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中国植物病理学会2017年学术年会

作者： Tian Xin Shen Pan Zhang Song Zhou Changyong Cao Mengji National Citrus Engineering Research Center Citrus Research InstituteSouthwest University

A novel strain of Lettuce chlorosis virus（LCV） was identified from periwinkle（PW）with foliar interveinal chlorosis and plant dwarf in *** nucleotide（nt） sequences of genomic RNA1 and RNA2 of the virus are 8 602 nt and 8 456 nt,*** genomic organization of LCVPW resembles that of a California isolate（LCV-CA）,but its RNA2 only encodes 8 open reading *** LCV-PWRNA2 is shorter than that of LCV-CA,mainly due to two large *** analyses were performed using the amino acid sequences of the RdRp（ORFlb）,HSP70 h,coat protein（CP） and minor coat protein（CPm） of the two LCV isolates and representative members of the genus ***-joining trees show that the two LCV isolates are grouped together in a cluster containing Cucurbit chlorotic yellows virus（CCYV）,Bean yellow disorder virus（BnYDV）,Cucurbit yellow stunting disorder virus（CYSDV） and Tetterwort vein chlorosis virus（TVCV）.LCV-PWis different from LCV-CA by 84%（RNA1） and 89%（RNA2） at the genomic sequences and the bean isolate by 84.0%at the 421-nt HSP70 h sequence（KC602375,RNA2）,*** recently,a tomato isolate of LCV（KX158863） was identified from Shandong *** of a 446-nt partial la polyprotein sequence of the two Chinese isolates showed that they were 99.8%identical withone another,suggesting that they might bethe same variant.

关键词： Lettuce chlorosis virus Genome sequence Crinivirus periwinkle

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MicroRNA Regulation in Extreme Environments: Differential Expression of MicroRNAs in the Intertidal Snail Littorina littorea During Extended Periods of Freezing and Anoxia

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Genomics, Proteomics & Bioinformatics 2012年第5期10卷 302-309页

作者： Kyle K.Biggar Samantha F.Kornfeld Yulia Maistrovski Kenneth B.Storey Institute of Biochemistry&Department of Biology Carleton University

Several recent studies of vertebrate adaptation to environmental stress have suggested roles for microRNAs （miRNAs） in regulating glo- bal suppression of protein synthesis and/or restructuring protein expression patterns. The present study is the first to characterize stress- responsive alterations in the expression of miRNAs during natural freezing or anoxia exposures in an invertebrate species, the intertidal gastropod Littorina littorea. These snails are exposed to anoxia and freezing conditions as their environment constantly fluctuates on both a tidal and seasonal basis. The expression of selected miRNAs that are known to influence the cell cycle, cellular signaling pathways, carbohydrate metabolism and apoptosis was evaluated using RT-PCR. Compared to controls, significant changes in expression were observed for miR-la-1, miR-34a and miR-29b in hepatopancreas and for miR-la-1, miR-34a, miR-133a, miR-125b, miR-29b and miR-2a in foot muscle after freezing exposure at -6 ~C for 24 h （P 〈 0.05）. In addition, in response to anoxia stress for 24 h, significant changes in expression were also observed for miR-la-1, miR-210 and miR-29b in hepatopancreas and for miR-1 a-1, miR-34a, miR-133a, miR-29b and miR-2a in foot muscle （P 〈 0.05）. Moreover, protein expression of Dicer, an enzyme responsible for mature microRNA processing, was increased in foot muscle during freezing and anoxia and in hepatopancreas during freezing. Alterations in expression of these miRNAs in L. littorea tissues may contribute to organismal survival under freezing and anoxia.

关键词： Freeze tolerance Anaerobiosis Metabolic rate depression periwinkle Marine mollusk

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Hydrocarbon-Based Contaminants in Drinking Water Sources and Shellfish in the Soku Oil and Gas Fields of South-South Nigeria

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Open Journal of Yangtze Oil and Gas 2022年第4期7卷 213-230页

作者： Ifeanyichukwu Clinton Ezekwe Christian Livingstone Otiasah Morufu Olalekan Raimi Iyingiala Austin-Asomeji Department of Geography and Environmental Management University of Port Harcourt Amassoma Nigeria Department of Community Medicine Faculty of Clinical Sciences Niger Delta University Amassoma Nigeria Department of Community Medicine Faculty of Clinical Sciences College of Medical Sciences Rivers State University Port Harcourt Nigeria

Environmentally unfriendly Oil exploration activities have been ongoing in the Soku area of the Niger Delta of Nigeria since 1956. This study evaluated the concentration of hydrocarbons and heavy metals in Shellfish and drinking water sources in the study area. It revealed the absence (<0.001 mg/l) of most heavy metals (Ni, Ch, Cd, Pb mg/l) in the water column;a high concentration of the major ion composition of seawater (sulphates 5 - 1018;calcium 0.502 - 53.502;sodium 1.247 - 63.337;potassium 0.508 - 102.745;magnesium 0.354 - 42.574 mg/l);and high PAHs (<0.001 - 0.032 mg/l) levels occurring above WHO limits (0.007 mg/l) with some risk of exposure to cancer. Results from the analysis of shellfish showed that concentrations of chromium and zinc were below permissible limits while cadmium concentrations were slightly above permissible limits of the European Community. Nickel and lead were above permissible limits in the fish samples in all standards while PAHs occurred at the cancer risk levels of 10?6. A review of the public health situation in the Soku area with a view to understanding current trends, sources of perturbations and preferable solutions to the potential public health challenges raised in this study is hereby recommended. Also, this study recommends that relevant agencies and developmental partners should launch a national drive to create awareness among people/environmental/public health professionals’/health workers/administrators on this regional concern.

关键词： Niger Delta Oil and Gas Production Oil Pollution periwinkle Rural Livelihoods Water Quality

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Impact of Mixed Fillers on the Physico-mechanical Properties of Flexible Polyether Foam

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Organic Polymer Material Research 2021年第1期3卷 17-23页

作者： Onwuka,C.O Anekwe,O.J. Ogudo,M.C. Chris-Okafor,P.U. Department of Pure and Industrial Chemistry Faculty of Physical ScienceNnamdi Azikwe UniversityAwkaAnambra StateNigeria

The effect of proportional blend of periwinkle and African star apple seed shell as bio-fillers in flexible polyether foam was *** polyether foam samples incorporated with these bio-fillers at varying percentages;10%(S1),20%(S2),30%(S3),40%(S4)and 50%(S5)were produced respectively,while 0%(S0)which had no filler was used as control during the *** mechanical properties of the produced foam samples were determined via density,compression set,indentation hardness,tensile strength and elongation at break *** cream time,rise time and height of the foam as parameters for characterising the produced foam samples were determined *** test was also carried *** microstructure of the foam samples was analysed as well by using the scanning electron *** results of the experiment showed that the density of the foam samples progressively increased from 19.20(S0)-26.45(S5)as the quantity of the filler *** indentation hardness result also showed an increase on addition of the *** foam’s loading ability also increased on incorporation of the filler but S3 showed remarkable recovery after *** tensile strength and elongation at break of the foam decreased on addition of the *** morphological analysis ascertained the effect of the progressive introduction of the filler on the surface morphology of the *** flammability of the foam was found to decrease as the filler load *** these fillers are of organic origin,readily available,cheap and eco-friendly,they provide a means of making biodegradable foam,and reducing the flammability of ***,reducing environmental pollution whilst enhancing the mechanical property of foam.

关键词： Polyether foam periwinkle African star apple seed shell Mechanical properties Scanning electron microscopy

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