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Ganoderma Lucidum polysaccharide peptides protect kidney from renal ischemia reperfusion injury via counteracting oxidative stress

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中国药理学与毒理学杂志 2015年第S1期29卷 72-73页

作者： Dan-danZHONG Hong-kaiWANG MingLIU Ying-liJIA MingHUANG HongZHOU Shu-qianLIN Zhi-binLIN Bao-xueYANG State Key Laboratory of Natural and Biomimetic Drugs Department of Pharmacology School of Basic Medical Sciences Peking University Beijing China Fuzhou Institute of Green Valley Bio-Pharm Technology Fuzhou China Juncao Technology Research Institute Fujian Agriculture and Forestry University Fuzhou China

OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant *** oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative *** Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against *** GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the *** vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative *** mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological *** Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP *** abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP *** apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell *** Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury.

关键词： Ganoderma lucidum polysaccharide peptides acute ki

peptides and Peptidomics: A Tool with Potential in Control of Plant Viral Diseases

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Advances in Microbiology 2014年第9期4卷 539-548页

作者： José Silvestre Mendoza-Figueroa Manuel Soriano-García Laura Beatriz Valle-Castillo Jesús Méndez-Lozano Instituto de Química Universidad Nacional Autónoma de México Ciudad Universitaria Coyoacán Mexico City Mexico Instituto Politécnico Nacional CIIDIR Unidad Sinaloa Guasave Mexico

Plant viruses are the most infectious agents in commercially important crops worldwide. Plant viral diseases are important because both decreased yielding and quality of fruits, flowers or vegetables lead to million-dollar losses in production. At present there are no reports which suggest a direct control of plant virus. A new strategy for plant virus control has been raised since 13 years ago—the use of peptides. peptides could offer a direct interaction by affinity selection against viral proteins involved in infection cycle, like capsid or movement protein (e.g.) and affect viral replication. Peptidomics, as a new tool to study peptides, led us screening and selecting the best peptide with antiviral activity, and re-designing it to enhance the biological effect as well as the potential of bioactivity of those peptides secreted by microbes present in soil. In this paper we review current aspects in the use of peptides and peptidomics as a strategy to study new methods that lead a direct control against plant viral diseases.

关键词： Peptidomics Plant Virus peptides Peptaiboles Mass Spectrometry Aptamers

peptides and Exorphins in the Autism Spectrum

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Open Journal of Psychiatry 2014年第3期4卷 275-287页

作者： Dag Tveiten Adrian Finvold Marthe Andersson Karl LReichelt Lab 1 Harbitzalle 3SkoyenOsloNorway Medical Faculty Oslo UniversityOsloNorway

Problem: Some researchers have not found the opioids in urine of autistic children. We have therefore looked at this problem again. Method: Mass spectrometry and fragmentation mass spectrometry (MS/MS) have been carried out on peaks from the HPLC that show co-chromatography with synthetic standards and peaks that are shared by different autistic children. Results: In quickly frozen urine we find the presence of exorphins, and can also demonstrate a rather fast break down at room temperature of these peptides in urine. Conclusion: Exorphins are present in urine in autistic children, but must be protected against break down and aggregation by fast freezing or acetic acid and adjusting declustering potential and collision potential during mass-spectroscopy. Specific antibody increases and the effect of removing precursor proteins from the diet reinforce this view.

关键词： Autism Behavior Diet Exorphins Mass-Spectrometry peptides

peptides as Asymmetric Catalysts

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Peptides as Asymmetric Catalysts

Evaluating the physicochemical effects of conjugating peptides into thermogelling hydrogels for regenerative biomaterials applications

中国化学会第27届学术年会

作者： Helma Wennemers University of Basel Department of Chemistry

＜正＞In organic synthesis, enzymes and man-made catalysts are commonly used. We are intrigued by the question whether short-chain peptides may be useful alternatives to enzymes and catalysts of low

关键词： organocatalysis peptides conjugate addition reactions.

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Regenerative Biomaterials 2021年第6期8卷 260-272页

作者： Hannah A.Pearce Emily Y.Jiang Joseph W.R.Swain Adam M.Navara Jason L.Guo Yu Seon Kim Andrew Woehr Jeffrey D.Hartgerink Antonios G.Mikos Department of Bioengineering Rice University6500 Main StreetHoustonTX 77030USA Depatment of Chemistry Rice University6500 Main StreetHoustonTX 77030USA

Thermogelling hydrogels,such as poly(N-isopropylacrylamide)[P(NiPAAm)],provide tunable constructs leveraged in many regenerative biomaterial ***,our lab developed the crosslinker poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol),which crosslinks P(NiPAAm-co-glycidyl methacrylate)via thiol-epoxy reaction and can be functionalized with azide-terminated peptides via alkyne-azide click *** study’s aim was to evaluate the impact of peptides on the physicochemical properties of the *** physicochemical properties of the hydrogels including the lower critical solution temperature,crosslinking times,swelling,degradation,peptide release and cytocompatibility were *** gels bearing peptides increased equilibrium swelling indicating hydrophilicity of the hydrogel *** sol fractions were found for all groups,indicating that inclusion of peptides does not impact ***,the inclusion of a matrix metalloproteinase-sensitive peptide allowed elucidation of whether release of peptides from the network was driven by hydrolysis or enzymatic *** hydrophilicity of the network determined by the swelling behavior was demonstrated to be the most important factor in dictating hydrogel behavior over *** study demonstrates the importance of characterizing the impact of additives on the physicochemical properties of *** characteristics are key in determining design considerations for future in vitro and in vivo studies for tissue regeneration.

关键词： thermogelling click chemistry hydrogels peptides poly(N-isopropylacrylamide)

Expression of Fusion Lytic peptides Promotes Fungal Disease Resistance in Transgenic Plants

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American Journal of Plant Sciences 2020年第2期11卷 148-161页

作者： George S. Biliarski Bakhyt Yertaeva Amitava Mitra University of Nebraska Department of Plant Pathology Lincoln NE USA Institute of Plant Biology and Biotechnology Almaty Kazakhstan

Many organisms produce small proteins which exhibit antimicrobial activities. In recent decades, the biological role of antimicrobial peptides (AMP) has been recognized as the main factor in the defense mechanisms against a broad range of pathogenic microbes. The increased worldwide incidence of microbial resistance to antibiotics makes AMPs promising alternative for the control of microbial disease. Exploring the potential of AMPs in transgenic crops could lead to the development of new and improved cultivars which are resistant to various economically important diseases. In the present study, two fusion lytic peptide gene constructs coding for antimicrobial peptides were expressed in Nicotiana benthamiana tobacco plants and tested against three fungal pathogens, Sclerotinia sclerotiorum, Rhizoctonia solani, and Pythium sp. Detached-leaf bioassay was employed for the transgenic plants carrying the fusion lytic peptide constructs (ORF13 and RSA1), transgenic vector only control plants (1234), and wild-type control plants (WT) against the three fungal pathogens. Symptom area of each leaf was measured with high accuracy and data were recorded and processed by statistical analyses. The results showed that transgenic plant lines ORF13 and RSL1 have substantial resistance to Sclerotinia sclerotiorum infection, producing significantly smaller lesion areas compared to vector only plant line 1234 and wild type plants. These transgenic lines also provided resistance against Rhizoctonia solani, however, these lines were not effective against the other fungal pathogen Pythium sp.

关键词： Antimicrobial peptides Antibiotic Resistance Fungal Diseases Plant Disease Control Transgenic Plants

A New Generation of Drugs: Synthetic peptides Based on Natural Regulatory peptides

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Neuroscience & Medicine 2013年第4期4卷 223-252页

作者： Timur Kolomin Maria Shadrina Petr Slominsky Svetlana Limborska Nikolay Myasoedov Department of Chemistry of Physiologically Active Compounds Institute of Molecular Genetics Russian Academy of Sciences Moscow Russia Department of Molecular Basis of Human Genetics Institute of Molecular Genetics Russian Academy of Sciences Moscow Russia

Natural regulatory peptides are biologically active compounds that are produced by various cells and provide a link among the main regulatory systems of the body. The field of research into the biologic activity of endogenous regulatory peptides is extremely vast. These peptides affect the cardiovascular, immune, reproductive, endocrine, digestive, and other systems, alter energy metabolism, and are especially effective in the regulation of the central nervous system. Despite of the wide range of preventive and therapeutic effects of natural regulatory peptides and proteins, their application in clinical practice is difficult. This is primarily because of their extreme instability, as they are rapidly degraded by proteases of the gastrointestinal tract, blood, cerebrospinal fluid, and other biologic media. Compounds with higher stability (i.e., a considerably longer half-life compared with that of natural molecules) and the ability to provide a directional effect on the various body systems were obtained from modifications of endogenous regulatory peptides. Synthetic analogs of regulatory peptides, as a rule, contain only natural amino acids in their composition, and their biodegradation does not lead to the formation of toxic products;thus, they have fewer side effects. This review focuses on the consideration of two synthetic regulatory peptides, Semax and Selank, which were the bases for the creation of new drugs that are used effectively in the treatment of various diseases of the nervous system. The synthetic analog of an adrenocorticotropic hormone 4-10 fragment (ACTH4-10) Semax is a powerful neuroprotective agent that is particularly effective as a therapy for stroke. Selank was synthesized on the basis of the natural immunomodulator tuftsin. Selank is a powerful anxiolytic that is used as a therapy for generalized anxiety disorder and neurasthenia without sedative and muscle-relaxant effects. This review presents the results of research aimed at studying the

关键词： Semax Selank Regulatory peptides ACTH Tuftsin Neuroprotection Nootropes Anxiolytics

Novel Anti-Cancer peptides Comprising Three Amino Acids

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Journal of Cancer Therapy 2012年第4期3卷 230-236页

作者： Michael Agrez Madhu Garg Stephen Ackland Department of Medical Oncology Calvary Mater Hospital Waratah New South Wales Australia Division of Surgery John Hunter Hospital Newcastle New South Wales Australia University of Newcastle Faculty of Health Callaghan New South Wales Australia

Background: The NPxY motif common to all β integrin cytoplasmic domains forms part of a canonical recognition sequence for phosphotyrosine-binding domains which are protein modules present in a wide variety of signaling and cytoskeletal proteins. We have recently reported that a non-naturally occurring peptide, RSKAKNPLYR, derived from the β6 integrin cytoplasmic domain inhibits cancer cell growth in vitro and proposed that this may be due, at least in part, to the inhibition of c-Src activity [1]. In the present study we examined the role of the NPLY motif within RSKAKNPLYR in terms of its requirement for inhibition of cancer cell growth. Materials and Methods: The effects of peptide modifications to RSKAKNPLYR on in vitro proliferation of human cancer cell lines (colorectal HT29, prostate DU145, breast MCF-7 and ovarian A2780) were evaluated using the MTT cell growth assay. Passage of peptide across the plasma membrane was assessed by means of confocal microscopy using FITC-labelled peptide. The effect of peptide on kinase activity was assessed in cell-free in vitro kinase assays. Results: The NPLY motif within RSKAKNPLYR was found to be essential for the growth inhibitory effect of this peptide. However, modified forms of this peptide in which all amino acids except the charged residues arginine and lysine were replaced by single non-polar amino acids such as alanine or valine were equally effective at inhibiting cancer cell proliferation. Moreover, these peptides inhibited not only c-Src activity as seen for RSKAKNPLYR but also the activity of members of the PKB/Akt kinase family. Conclusion: Novel decapeptides comprising only three amino acids have anti-cancer effects without the requirement for an integrin-based NPLY motif. These peptides inhibit the activity of not only c-Src but also members of the Akt family of kinases and may be useful as potential anti-cancer agents when used either alone or in combination with compounds previously reported to inhibit c-S

关键词： peptides Cancer Cell Lines MTT Assay Kinase Activity c-Src Akt

Effects of Pyridoxine on Selected Appetite Regulating peptides mRNA Expression in Hypothalamic PVN/ARC Nuclei and Gastrointestinal Tract Tissues

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Advances in Bioscience and Biotechnology 2017年第9期8卷 273-282页

作者： Lei Liu Haoqi Wang Haitao Sun Chunyan Fu Hongli Liu Yuqing Sun Xianghua Xu Weiji Chen Wenqiang Wu Fuchang Li Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Department of Animal Science and Technology Shandong Agricultural University Taian China Animal Husbandry and Veterinary Institute Shandong Academy of Agricultural Sciences Jinan China Poultry Institute Shandong Academy of Agricultural Sciences Jinan China

An experiment was conducted to investigate the effect of dietary pyridoxine on the gene expression of appetite-regulating peptides in the hypothalamus and gastrointestinal tract of rabbits. Thirty-two rabbits were randomly divided into 2 treatments for 8 weeks (16 replicates/group and 1 rabbit/replicate). The treatments were fed a basal diet (control, measured pyridoxine content is 4.51 mg/kg) and the basal diet with a pyridoxine supplementation at 10 mg/kg (pyridoxine, measured pyridoxine content is 14.64 mg/kg). The results showed that dietary pyridoxine did not significantly alter the mRNA levels of neuropeptide Y, agouti related peptide, pro-opiomelanocortin and cocaine, amphetamine regulated transcript, peptide YY and cholecystokinin in arcuate nucleus, peptide YY in jejunum and ileum, and cholecystokinin in duodenum, jejunum and ileum (P > 0.05). Compared with the control, the mRNA levels of corticotropin-releasing hormone and melanocortin 4 receptor in paraventricular nuclei and peptide YY in duodenum were significantly decreased after pyridoxine treatment (P 0.05). In conclusion, the appetite genes of melanocortin 4 receptor and corticotropin-releasing hormone in paraventricular nuclei and peptide YY in duodenum are involved in the pyridoxine-caused hyperphagia.

关键词： Pyridoxine Brain-Gut peptides Appetite Control