检索结果-南通市图书馆

peri-implantitis and periodontitis: Use of bacteriological test in dental practice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forensic Medicine and Anatomy Research 2013年第3期1卷 50-56页

作者： Francesco Carinci Rosa Maria Gaudio Department of Medical Sciences University of Ferrara Ferrara Italy Department of Morphology Surgery and Experimental Medicine University of Ferrara Ferrara Italy

peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reported, failures occur, and some implants are lost or removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. One of the major causes of the peri-implantitis is the bacterial colonization of implant surfaces but additional risk factors such as periodontitis, poor oral hygiene, tobacco consumption, prepost operative therapies and genetic susceptibility should be considered. In the present study a real-time PCR bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results demonstrated a significant higher prevalence of red complex species and increased amount of Porphyromonas gingivalis and Treponema denticolain periodontal pocket of periodontitis. Since a higher risk of peri-implantitis occurs in periodontally affected patients, detection and treatment of bacteria is a fundamental objective to ensure dental implant survival.

关键词： peri-implantitis periodontitis Microbia Bacterial Test Genetic Test

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Oral Science 2013年第4期5卷 212-216页

作者： Yan-Yun Wu Huan-Huan Cao Ning Kang Ping Gong Guo-Min Ou State Key Laboratory of Oral Diseases Department of ImplantologyWest China Hospital of StomatologySichuan University

Cellular fibronectin （cFn） is a type of bioactive non-collagen glycoprotein regarded as the main substance used to maintain periodontal attachment. The content of cFn in some specific sites can reflect the progress of periodontitis or peri-implantitis. This study aims to evaluate the expression of cFn messenger RNA （mRNA） in tissues of adult periodontitis and peri-implantitis by real-time fluorescent quantitative polymerase chain reaction （PCR） and to determine its clinical significance. A total of 30 patients were divided into three groups of 10： healthy, adult periodontitis and peri-implantitis. periodontal tissue biopsies （1 mmx I mmx I mm） from each patient were frozen in liquid nitrogen. Total RNA was extracted from these tissues, and the content, purity and integrity were detected. Specific primers were designed according to the sequence, and the mRNA expression levels of cellular fibronectin were detected by real-time PCR. The purity and integrity of the extracted total RNA were both high, and the specificity of amplified genes was very high with no other pollution. The mRNA expression of cFn in the adult periodontitis group （1.526＋0.441） was lower than that in the healthy group （3.253＋0.736）. However, the mRNA expression of cFn in the peri-implantitis group （3.965＋0.537） was significantly higher than that in the healthy group. The difference revealed that although both processes were destructive inflammatory reactions in the periodontium, the pathomechanisms were different and the variation started from the transcription level of the cFn gene.

关键词： adult periodontitis cellular fibronectin peri-implantitis real-time polymerase chain reaction

Investigation of Bacteria Species Most Involved in peri-implantitis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Open Journal of Stomatology 2023年第10期13卷 353-366页

作者： Hiroshi Murakami Osamu Tsuzukibashi Akira Fukatsu Yuji Takahashi Keisuke Idei Keisuke Usuda Mana Fuchigami Chiaki Komine Satoshi Uchibori Koji Umezawa Sachiyo Hayashi Takashi Asano Masanobu Wakami Taira Kobayashi Masahiko Fukumoto Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: Currently, bacteriological examinations of implant treatments target periodontopathic bacteria such as red complex bacteria, including Porphyromonas gingivalis, and detect them qualitatively or quantitatively. However, it seems that those examinations do not reflect the peri-implant tissue conditions precisely, because periodontopathic bacteria are also frequently detected from healthy peri-implant sites. The purpose of the present study was to investigate bacteria species most involved in peri-implantitis using a PCR method. Methods: Polymerase chain reaction (PCR) primers in this study were designed based on partial sequences of 16S rDNA of bacteria species involved in peri-implantitis that were described in numerous previous studies. peri-implant sulcus fluid (PISF) samples were collected from thirty periodontally healthy patients with implants (HI) and thirty patients with peri-implantitis (PI). Each detection frequency of bacteria species in PISFs of both groups was investigated using a PCR method, and was compared using Fisher’s exact test. Results: In PI group, detection frequencies of Corynebacterium durum, Fretibacterium fastidiosum and Slackia exigua were significantly higher than those of HI group (p P. gingivalis and Tannerella forsythia belonging to red complex were frequently detected in the PISF samples of HI group (p > 0.05). Conclusion: It was suggested that monitoring C. durum and F. fastidiosum levels in PISF samples was useful as a clinical indicator for the evaluation of peri-implant tissue conditions.

关键词： peri-implantitis PCR Method Bacteria Flora in peri-Implant Sulcus Red Complex

Dental Implant Patients Grouped by the Brinkman Index: Their Attitude toward Smoking, Nicotine Dependence, and Knowledge of peri-implantitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Regenerative Medicine 2016年第2期5卷 55-60页

作者： Ken Yukawa Noriko Tachikawa Shohei Kasugai Oral Implantology and Regenerative Dental Medicine Department of Masticatory Function Rehabilitation Tokyo Medical and Dental University Tokyo Japan

Purpose: This study investigated the attitude of patients, grouped by the Brinkman index, towards smoking by using the Kano Test for Social Nicotine Dependence (KTSND) and their knowledge of peri-implantitis. Methods: The participants were 3093 new patients who visited the Tokyo Medical and Dental University Hospital from January 2012 to December 2013 for an oral implant. The methodology included a questionnaire about sex, age, smoking status, daily average number of cigarettes, years of smoking, knowledge of peri-implantitis, and the KTSND. The patients were grouped according to their smoking status by calculating their Brinkman index (over 200 or not): current smokers with the possibility of nicotine dependence (BI(+)CS), current smokers with no possibility of nicotine dependence (BI(-)CS), ex-smokers (ES), and non-smokers (NS). The Brinkman index is obtained by multiplying one’s daily average number of cigarettes by the number of years they have been smoking. Results: Data were collected from 2182 respondents (response rate = 71%). The KTSND scores of BI(+)CS (16.89 ± 4.26) were significantly higher than the scores of ES (11.99 ± 4.52) and NS (11.53 ± 5.01). In current smokers, there were no significant differences between BI(+)CS and BI(-)CS. The patients replied “I don’t know” about peri-implantitis most often in all groups;however, there were no significant differences between the groups. Discussion: BI(+)CS were more dependent on nicotine in social situations than the other groups were. In Japan, a Brinkman index over 200 is required for a nicotine-dependence management fee to be instituted for health insurance treatment. This is a major concern for young smokers, who may be excluded from treatment because their years of smoking are substantially less. Results revealed that there were no significant differences between BI(+)CS and BI(-)CS. Therefore, it was suggested that the Brinkman index did not sufficiently group the participants.

关键词： Dental Implant peri-implantitis Smoking Smoking Cessation Kano Test for Social Nicotine Dependence (KTSND)

Comparative Cytological and Histopathological Study of peri-implantitis and periodontitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Stomatology 2023年第11期13卷 385-395页

作者： Masaaki Suemitsu Yoshikazu Nakayama Mao Yamamoto Mitsuko Nakayama Tadahiko Utsunomiya Kayo Kuyama Department of Pathology School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Pathology Nihon University Graduate School Dentistry at Matsudo Chiba Japan

peri-implant diseases, such as peri-implant mucositis and peri-implantitis, pose significant challenges to the long-term prognosis of dental implants. This study aimed to comprehensively compare peri-implantitis with periodontitis from cytological and histopathological perspectives, shedding light on the morphological characteristics associated with peri-implantitis. Thirteen patients, including six with peri-implantitis and seven with periodontitis, were included in the study. Cytological examination of affected gingival mucosa revealed distinct differences between the two conditions. peri-implantitis exhibited an inflammatory background predominantly composed of neutrophils with lobulated nuclei, accompanied by stratified squamous epithelial cells showing signs of keratinization. In contrast, periodontitis showed a similar neutrophilic inflammatory background but with non-keratinized epithelial cells. Histopathological examination further confirmed these differences, with peri-implantitis showing keratinized epithelium in the inner epithelial layer. This histological finding aligns with the notion that peri-implantitis has a distinct mucosal profile compared to periodontitis. Additionally, cytological analysis revealed that peri-implantitis had a lower occurrence rate of Light green-positive cells, indicating a tendency toward keratinization. This finding suggests that the presence of keratinized mucosa might be associated with peri-implant health, although further research is needed to clarify this relationship. Overall, this study demonstrates the potential of cytological examination and Papanicolaou staining for assessing mucosal inflammatory conditions and distinguishing between keratinized and non-keratinized cells. These findings underscore the utility of oral mucosal smears as a valuable tool for diagnosing peri-implantitis and enhancing our understanding of its pathogenesis.

关键词： peri-Implant Disease peri-implantitis Oral Cytology

Health Indicator Bacteria That Is Useful for Risk Assessment of peri-implantitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Stomatology 2021年第9期11卷 360-372页

作者： Hidenori Suzuki Osamu Tsuzukibashi Akira Fukatsu Department of Laboratory Medicine for Dentistry Nihon University Graduate School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine and Dentistry for the Compromised Patient Nihon University School of Dentistry Matsudo Chiba Japan

We established a novel identification method for oral Rothia species using one-step multiplex PCR analysis to investigate whether the monitoring of oral Rothia species levels is useful for peri-implantitis risk assessment, and to examine the oxygen concentration that these organisms need for growth in vitro. The mean number and proportion of Rothia aeria in peri-implant sulcus fluid (PISF) samples was significantly higher in the healthy implant group than in the peri-implantitis group (P R. aeria under aerobic conditions vigorously grew compared with those under anaerobic conditions, and this organism grew only at the upper layer where high oxygen concentrations existed in a semi-liquid nutrient medium. Therefore, the monitoring of R. aeria levels may be suitable as an indicator of healthy peri-implant tissue conditions for the prevention of peri-implantitis.

关键词： peri-implantitis Multiplex PCR Rothia aeria Oral Cavity

Role of Toll-like receptor 2 in inflammation and alveolar bone loss in experimental peri-implantitis ***

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of Toll-like receptor 2 in inflammation and alveolar bo...

Biofouling on titanium implants:a novel formulation of poloxamer and peroxide for in situ removal of pellicle and multi-species oral biofilm

中华口腔医学会全科口腔医学专业委员会第八次学术会议

作者：杨建军于新波 Han Xiaozhe 青岛大学附属医院

Objective and background:peri-implantitis and periodontitis are different entities in immune characteristics even though they share similar features in clinical and radiologic ***-like receptor 2(TLR2),one of the key pathogen recognition receptors in the innate immune system,plays an important role in the progress of ***,the role of TLR2 in peri-implantitis remains *** objective of this study was to investigate the role of TLR2 in inflammation and alveolar bone loss in a murine model of ligature-induced peri-implantitis and to compare it to ligature-induced ***:Smooth-surface titanium implants were placed in the alveolar bone of the left maxillary molars of Wild Type(WT) and TLR2 knockout(KO) mice 6 weeks after tooth *** ligatures were applied to the left implant fixtures and the right maxillary second molars to induce peri-implantitis and periodontitis 4 weeks after implant *** loss and inflammation around the implants and maxillary second molars were analyzed by micro-computed tomography(micro-CT) and histology and TRAP staining respectively 2 weeks after *** mRNA expressions of pro-inflammatory cytokines(IL-1β,TNF-α),anti-inflammatory cytokine(IL-10)and osteoclastogenesis-related cytokines(RANKL,OPG) were evaluated using real-time quantitative PCR(RT-qPCR).Results:Success Rate(SR) of Implant osseointegration was significantly greater in TLR2 KO mice(85.71%) when compared to WT mice(53.66%)(p=0.0125).Micro-CT revealed significantly decreased bone loss in TLR2 KO mice as compared to WT mice(p= 0.0094) in *** mRNA expressions of IL-1 b(p= 0.0055),TNF-α(p= 0.01) and IL-10(p= 0.0019) was significantly elevated in the peri-implantitis tissues of WT mice,but not in TLR2 KO mice,as compared to ***,the gingival mRNA ratios of RANKL/OPG in peri-implant tissues were significantly up-regulated in both WT(p=0.0488) and TLR2 KO mice(p= 0.0314).Ligature-ind

关键词： TLR2 bone resorption peri-implantitis periodontitis inflammatory cytokine

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2024年第6期3卷 60-72页

作者： Badra Hussain Roger Simm Jaime Bueno Savvas Giannettou Ali-Oddin Naemi Ståle Petter Lyngstadaas Håvard Jostein Haugen Department of Biomaterials Institute of Clinical DentistryUniversity of OsloOsloNorway Institute of Oral Biology University of OsloOsloNorway Section of the Postgraduate program in periodontology Faculty of DentistryComplutense UniversityMadrid(UCM)MadridSpain

Eradicating biofouling from implant surfaces is essential in treating peri-implant infections,as it directly addresses the microbial source for infection and inflammation around dental *** controlled laboratory study examines the effectiveness of the four commercially available debridement solutions‘(EDTA(Prefgel^(■)),NaOCl(perisolv^(■)),H_(2)O_(2)(Sigma-Aldrich)and Chlorhexidine(GUM^(■)Paroex^(■)))'in removing the acquired pellicle,preventing pellicle re-formation and removing of a multi-species oral biofilm growing on a titanium implant surface,and compare the results with the effect of a novel formulation of a peroxide-activated‘Poloxamer gel(Nubone^(■)Clean)'.Evaluation of pellicle removal and re-formation was conducted using scanning electron microscope(SEM),energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy to assess the surface morphology,elemental composition and chemical surface *** was assessed through contact angle *** multi-species biofilm model included Streptococcus oralis,Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans,reflecting the natural oral microbiome's *** biomass was quantified using safranin staining,biofilm viability was evaluated using confocal laser scanning microscopy,and SEM was used for morphological analyses of the *** indicated that while no single agent completely eradicated the biofilm,the‘Poloxamer gel'activated with‘H_(2)O_(2)'exhibited promising *** minimized re-contamination of the pellicle by significantly lowering the contact angle,indicating enhanced *** combination also showed a notable reduction in carbon contaminants,suggesting the effective removal of organic residues from the titanium surface,in addition to effectively reducing viable bacterial *** conclusion,the‘Poloxamer gel+H_(2)O_(2)'combination emerged as a promising chemical decontamination strategy for peri-implant diseases

关键词： chemical decontamination cleaning of implant surfaces complex oral biofilms pellicle formation peri-implantitis

Effects of combined chemical design(Cu addition) and topographical modification(SLA) of Ti-Cu/SLA for promoting osteogenic, angiogenic and antibacterial activities

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2020年第12期47卷 202-215页

作者： Rui Liu Yulong Tang Hui Liu Lilan Zeng Zheng Ma Jun Li Ying Zhao Ling Ren Ke Yang Institute of Metal Research Chinese Academy of SciencesShenyang 110016China Institute of Molecular Medicine State Key Laboratory of Oncogenes and Related GenesShanghai Institute of CancerRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200011China Department of Stomatology General Hospital of Shenyang Military CommandShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230026China Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen 518055China

Cu has been proved to possess various beneficial biological activities, while sandblasting and acid etching(SLA) is widely used to modify the commercial dental implant in order to improve osseointegration. Based on the above, a novel antimicrobial dental implant material, Ti-Cu alloy, was treated with SLA, to combine chemical design(Cu addition) and topographical modification(SLA). In this work, the effects of SLA treated Ti-Cu alloys(Ti-Cu/SLA) on osteogenesis, angiogenesis and antibacterial properties were evaluated from both in vitro and in vivo tests, and Ti/SLA and Ti-Cu(without SLA) were served as control groups. Benefiting by the combined effects of chemical design(Cu addition) and micro-submicron hybrid structures(SLA),Ti-Cu/SLA had significantly improved inhibitory effects on oral anaerobic bacteria(P. gingivalis and ***) and could induce upregulation of osteogenic-related and angiogenic-related genes expression in vitro. More importantly, in vivo studies also demonstrated that Ti-Cu/SLA implants had wonderful biological performance. In the osseointegration model, Ti-Cu/SLA implant promoted osseointegration via increasing peri-implant bone formation and presenting good bone-binding, compared to Ti/SLA and Ti-Cu implants. Additionally, in the peri-implantitis model, Ti-Cu/SLA effectively resisted the bone resorption resulted from bacterial infection and meanwhile promoted osseointegration. All these results suggest that the novel multiple functional Ti-Cu/SLA implant with rapid osseointegration and bone resorption inhibition abilities has the potential application in the future dental implantation.

关键词： Ti-Cu SLA peri-implantitis Osseointegration