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Evaluation of Protein Extraction Methods for Vitis vinifera Leaf and Root Proteome Analysis by Two-Dimensional Electrophoresis

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Journal of Integrative Plant Biology 2010年第10期52卷 933-940页

作者： Neila Jellouli Asma Ben Salem Abdelwahed Ghorbel Hatem Ben Jouira Laboratoire de Physiologie Moléculaire de la Vigne Centre de Biotechnologie de Borj Cédria

An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis （2-DE） for recalcitrant plant species, in particular for grapevine （Vitis vinifera L.）. Trichloroacetic acid-acetone （TCA-acetone） and phenol extraction methods were evaluated for proteome analysis of leaves and roots from the Tunisian cultivar ＇Razegui＇. The phenol-based protocol proved to give a higher protein yield, a greater spot resolution, and a minimal streaking on 2-DE gels for both leaf and root tissues compared with the TCA-based protocol. Furthermore, the highest numbers of detected proteins on 2-DE gels were observed using the phenol extraction from leaves and roots as compared with TCA-acetone extraction.

关键词： PLANT proteins VITIS vinifera LEAVES ROOTS (Botany) PLANT extracts ELECTROPHORESIS SEPARATION (Technology) phenols EXTRACTION (Chemistry) GRAPES PLANT proteomics

Focuses of material science development in recent years

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Science China(Technological Sciences) 2011年第6期54卷 1645-1648页

作者： WANG Jing Science China Press 16 Donghuangchenggen North Street Beijing 100717 China

Materials science is an interdisciplinary field applying the properties of matter to various areas of science and engineering. This scientific field investigates the relationship between the structure of materials at atomic or molecular scales and their macroscopic properties. It incorporates elements of applied physics and chemistry. With significant media attention focused on nanoscience and nanotechnology in recent years, materials science has been propelled to the forefront at many universities. Materials science encompasses various classes of materials, including electronic materials, functional ceramics, magnesium, material and processes for flat-panel displays, eco/environmental materials, sustainable energy materials, transportation materials, electronic packaging materials, etc.

关键词： Barium compounds Crystal atomic structure Electronics packaging Flat panel displays Magnesium Materials handling Materials properties Materials science Nanotubes Nanowires Phenolic resins phenols Silica Silica gel Silicon nitride Single crystals

On the Absolute Configuration of Schweinfurthinol

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Chinese Journal of Chemistry 2011年第12期29卷 2759-2762页

作者：崔杉伍贻康高坡 School of Chemistry and Materials Heilongjiang University Harbin Heilongjiang 150080 China State Key Laboratory of Bioorganic and Natural Products Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

Through the chiral-pool based synthesis of both enantiomers of schweinfurthinol, it has been proven that this natural product isolated from the seeds of Canarium schweinfurthii possesses an （S） configuration. The op... 详细信息

关键词： natural products chiral building blocks diols phenols carbanions

Melamine-formaldehyde resin supported H^+:A mild and inexpensive reagent for synthesis of coumarins under mild conditions

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Chinese Chemical Letters 2011年第11期22卷 1313-1316页

作者： Ramin Rezaei Leila Dorosty Maryam Rajabzadeh Reza Khalifeh Department of Chemistry Firoozabad Branch Islamic Azad UniversityP.O.Box 74715-117 FiroozabadIran Department of Chemistry Faculty of Basic Sciences ShirazUniversity of TechnologyShiraz71555-313Iran

Melamine-formaldehyde resin supported H~＋ is used as an efficient catalyst in the Pechmann condensation reaction of phenols withβ-ketoesters,in solvent-free media leading to the formation of coumarin derivatives usi... 详细信息

关键词： Coumarins phenols β-Ketoesters Melamine-formaldehyde resin Pechmann condensation Solvent-free

A New Amperometric Detector Cell for Capillary Zone Electrophoresis

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Chinese Chemical Letters 1998年第8期9卷 757-760页

作者： Xiao Yun YANG JinYuan MO TianYao XIE Bing Yi YANG Wen Feng LIU Pei Xiang CAI(Department of Chemistry, Zhongshan University, Guang Zhou 510275) Zhongshan Univ Dept Chem Canton 510275 Peoples R China

A simple and convenient electrochemical detection cell for capillary electrophoresis is described in this paper. In this cell, alignment of the capillary working electrode with the separation capillary outlet can be achieved precisely and easily without the aid of the micropositioner or microscope. With end-column detection, the applicability of the cell as an amperometric detector for capillary electrophoresis is demonstrated by running several phenolic compounds in 50 mu m i.d, capillary with a capillary Pt electrode as the working electrode. Results obtained are satisfactory.

关键词： capillary electrophoresis electrochemical detection Pt electrode phenols

Silica-bonded S-sulfonic acid a recyclable catalyst for the synthesis of coumarins

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Chinese Chemical Letters 2009年第12期20卷 1444-1448页

作者： Khodabakhsh Niknam Dariush Saberi Mojtaba Baghernejad Department of Chemistry Faculty of SciencesPersian Gulf University

Silica-bonded S-sulfonic acid （SBSSA） was prepared by the reaction of 3-mercaptopropylsilica （MPS） and chlorosulfonic acid in chloroform. This solid acid has been employed as a recyclable catalyst for the synthesi... 详细信息

关键词： Silica-bonded S-sulfonic acid Coumarins Solvent-free phenols -Keto-esters

Polyvinylpolypyrrolidone-bromine complex:Mild and efficient polymeric reagent for bromination of activated aromatic compounds

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Chinese Chemical Letters 2011年第1期22卷 13-17页

作者： Masoud Mokhtary Moslem M.Lakouraj Department of Chemistry Islamic Azad University-Ayatollah Amoli BranchP.O.Box 678AmolIran Department of Organic-Polymer Chemistry Mazandaran UniversityBabolsar 47416Iran

Mild and efficient method for bromination of electron-rich aromatic compounds is described using polyvinylpolypyrrolidone- bromine complex （PVPP-Br2）. The reaction proceeded smoothly with phenols and N.N-alkylated a... 详细信息

关键词： Polyvinylpolypyrrolidone phenols Bromination Reagent

Oxalic acid catalyzed solvent-free one pot synthesis of coumarins

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Chinese Chemical Letters 2007年第11期18卷 1309-1312页

作者： Nagnnath D.Kokare Jaiprkash N.Sangshetti Devanand B.Shinde Department of Chemical Technology Dr.Babasaheb Ambedkar Marathwada University Aurangabad 431004 (MS)India Wockhardt Research CentreNew Drug DiscoveryAurangabad 431210 (MS)India Department of Chemical Technology Dr.Babasaheb Ambedkar Marathwada University Aurangabad 431004 (MS)India

Oxalic acid was found to be an efficient catalyst for Pechmann condensation, which includes the reaction between phenols and β-keto esters leading to formation of coumarin derivatives. The advantages of present methods are the use of cheap and easy available catalyst, solvent-free reaction conditions, better yields and shorter reaction time.

关键词： Coumarins Oxalic acid Solvent-free reaction Pechmann condensation β-Keto esters phenols

A Novel Synthesis of Some Hexasubstituted Cyclotriphosphazenes

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Chinese Chemical Letters 2001年第4期12卷 301-304页

作者： Cheng Feng YE Ze Fu ZHANG Wei Min LIU State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000

Some known and new hexaaryloxyphosphazene: N3P3(O-C6H4-R)(1)/(R=***3. C(CH3)(3). CHO.F.C6H5COOR. etc) were readily obtained with ca. 70% isolated yield in refluxing acetonitriie in the presence of anhydrous potassium ... 详细信息

关键词： Phosphazene cyclotriphosphazene phenols potassium phosphate