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Isolation of phloridzin-degrading,IAA-producing bacterium Ochrobactrum haematophilum and its effects on the apple replant soil environment

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Horticultural Plant Journal 2023年第2期9卷 199-208页

作者： Weitao Jiang Ran Chen Lei Zhao Yanan Duan Haiyan Wang Zhubing Yan Xiang Shen Xuesen Chen Chengmiao Yin Zhiquan Mao State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandongChina

We isolated and identified a bacterium that could produce IAA and degrade phloridzin in the rhizosphere soil of healthy replanted apple(the rootstock is M9T337 and the scion is Yanfu 3),providing a theoretical basis for reducing the obstacles associated with apple replant disease(ARD).Isolates were screened using Salkowski colorimetry and screening medium for *** isolate of interest(W6)was identified as Ochrobactrum haematophilum based on morphological analysis,physiological and biochemical tests,and 16S rDNA *** a laboratory experiment,W6 produced auxin and promoted the growth of Arabidopsis thaliana roots,and its degradation rate of 100 mg.L^(-1 )phloridzin was 62.0%.In a pot experiment,W6 significantly reduced the phenolic acid contents of replanted soil,lowered the abundance of the harmful fungus Fusarium solani,and increased soil enzyme activities,thereby improving the micro-ecological environment of replant soil.W6 increased the root antioxidant enzyme activity and leaf photosynthetic pigment content of replanted Malus hupehensis ***,effectively alleviating the decrease in net photosynthetic rate,transpiration rate and stomatal conductance caused by *** a field experiment,W6 also promoted the growth of replanted apple(the rootstock is M9T337 and the scion is Yanfu 3)***,W6 can promote apple growth and degrade phenolic acids,and it can be used as an effective treatment for the reduction of ARD.

关键词： Malus hupehensis Rehd. Apple Apple replant disease Ochrobactrum haematophilum phloridzin Rhizosphere soil

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Estrogenic and Antiestrogenic Activities of phloridzin

Estrogenic and Antiestrogenic Activities of phloridzin

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中国药理学会第十次全国学术会议

作者： CHNG Mi-hwa Masao HATTORI Institute of Natural Medicine University of Toyama

Our research demonstrated that phloridzin has double directional adjusting function of estrogen and antiestrogen *** has significant effects on the proliferation of estrogen sensitive ER(+) MCF-7 cells in absence of *** addition of phloridzin simultaneously,phloridzin showed antagonism on estradiol-induced MCF-7 cells proliferation,but it did not significantly affect proliferation of Estrogen insensitive ER(-) MDA-MB-231 *** inducedβ-galactosidase activity in a yeast two-hybrid *** increase of the uterine weight and serum estradiol content of mouse was observed when administered orally for 7 *** oral administration, phloridzin was absorbed mainly into the blood,a small part is metabolized to *** study proved that phloridzin can be distributed to the target organ and play the role of phytoestrogen.

关键词： Malus hupehensis phloridzin MCF-7 MDA-MB-231 Yeast two-hybrid assay phytoestrogen

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Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f.

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Asian Pacific Journal of Tropical Biomedicine 2021年第2期11卷 59-65页

作者： Shanti Vasudevan C.N. Bibu John Kariyil Athira Nair D. I’ma Neerakkal Department of Botany Sacred Heart CollegeThevaraKochi 682013KeralaIndia Department of Veterinary Pharmacology&Toxicology College of Veterinary and Animal SciencesPookode 673576WayanadKeralaIndia

Objective:To evaluate the anti-inflammatory potential of aqueous extract of Pterocarpus santalinus *** using molecular docking and in vivo ***:An aqueous extract of Pterocarpus santalinus heartwood was prepared using a Soxhlet *** in the extract were tentatively identified using high-resolution mass *** docking experiments were carried out to evaluate the binding affinity of selected compounds,phloridzin to cyclooxygenase-1(COX-1),cyclooxygenase-2(COX-2),prostaglandin E synthase-1(PGES-1)and 5-lipoxygenase(5-LOX).Anti-inflammatory potential was evaluated by carageenan induced paw edema model in ***:The presence of major component phloridzin along with quercetin,parthenin,ginkgolide B,picrotoxinin,usnic acid,octopine,and epigallocatechin was detected in the *** docking study showed that phloridzin inhibited COX-1,COX-2,PGES-1 and 5-LOX with more affinity than ibuprofen and *** santalinus heartwood extract at 200 and 400 mg/kg BW showed significant reduction in carageenan-induced hind paw edema in a dose-dependent manner,but the effect was slow when compared with the standard ibuprofen(30 mg/kg p.o.).Conclusions:The study indicated that after clinical trials,the aqueous extract of Pterocarpus santalinus heartwood can be effectively used in phytotherapy to treat inflammation.

关键词： phloridzin COX-1 COX-2 PGES-1 5-LOX

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Assessing bioaccessibility and bioavailability in vitro of phenolic compounds from freeze-dried apple pomace by LC-Q-TOF-MS

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Food Bioscience 2022年第4期48卷 733-740页

作者： Luna Pollini Ana Juan-García Francesca Blasi Jordi Manes Lina Cossignani Cristina Juan Department of Pharmaceutical Sciences Food Science and Nutrition SectionUniversity of PerugiaItaly Laboratory of Food Chemistry and Toxicology Faculty of PharmacyUniversity of ValenciaSpain

Apple pomace is one of the most abundant by-product in fruit processing *** this work,fresh and freeze-dried apple pomace variety‘Red Delicious’apples“*** de Girona”was used to evaluate the potential amount of phenolic compounds available from the food matrix by in vitro bioaccessibility *** the simulated digestion of freeze-dried apple pomace,an in vitro bioavailability test by using differentiated Caco-2 cells simulating the intestinal barried were assayed for 1 h,2 h,3 h and,4 ***-Q-TOF-MS has permitted to perform a monitoring of phloridzin,chlorogenic and gallic acids in apple pomace in apical and basal *** obtained with LC-Q-TOF-MS demonstrated that chlorogenic acid had greater percentage of bioaccesibility(44%),than phloridzin(17%)and gallic acid(7%),with similar values for fresh and freeze-dried ***,only chlorogenic acid reported higher bioavailability in basal compartments after 3 h and 4 h,in particular about 56%of chlorogenic acid passed the transepithelial *** is the first work about the evaluation of bioavailability of polyphenols from digested freeze-dried apple pomace ***,this interesting by-product rich in polyphenol,with a great bioavailability,should be a promising ingredient to develop new functional foods.

关键词： Apple pomace Bioaccessibility Bioavailability Chlorogenic acid phloridzin Caco-2 cells

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齿叶黄杞化学成分研究(Ⅰ)

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中国药物化学杂志 2001年第1期11卷 51-52页

作者：刘宏伟姚新生沈阳药科大学中药系辽宁沈阳110016

从齿叶黄杞 (EngelhardtiaserrataBl.)茎皮中分离得到 4个化合物 ,经过理化性质和波谱分析鉴定为 :没食子酸 (1)、phlorizin(2 )、(- )儿茶素 (3)、β 谷甾醇 (4 ) 。

关键词：齿叶黄杞没食子酸 phloridzin (一)榈儿茶素 β

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