 soybean varieties were screened from 90 varieties according to yield under normal phosphorus,and 10 indicators including root phenotype,phosphorus utilization efficiency at the seedling stage and yield were *** single-index cluster analysis,the performance value and relative value of the abovementioned indexes under low-phosphorus conditions were analyzed,and then,the combined indexes were analyzed by principal components *** membership function method and the cluster analysis method was used to calculate and analyze the comprehensive score *** indexes of root P utilization efficiency(X_(1)),relative value of root P utilization efficiency(X_(2)),and root volume(X_(3))under low P at the seedling stage were selected as the most consistent with the yield screening results under low P condition,and the mathematical model of the comprehensive score value was obtained:D=1.218X_(1)t 0.320X_(2)t 0.007X_(3)-0.664(P=0.000,R^(2)=1.000).The comprehensive score can screen and identify the P efficiency of soybeans with fewer indexes in the early growth stages,which provides a more rapid and reliable mathematical model for screening and identifying a larger number of germplasm resources for P efficiency.

关键词： Soybean Low phosphorus stress Seedling stage phosphorus efficiency

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Genotypic Variation in Root Morphological Characteristics of Common Bean in Relation to phosphorus efficiency

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Acta Botanica Sinica 2001年第11期43卷 1161-1166页

作者：廖红严小龙华南农业大学植物营养遗传研究室根系生物学研究中心广州510642

Root morphology in plants may be related to phosphorus (P) efficiency by affecting the absorption characteristics of the root system. However, genotypic variation in root morphological characteristics of common bean ( Phaseolus vulgaris L.) as affected by P availability has not been well clarified. In the present study, systematic studies were conducted in a P-buffered sand culture system using three pairs of common bean parental materials with contrasting root traits in response to P deficiency. The results indicate that P availability significantly affects bean root morphology. Common bean tends to have smaller root system, shorter and coarser roots at low P availability. Genotypic variation in root morphology was observed among different genotypes in response to P availability. The P efficient genotypes appear to have larger, finer and longer root systems than the P inefficient genotypes, and such a variation was particularly obvious in the basal roots. From allomeric analysis, we found that morphological characteristics of the basal roots contribute more to P efficiency than those of the tap roots. Further studies with the F-9 recombinant inbred lines derived from one of the most contrasting parental pairs, DOR364 and G19833, confirmed the above findings, indicating that those morphological characteristics are inheritable hence provide potential for genetic improvement.

关键词： common bean root morphology phosphorus efficiency genotypic variation

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Phylogeography and Characteristics of phosphorus efficiency of Secondary Core Collection for Rice Landraces in Yunnan Province

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Agricultural Science & Technology 2011年第4期12卷 592-596页

作者： ZENG Ya-wen DU Juan PU Xiao-ying YANG Shu-ming YANG Tao WEI Ming-liang ZHANG Hao ZHOU Guo-hua Key Laboratory of Yunnan Academy of Agricultural Sciences/Agricultural Biotechnology Institute of Biotechnology and Genetic Germ- plasm Kunming 650205 Yunnan Agricultural University Kunming 650201 Kuming Tiankang Science and Technology Limited Company Kunming 650231 Lijiang Academy of Agricultural Sciences Lijiang 674113

[Objective] The paper was to explore the causes of phosphorus(P)efficiency of rice in Yunnan Province,and to provide strategy for ecological and environmental protection.[Method]Using 703 accessions of secondary core collections from 16 prefectures of five regions in Yunnan Province,two treatments of low available P(6.26mg/kg)versus normal P(available P 40mg/kg),and invalid P(available P 0.02 mg/kg)versus normal P(available P 70 mg/kg)were set,and zonal characteristics of phosphorus efficiency and the activation characteristics of invalid P of rice landraces were investigated.[Result] phosphorus efficiency and the activation characteristics of invalid P in soils from rice landraces of Yunnan Province had the similar identification indexes,*** relative indexes of four traits(tillering ability or effective panicle,root weight,biomass,and straw weight)could be the screening indexes of gene type of secondary core collection with phosphorus efficiency,which also reflected the zonal characteristics of phosphorus efficiency and the activation characteristics of invalid P in soils of 16 prefectures among five *** the contrary,the relative panicle length,node length under panicle,leaf length,leaf width and plant height could only be the assistant indexes of identification for phosphorus *** were similar zonal characteristics between phosphorus efficiency and the activation characteristics of invalid P in soils of rice landraces in Yunnan Province.[Conclusion] The study had great importance to the conservation and utilization of biological diversity,which would make contribution to second green revolution of "less input,multiple output,promote health,and protect the environment".

关键词： phosphorus efficiency Phylogeography Characteristics Secondary core collection Rice landraces

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Brassica napus Root Mutants Insensitive to Exogenous 6-BA Show phosphorus efficiency

Brassica napus Root Mutants Insensitive to Exogenous 6-BA Sh...

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第十二届全国植物基因组学大会

作者： Taoxiong Shi,Dongyue Zhao,Fangsen Xu,Jinling Meng,Lei Shi~* National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China

Cytokinins are known to negatively regulate plant root development and phosphorus(P) starvation responses. In this study,two Brassica napus root mutants insensitive to exogenous 6-BA,Bnprl1(longer primary root) and Bnlrn1(more lateral roots),were screened and used to evaluate P efficiency in hydroponics,along with WT ***1 produced the highest shoot dry weight(SDW),root dry weight(RDW) at both low P(LP) and high P(HP).Bnprll had more SDW at both P levels and RDW at ***,Bnlrn1 accu-

关键词： Brassica napus Bnprl1 Bnlrn1 cytokinin phosphorus efficiency

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Difference in Absorption of N, P and K among Different-phosphorus efficiency Soybean Genotypes at the Seedling Stage

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Agricultural Science & Technology 2014年第12期15卷 2145-2149页

作者：张彦丽谷思玉律凤霞齐虹凌杨松鹏牡丹江师范学院生命科学与技术学院黑龙江牡丹江157011 东北农业大学资源与环境学院黑龙江哈尔滨150030

The low-phosphorus efficiency soybean genotypes, D03, D05, D17 and D18, and the high-phosphorus efficiency soybean genotypes, D31, D34, D37 and D38 were selected. The soil culture experiment was conducted. There were 2 treat- ments, high phosphorus （＋P） and low phosphorus （-P）. The difference in absorption of N, P and K among different-phosphorus efficiency soybean genotypes at the seedling stage was analyzed. The results showed in the low phosphorus （-P） treat- ment, the P absorption amount differed among roots, stems and leaves of different high-phosphorus efficiency genotypes. The P absorption amount was positively relat- ed （P〈0.05, P〈0.01） to the dry weights of root, stem and leaves, but was negative- ly related to the utilization efficiency of phosphorus （P〉0.05）. The N and K absorp- tion amounts of root, stem and leaves all trended to decrease. The low phosphorus stress would inhibit the absorption of N, P and K in soybean plants at the seedling stage. However, the adaptability of the high-phosphorus efficiency soybean geno- types to low phosphorus stress was significantly higher than that of low-phosphorus efficiency genotypes.

关键词： Soybean Seedling stage phosphorus efficiency phosphorus stress

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Analysis of phosphorus Nutrition efficiency of Soybean Genotypes with Different phosphorus efficiency at Blooming Stage under Low phosphorus Stress

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Agricultural Science & Technology 2012年第12期13卷 2544-2548页

作者：张彦丽牡丹江师范学院生命科学与技术学院黑龙江牡丹江157011

[Objective] This study aimed to investigate the phosphorus nutrition effi- ciency of different soybean genotypes at blooming stage under low phosphorus stress. [Method] By using four ＂low phosphorus efficiency＂ soybean genotypes （D03, D05, D17 and D18） and four ＂high phosphorus efficiency＂ soybean genotypes （D31, D34, D37 and D38） as experimental materials, soil culture experiment was conduct- ed with two treatments of high phosphorus （＋P） and low phosphorus （-P）, to analyze the relationship between phosphorus content, phosphorus uptake, phosphorus use efficiency and phosphorus efficiency of soybean genotypes with different phos- phorus efficiency. [Result] Under low phosphorus conditions, four soybean genotypes with high phosphorus efficiency showed significant advantage in phosphorus uptake at seedling stage, to be specific, D34 showed relatively high phosphorus absorption capacity but no advantage in adaptability of phosphorus use efficiency, while only D37 showed relatively high phosphorus absorption capacity and phosphorus use ca- pacity. Correlation analysis and path analysis showed that the level of phosphorus efficiency of soybean at blooming stage under （-P） and （＋P） treatments was mainly determined by phosphorus absorption capacity, and that under （-P） treatment was significantly greater than （＋P） treatment. phosphorus uptake and phosphorus use ef- ficiency under （-P） and （＋P） treatments both showed great direct effects on phos- phorus efficiency, and phosphorus uptake made greater contribution; however, the indirect effects were relatively low, Under low phosphorus stress, the mechanisms for various soybean genotypes with high phosphorus efficiency to adapt to low phospho- rus stress were different, and phosphorus absorption efficiency （phosphorus uptake） was the main variation source of the phosphorus efficiency of various soybean genotypes at blooming stage. [Conclusion] This study revealed the contribution made by phosphorus absorption efficiency and p

关键词： Soybean Low phosphorus stress phosphorus efficiency Absorption ef- ficiency Use efficiency

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Differences in phosphorus Absorption and Utilization efficiency of Soybean in Mature Period under phosphorus Stress

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Agricultural Biotechnology 2014年第6期3卷 24-27,30页

作者： Yanli ZHANG Hongling QI Fengxia LU Ming HAN Panpan WANG College of Life Science and Technology Mudanjiang Normal University

In this study, four phosphorus-inefficient soybean genotypes （1903, 1305, D17 and D18） and four phosphorus-efficient soybean genotypes （D31, D34, D37 and D38 ） were selected as experimental materials for soil culture experiment under high and low phosphorus treatments, to investigate the grain yield, phosphorus content, phosphorus uptake and the relationship between phosphorus utilization efficiency and phosphorus efficiency of soybean genotypes with different phos- phorus efficiency in mature period. According to the experimental results, under low phosphorus treatment, four phosphorus-efficient soybean genotypes exhibited significantly high phosphorus uptake in mature period, especially for D31 and D37; however, three phosphorus-efficient genotypes showed no advantages in adapta- bility of phosphorus utilization efficiency, while only I）31 exhibited high phosphorus utilization and absorption capacity. Correlation analysis and path analysis showed that phosphorus deficiency of soybean was mainly detemained by phosphorus absorption capacity, and phosphorus deficiency under （-P） treatment was sig- nificandy higher than （＋ P） treatment. phosphorus uptake and phosphorus utilization efficiency posed great direct effects on phosphorus efficiency, and phosphorus uptake exhibited a greater contribution ; in addition, these two factors both posed small indirect effects. In mature period at reproductive growth stage, phosphorus absorption efficiency （phosphorus uptake） was the main variation source of phosphorus efficiency of various soybean genotypes in mature period. Therefore, strong phosphorus uptake and accumulation capacity of phosphorus-efficient soybean genotypes in mature period is an important nutrition foundation for the information of high grain yield.

关键词： Soybean Low phosphorus stress phosphorus efficiency Absorption efficiency Utilization efficiency

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Genome-Wide Expression Profile of Maize Root Response to phosphorus Deficiency Revealed by Deep Sequencing

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Journal of Integrative Agriculture 2014年第6期13卷 1216-1229页

作者： SU Shun-zhong WU Ling LIU Dan LU Yan-li LIN Hai-jian ZHANG Shu-zhi SHEN Ya-ou LIU Hai-lan ZHANG Zhi-ming RONG Ting-zhao ZHANG Xiao TIAN Yue-hui NIE Zhi GAO Shi-bin Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of Agriculture/Maize Research InstituteSichuan Agricultural University

phosphorus （P） is one of the three primary macronutrients that are required in large amounts for plant growth and development. To better understand molecular mechanism of maize and identify relevant genes in response to phosphorus deficiency, we used Solexa/Illumina＇s digital gene expression （DGE） technology to investigate six genome-wide expression profiles of seedling roots of the low-P tolerant maize inbred line 178. DGE studies were conducted at 6, 24 and 72 h under both phosphorus deficient and sufficient conditions. Approximately 3.93 million raw reads for each sample were sequenced and 6 816 genes exhibited significant levels of differential expressions in at least one of three time points in response to P starvation. The number of genes with increased expression increased over time from 6 to 24 h, whereas genes with decreased expression were more abundant at 72 h, suggesting a gradual response process for P deficiency at different stages. Gene annotations illustrated that most of differentially expressed genes （DEGs） are involved in different cellular and molecular processes such as environmental adaptation and carbohydrate metabolism. The expression of some known genes identified in other plants, such as those involved in root architecture, P metabolism and transport were found to be altered at least two folds, indicating that the mechanisms of molecular and morphological adaptation to P starvation are conserved in plants. This study provides insight into the general molecular mechanisms underlying plant adaptation to low-P stress and thus may facilitate molecular breeding for improving P utilization in maize.

关键词： maize phosphorus efficiency root digital gene expression

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phosphorus Uptake and Utilisation Efficiencies of Different Wheat Cultivars Based on a Sand-Culture Screening System

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Pedosphere 2002年第4期12卷 329-337页

作者： N.K.HOWES S.E.SMITH ZHUYONGGUAN DepartmentofSoilandWater TheUniversityofAdelaideGlenOsmondSA5064（Australia） ResearchCentreforEco－EnvironmentalSciences theChineseAcademyofSciencesBeijing100085（China） SouthAustraliaResearchandDevelopmentInstitute GlenOsmondSA5064（Australia）

A sand-based culture system using rock phosphate (P) was developed to simulate the situation in alkalinesoils, with respect to the dominant P form, and five wheat cultivars (Excalibur, Brookton, Krichauff, Westoniaand Sunco) were tested in this screening system to compare their P uptake and utilisation efficiencies. Resultsshowed that these cultivars differed significantly in their ability to acquire P from the sparingly available form(rock phosphate in this case). The accumulation of P by Brookton was three times that by Krichauff. Pconcentrations in plant tissues did not differ significantly, indicating that all cultivars were similar in Putilisation efficiency. A further experiment showed that the greater ability of a cultivar to take up P fromsparingly available form was related to the ability of a cultivar to acidify the rhizosphere. Seed P content wasa confounding factor in this system, and the use of relatively uniform seed with similar P content, preferablylow, was conducive to a successful outcome of the screening process.

关键词： phosphorus efficiency sand-culture uptake wheat genotypes

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