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Effect of tehina processing and storage in the physical-chemical quality

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International Journal of Agricultural and Biological Engineering 2016年第5期9卷 218-226页

作者： Taha M.Rababah Muhammad Al-U’datt Majdi Al-Mahasneh Mohammad Obaidat Ali Almajwal Aladdin Odeh Susan Brewer Wade Yang Faculty of Agriculture Jordan University of Science and TechnologyIrbid 22110Jordan Faculty of Engineering Jordan University of Science and TechnologyIrbid 22110Jordan Faculty of Veterinary Medicine Jordan University of Science and TechnologyIrbid 22110Jordan Department of Community Health Sciences College of Applied Medical SciencesKing Saud UniversityRiyadh 11451Saudi Arabia Department of Food Science and Human Nutrition University of Illinois at Urbana-ChampaignUrbanaIL 6180USA Department of Food Science and Human Nutrition University of FloridaGainesvilleFL 32611-0370USA

The effect of tehina processing and storage during six months at 25℃and 35℃on total phenolics,antioxidant activity,anthocyanins,and fatty acid analysis was evaluated.Tehina processing,storage period and temperature significantly(p≤0.05)decreased total phenolics,antioxidant activity and anthocyanins contents.The results showed that raw sesame seeds had the highest total phenolics(99.98 mg GAE/100 g)and lower levels were found in dehulled unroasted sesame seeds,tehina at zero time and roasted sesame seeds(62.53,60.61 and 47.03 mg GAE/100 g,respectively).In addition,tehina during storage showed a significant decrease in total phenolics(60.61 to 42.45 mg GAE/100 g).Raw sesame seeds had the highest antioxidant activity(0.51 mg/mL),while the tehina,dehulled unroasted and roasted sesame had antioxidant activity values of 1.21,1.35 and 1.79 mg/mL,respectively.Also,antioxidant activity of tehina at zero time was highest(1.21 mg/mL)and lowest was in tehina stored for six months(1.70 mg/mL).Sesame seeds and tehina had low levels of anthocyanins(1.26 and 0.50 mg cya-3-glu/100 g).Fatty acid analysis showed that sesame seeds(raw,dehulled unroasted and roasted)and tehina samples contain high percent of unsaturated fatty acid oleic(C18:1)and linoleic(C18:2).

关键词： sesame seeds phenols antioxidant activities anthocyanins

Fractions of a chloroform extract of ajenjo leaves (Artemisiamendozana DC. var. mendozana) inhibit the proliferation,viability and clonogenicity of B16-F0 melanoma cells

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BIOCELL 2020年第3期44卷 293-299页

作者： MARÍA ELISA MILLÁN MARÍA FERNANDA MARRA LEONARDO ANDRÉS SALVARREDI EMILIO FERNANDO LIZARRAGA LUIS ALBERTO LOPEZ Universidad Nacional de Cuyo MendozaArgentina Comisión Nacional de Energía Atómica Buenos AiresArgentina Fundación Escuela de Medicina Nuclear MendozaArgentina Fundación Miguel Lillo y Universidad Nacional de Tucumán San Miguel de TucumánArgentina

The ajenjo,Artemisia mendozana DC.var.mendozana(Asteraceae),grows in the Andean foothills of Mendoza and San Juan,Argentina,and is used as a medicinal plant for its antispasmodic and antifungal properties.The aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation,viability and clonogenicity of B16-F0 melanoma cells.Using a silica gel chromatography column,120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions(F1–F9).Their major compounds identified were:terpenes(F1),terpenes and sesquiterpene lactones(F2–F3),sesquiterpenes(F4–F6)and phenols and sesquiterpenes(F7-9).B16-F0 cells were incubated for 72 h with DMSO(vehicle)or 0.1 mg/ml F1–F9.At 72 h of culture,F1 decreased both the growing index(GI)and cell viability.F2 and F3 both decreased GI and only F3 decreased clonogenic activity.F4 and F5 both decreased GI.Only F5 decreased cell viability and F4 decreased clonogenicity.Consequently,fractions F6–F8 did not affect any of the cell parameters assayed,while F9 decreased cell viability and inhibited clonogenicity.

关键词： Terpenes Sesquiterpene lactones Sesquiterpenes phenols

Total phenolic content and antioxidant activity of six wild Mentha species(Lamiaceae) from northeast of Algeria

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 760-766页

作者： Amina Benabdallah Chaabane Rahmoune Mahieddine Boumendjel Oumayma Aissi Chokri Messaoud Department of Biology and Ecology Laboratory of Ecotoxicology and Abiotic StressUniversity Frères Mentouri Constantine 1 Department of Agronomy Laboratory on Biodiversity and Ecosystems PollutionFaculty of Nature and Life SciencesUniversity Chadli Bendjedid El-Tarf Department of Biology Laboratory of Plant BiotechnologyNational Institute of Applied Science and Technology(INSAT)

Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha piperita,Mentha pulegium,Mentha rotundifolia and Mentha villosa.Methods:The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method.To evaluate tannins content,vanillin and HCl were added to methanolic extracts.The antioxidant potential was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging,ferrous ion chelating and the inhibition of b-carotene bleaching assays.Results:The methanol extracts of Algerian mints were rich in phenolic compounds and exhibited powerful antioxidant activity ranging from 7.5 mg/mL to 44.66 mg/mL,which varied significantly among species.Mentha aquatica stood out with efficient antioxidant ability which was correlated to the high total phenolics content,followed by Mentha arvensis and Mentha piperita with very close values,comparing to Mentha pulegium,Mentha rotundifolia and Mentha villosa with lowest values.Conclusions:These results show that methanolic extracts of Mentha species from Algeria have a great potential of polyphenols which can be used as a natural food preservative and antioxidant source.

关键词： Mentha phenols Flavonoids Tannins Antioxidant activity

An Approach to Determination of Phenolic Compounds in Seawater using SPME-GC-MS Based on SWCNTs Coating

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Journal of Ocean University of China 2016年第4期15卷 655-659页

作者： ZHU Jia WANG Ying ZENG Lin School of Geographic and Oceanographic Sciences Nanjing University Nanjing 210008 P. R. China Third Institute of Oceanography SOA Xiamen 361005 P. R. China

Phenolic compounds have become one kind of the important pollutants of the marine environment. Single-walled Carbon nanotubes, as one-dimensional nano materials, have light weight and perfect hexagonal structure of connections, with many unusual mechanical, chemical and electrical properties. In recent years, with the research of carbon nanotubes and other nano materials, the application prospect is also constantly discussed. In this paper, homemade single-walled carbon nanotubes(SWCNTs) coating was used for establishing an analytical approach to the determination of five kinds of phenolic compounds in seawater using SPME-GC-MS. Optimal conditions: After saturation was conducted with Na Cl, and p H was adjusted to 2.0 with H_2SO_4, the extract was immersed in a water bath at 40 for GC℃-MS determination through 40-min agitating extraction at 500 rmin^(-1) and 3-min desorption at 280℃. The liniearities ranged between 0.01-100 μg L^(-1), and the determination limits ranged between 1.5-10 ngL^(-1). The relative standard deviation(RSD, n = 5) was less than 6.5%. For the phenolic compounds obtained from the spiked recovery test for actual seawater samples, the rates of recovery were 87.5%-101.7%, and the RSDs were less than 8.8%, which met the requirements of determination. Due to its simplicity, high efficiency and low consumption, this approach is suitable for the analysis of trace amounts of phenolic compounds in marine waters.

关键词： single-walled carbon nanotubes SPME GC-MS seawater phenols

Protection of cadmium chloride induced DNA damage by Lamiaceae plants

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Asian Pacific Journal of Tropical Biomedicine 2011年第5期1卷 391-394页

作者： Ramaraj Thirugnanasampandan Rajarajeswaran Jayakumar PG and Research Department of Biotechnology Kongunadu ArtsScience CollegeGN Mills Department of Zoology Bharathiar University

Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica(A.malabarica),Leucas aspera(L.aspera)and Ocimum basilicum(0.basilicum).Methods:The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants.To determine the DNA protecting activity,various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells.The pretreated cells were exposed to H_2O_2to induce DNA damage through oxidative stress.Comet assay was done and the tail length of individual comets was measured.Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.Results:Among the three plant extracts,the highest amount of total phenolic content was found in O.basilicum(189.33 mg/g),whereas A.malabarica showed high levels of flavonoids(10.66 mg/g).O.basilicum also showed high levels of DNA protecting(85%)and radical scavenging activity.Conclusions:The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage.

关键词： Cadmium chloride DNA damage Lamiaceae plants phenols Comet assay Antioxidant activity

Foliar Application of Benzothiadiazole and Salicylic Acid to Combat Sheath Blight Disease of Rice

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Rice science 2013年第5期20卷 349-355页

作者： Neerja SOOD B.S.SOHAL J.S.LORE Department of Biochemistry Punjab Agricultural UniversityLudhiana-141004 India Department of Plant Breeding and Genetics Punjab Agricultural UniversityLudhiana-141004 India

A field study was undertaken to ascertain the effects of elicitors viz. benzothiadiazole （BTH） and salicylic acid （SA） on defense related enzymes viz. peroxidase, phenylalanine ammonia lyase, superoxide dismutase, chitinase and 13-1,3-glucanase, and phenols in rice （Pusa Basmati I） plants. First foliar spray of BTH （50 mg/kg） and SA （50 mg/kg） was done at the maximum tillering stage and inoculation with Rhizoctonia solani was carried 24 h after elicitor treatment. Elicitors were further sprayed at every growth stage. Time course analysis showed peak accumulation of defense related enzymes and phenols in the rice leaves treated with BTH and SA, and accumulation was the highest at the flowering stage. Higher enzymatic activity was observed in elicitor treated plants inoculated with R. solani. Compared to the untreated control plants, application of elicitors before R. solani inoculation significantly elicited the defense related enzymes and phenols. Moreover, application of elicitors had a positive effect on yield and disease reduction. It is suggested that pretreatment of rice leaves with BTH and SA could be used to enhance the level of protection against sheath blight and to improve rice yield in the fields.

关键词： Oryza sativa benzothiadiazole salicylic acid defense related enzyme phenols sheath blight foliar application

Purification of ice structuring protein complexes from winter wheat using Triton X-114 phase partitioning

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Frontiers of Chemical Science and Engineering 2009年第4期3卷 383-385页

作者： Huaneng XU Haiying CHEN Weining HUANG State Key Laboratory of Food Science and Technology School of Food Science and TechnologyJiangnan University1800 Lihu AvenueWuxi 214122China

Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114.The highly reactive phenols were removed,and the ISP complexes remained in the aqueous phase after phase separation.The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method.The phase separation technique provides a simple and mild way for removing phenols from ISP complexes.

关键词： ice structuring protein Triton X-114 purification extraction phenols

Tannin inhibits HIV-1 entry by targeting gp41

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Acta Pharmacologica Sinica 2004年第2期 87-92页

作者： Lin Lü2, Shu-wen LIU2,3, Shi-bo JIANG3, Shu-guang WU2,4 2Institute of Pharmaceutical Sciences of the First Medical University of PLA, Key Lab of GD for Screening of New Drug, Guangzhou, Guangdong 510515, China Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA

AIM: To investigate the mechanism by which tannin inhibits HIV-1 entry into target cells. METHODS: The inhibitory activity of tannin on HIV-1 replication and entry was detected by p24 production and HIV-1-mediated cell fusion, respectively. The inhibitory activity on the gp41 six-helix bundle formation was determined by an improved sandwich ELISA. RESULTS: Tannins from different sources showed potent inhibitory activity on HIV-1 replication, HIV-1-mediated cell fusion, and the gp41 six-helix bundle formation. CONCLUSION: Tannin inhibits HIV-1 entry into target cells by interfering with the gp41 six-helix bundle formation, thus blocking HIV-1 fusion with the target cell.

关键词： tannin drug dose-response relationship enzyme-linked immunosorbent assay HIV envelope pro- tein gp41 phenols plant extracts medical plants

Dynamic changes of nutrient content in herbaceous peony seed

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Oil Crop Science 2020年第2期5卷 74-79页

作者： Qimo Qi Yu Li Fanzhi Meng Guangping Xing Jilei Zhou Xianfeng Guo Forestry College Shandong Agricultural UniversityShandong Provincial Research Center of Demonstration Engineering Technology for Urban and Rural LandscapeTaianChina Forest Seedling and Flower Station in Shandong Province JinanChina

Herbaceous peony is a promising oilseed crop.‘Hangshao’and‘Fenghuang Niepan’are two cultivars with high yield and oil content In this study,seed reserved nutrient of these two genotypes were investigated during seed development process.Results showed that‘Hangshao’and‘Fenghuang Niepan’matured approximately 85 d after flowering(DAF).The development p rocess could be divided into four stages:seed formation and enlargement stage(S1),seed inclusion and enrichment stage(S2),crude fat rapid accumulation stage(S3)and dehydration and color transition stage(S4).During this process,100-seed fresh weight(HFW)and 100-seed dry weight(HDW)increased,whereas water content(WC)decreased.HFW,HDW and WC for‘Hangshao’seed at 85 DAF were28.56 g,20.78 g and 37.50%respectively,whereas those for‘Fenghuang Niepan’seed were 27.2 g,19.78 g,and30.85%respectively.In terms of the primary metabolites measuring,soluble protein content was consistently low(only 2.24%-3.15%),while starch content was relatively high(5.60%-24.81%).They displayed a trend of gradual increasing in early stage,followed by rapid decline.Crude fat tended to accumulate continuously with a linear upward trend starting at 40 DAF,and its maximum level was reached at 85 DAF(27.57%for‘Hangshao’and29.57%for‘Fenghuang Niepan’).For the secondary metabolites determination,total phenols content was relatively steady,maintaining at 107.7-129.9 mg/g for both cultivars,while total flavonoids content varied within25.33-65.33 mg/g for‘Hangshao’and 24.33-52.37 mg/g for Tenghuang Niepan’.The maximum level for both cultivars occurred at 55 DAF.Furthermore,crude fat content was found to be highly correlated with WC by correlation analysis and linear regression model definition(P≤0.01 and r=0.954).Thus,WC was recommended for evaluating crude fat in peony seed.These findings could provide scientific reference for high-yielding production practice for oleaginous peony plant.

关键词： Crude fat Flavonoids Paeonia lactiflora phenols Protein Seed development Starch