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Metabolic profile analysis of free amino acids in experimental autoimmune uveoretinitis rat plasma

International Journal of Ophthalmology(English edition) 2019年第1期12卷 16-24页

作者： Jun-Guo Guo Xin-Miao Guo Xing-Rong Wang Jing-Zhen Tian Hong-Sheng Bi College of Pharmacy Shandong University of Traditional Chinese Medicine Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases Eye Institute Shandong University of Traditional Chinese Medicine School of Management Shandong University of Traditional Chinese Medicine Affiliated Eye Hospital Shandong University of Traditional Chinese Medicine

AIM: To determine the differences of amino acid(AA) levels in experimental autoimmune uveoretinitis(EAU). METHODS: AA analysis of the plasma samples in EAU rats induced by interphotoreceptor retinoid-binding protein emulsion were performed with high performance liquid chromatography(HPLC) and phenylisothiocyanate(PITC) pre-column derivation methods were performed. Using partial least squares discriminant analysis(PLS-DA), the potential biomarkers were identified in EAU rat plasma, and the metabolic pathways related to EAU were further analyzed. RESULTS: The method results showed that linear(r≥0.9957), intra-day reproducible [relative standard deviation(RSD)=0.04%-1.33%], inter-day reproducible(RSD=0.06%-2.07%), repeatability(RSD=0.03%-0.89%), stability(RSD=0.05%-2.48%) and recovery(RSD=1.98%-4.39%), with detection limits of 0.853-11.4 ng/mL. The metabolic profile in EAU rats was different from that in the control groups five AAs concentrations were increased and nine AAs were reduced. Moreover, five metabolic pathways were related to the development of EAU. CONCLUSION: The developed method is a simple, rapid and convenient for determination of AAs in EAU rat plasma, and these findings will provide a comprehensiveinsight on the metabolic profiling of the pathological changes in EAU.

关键词： experimental autoimmune uveoretinitis phenylisothiocyanate high performance liquid chromatography amino acid metabolic profile rat

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Synthesis of Some New Arylazothiophene and Arylazopyrazole Derivatives as Antitumor Agents

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Pharmacology & Pharmacy 2012年第2期3卷 148-157页

作者： Ahmed Ali Fadda E. Abdel-Latif Rasha E. El-Mekawy Chemistry Department Faculty of Science Mansoura University Mansoura Egypt Chemistry Department Faculty of Science Mansoura University Mansoura Egypt.

The starting 1-phenylbutane-1,3-dione (1) was used as key intermediate for the synthesis of several new thiophene and pyrazole derivatives. The newly synthesized compounds were evaluated for in vitro cytotoxicity against an Ehrlich ascites cells and in vivo cytotoxicity for compound 10d using EAC assay and 5-fluorouracil is used as reference drug. Compounds 7c, e and 10c, d showed significant activity in certain cancer cell and have been targeted for further studies, compound 10d is more effective and showed the highest activity. Structures of the newly prepared compounds were confirmed by both spectral, analytical data and molecular calculations.

关键词： Benzoylacetone phenylisothiocyanate Thiophene Pyrazole Thiocarbamoyl Cytotoxic Agents

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