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Molecular and Biochemical Evidence for phenylpropanoid Synthesis and Presence of Wall-linked Phenolics in Cotton Fibers

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Journal of Integrative Plant Biology 2009年第7期51卷 626-637页

作者： Ling Fan Wei-Jun Shi Wen-Ran Hu Xinn-Ynn Hao Dong-Mei Wang Hui Yuan Hong-Ying Yan Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences Urumqi 830091 China Institute of Economic Crops Xinjiang Academy of Agricultural Sciences Urumqi 830091 China

The mature cotton （Gossypium hirsutum L.） fiber is a single cell with a typically thickened secondary cell wall. The aim of this research was to use molecular, spectroscopic and chemical techniques to investigate the possible occurrence of previously overlooked accumulation of phenolics during secondary cell wall formation in cotton fibers. Relative quantitative reverse transcription-polymerase chain reaction analysis showed that GhCAD6 and GhCAD1 were predominantly expressed among seven gene homologs, only GhCAD6 was up-regulated during secondary wall formation in cotton fibers. Phylogenic analysis revealed that GhCAD6 belonged to Class I and was proposed to have a major role in monolignol biosynthesis, and GhCAD1 belonged to Class III and was proposed to have a compensatory mechanism for monolignol biosynthesis. Amino acid sequence comparison showed that the cofactor binding sites of GhCADs were highly conserved with high similarity and identity to bona fide cinnamyl alcohol dehydrogenases. The substrate binding site of GhCAD1 is different from GhCAD6. This difference was confirmed by the different catalytic activities observed with the enzymes. Cell wall auto-fluorescence, Fourier transform infrared spectroscopy （FTIR）, high-performance liquid chromatography （HPLC） and chemical analyses confirmed that phenolic compounds were bound to the cell walls of mature cotton fibers. Our findings may suggest a potential for genetic manipulation of cotton fiber properties, which are of central importance to agricultural, cotton processing and textile industries.

关键词： cell wall cotton fiber gene expression phenolics phenylpropanoid biosynthesis pathway

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Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang

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Food Science and Human Wellness 2022年第4期11卷 854-864页

作者： Hao Wen Wenqiang Wang Xi Jiang Minyu Wu Hongjin Bai Cuiyun Wu Lirong Shen Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China College of Horticulture Forestry Tarim University of Land-ReclamationAral 843300China

Korla fragrant pear(KFP)with special fragrance is a unique cultivar in Xinjiang,*** order to explore the biosynthesis molecular mechanism of chlorogenic acid(CGA)in KFP,the samples at different development periods were collected for transcriptome *** performance liquid chromatography analysis showed that CGA contents of KFP at 88,118 and 163 days after full bloom were(20.96±1.84),(12.01±0.91)and(7.16±0.41)mg/100 g,respectively,and decreased with the fruit *** from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean ***‘phenylpropanoid biosynthesis’pathway including 57 unigenes,11 PALs,1 PTAL,64CLs,9 C4Hs,25 HCTs and 5 C3’Hs related to CGA biosynthesis was *** was found that the expression levels of 11 differentially expressed genes including 1 PAL,2 C4Hs,34CLs and 5 HCTs were consistent with the change of CGA *** polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq *** findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.

关键词： Korla fragrant pear Development period Chlorogenic acid content De novo transcriptome assembly phenylpropanoid biosynthesis pathway

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