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Computational identification and characterization of microRNAs and their targets in penaeus monodon

Journal of Oceanology and Limnology 2018年第3期36卷 853-869页

作者： PU Longjun WANG Jing WANG Yu ZUO Jianwei GUO Huarong Ministry of Education Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China

Study on shrimp miRNAs was limited and just 7 mature miRNA sequences of Marsupenaeus japonicus are deposited in mir Base database. In this study, miRNAs and their target gene candidates were computationally identified from shrimp Penaeu s monodon and then experimentally validated. Using 39 908 expressed sequence tags(ESTs) and 21 124 genome survey sequences(GSSs) of P. monodon(pmo) as reference dataset, a comprehensive approach based on inter-species homolog search was employed to investigate the candidate miRNAs(i.e. pmo-miRNA). A total of eight miRNAs belonging to 7 families were computationally identified and five out of them were subsequently validated by PCR and sequencing. Of these, pmo-miR-4961a, pmo-miR-4961b, pmo-miR-4979 and pmo-miR-3819 were first identified from shrimps. Both the mature pmo-miRNAs and the corresponding precursors were conserved among different species. Based on perfect or near-perfect match to the target region, the target gene candidates of pmomiRNAs were predicted from 10 331 mRNA sequences of P. monodon. A total of 20 genes were predicted as the targets of pmo-miR-4961a, pmo-miR-4961b, pmo-miR-4979 and pmo-miR-6492. Experimental validation by dual luciferase reporter assay confi rmed the targeting between 3 pmo-miRNAs and one or two of their target genes, especially the pmo-miR-4979 which could significantly down-regulate the expression of target gene(JR226772). This study updates the miRNAs and their targets in P. monodon and lays a solid foundation for future RNAi study.

关键词： microRNA shrimp penaeus monodon target genes

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Preliminary studies on form, structure and function of androgenic gland in penaeus chinensis

引用

Chinese Science Bulletin 1997年第6期42卷 499-503页

作者： Li, FH Xiang, JH Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

SCIENTISTS have paid much attention to sex control of animals. Sex control of shrimp is a noticeable topic in the field of high technology in aquaculture. Increasing the proportion of female shrimps in a rearing population is undoubtedly a very effective path to obtain higher aquacultural production of penaeus chinensis. But up to date, the mechanism of sex differentiation and determination in shrimp is not clear. In recent years, we have done some work on

关键词： penaeus chinensis androgenic gland function.

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Isozyme　changes　during　larval　development　of　penaeus　chinensis

引用

Acta Oceanologica Sinica 1997年第2期16卷 215-224页

作者： Zhang Z. Ma Y. Liao C. Wang H. College of Marine Life Sciences Ocean University of Qingdao Qingdao 266003 China

ＩｓｏｚｙｍｅｃｈａｎｇｅｓｄｕｒｉｎｇｅａｒｌｙｄｅｖｅｌｏｎｔｏｆＰｅｎａｅｕｓｃｈｉｎｅｎｓｉｓａｒｅｄｅｓｃｒｉｂｅｄｆｏｒｓｉｘｅｎｚｙｍｅｓ（ＥＳＴ，ＡＭＹＭＤＨ，ＧＤＨ，ＧｄａｎｄＬＤＨ）ｂｙｍｅａｎｓ... 详细信息

ＩｓｏｚｙｍｅｃｈａｎｇｅｓｄｕｒｉｎｇｅａｒｌｙｄｅｖｅｌｏｎｔｏｆＰｅｎａｅｕｓｃｈｉｎｅｎｓｉｓａｒｅｄｅｓｃｒｉｂｅｄｆｏｒｓｉｘｅｎｚｙｍｅｓ（ＥＳＴ，ＡＭＹＭＤＨ，ＧＤＨ，ＧｄａｎｄＬＤＨ）ｂｙｍｅａｎｓｏｆｐｏｌｙａｃｒｙｌａｍｉｄｅｇｅｌｖｅｒｔｉｃａｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ．ＴｈｅｒｅｓｕｌｔｓｓｈｏｗｔｈａｔｔｈｅｎｕｍｂｅｒｏｆｉｓｏｚｙｍｅｓｖａｒｉｅｓｃｏｎｓｉｄｅｒａｂｌｙａｎｄｔｈｅｅｎｚｙｍｅａｃｔｉｖｉｔｙａｌｓｏｒｉｓｅｓｇｒａｄｕａｌｌｙｗｉｔｈｄｅｖｅｌｏｐｍｅｎｔＴｈｅｂａｎｄｎｕｍｂｅｒｏｆＥＳＴｉｎｃｒｅａｓｅｓｆｒｏｍ１ｉｎｎａｕｐｌｉｕｓｓｔａｇｅｔｏ６ｉｎｐｏｓｔｌａｒｖａｌｓｔａｇｅＴｈｅｒｅａｒｅｔｈｒｅｅｔｙｐｅｓｏｆＡＭＹ，ａ－ＡＭＹ，Ｒ－ＡＭＹ，ａｎｄＱ－ＡＭＹ．ＴｈｅｐｈｅｎｏｔｙｐｅｏｆＬｄｈｃｈａｎｇｅｓｓｌｉｇｈｔｌｙ，ｓｈｏｗｉｇｎｔｈｅｓａｍｅｐａｔｔｅｒｎｅｓｃｅｐｔｎａｕｐｌｉｕｓｉｎａｌｌｔｈｅｓｔａｇｓ．Ｔｈｅｒｅｍａｉｎｉｎｇｔｈｒｅｅｅｎｚｙｍｅｓ（ＭＤＨ，ＧｄａｎｄＧＤＨ）ｈａｖｅｍｏｒｅｂａｎｄｓａｎｄｔｈｅｉ

关键词： larval penaeus changes chinensis development Isozyme

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STUDIES ON IMMUNODIAGNOSIS OF HEPATOPANCREATICPARVO-LIKE VIRUS DISEASES OF penaeus ORIENTALISII. RAPID EARLY DIAGNOSIS

引用

Chinese Journal of Oceanology and Limnology 1993年第2期11卷 245-248页

作者：孙修勤王文兴邵济钧张进兴卢颖薛清刚宋庆云 First Institute of Oceanography SOA Qingdao 266003 Qingdao Medical College Qingdao 266012

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory *** is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid *** SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.

关键词： penaeus orientalis hepatopancreatic viral disease immune serum (IS) SPA coagglutination

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Lactate dehydrogenase C in sperm of shrimp (penaeus orientalis)

引用

Chinese Science Bulletin 1995年第2期40卷 154-157页

作者：陈明周念辉李爱媛徐科孙海宝 Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai 200031 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China.

Lactate dehydrogenase (LDH, EC 1.***.1.27), widely existing in different tissues of various species of animals, catalyzes the reversible interconversion of lactate and pyruvate. In mammals and birds, it is a tetramer composed of A-subunit and B-subunit. It has been established that there are five LDH isozymes with different combinations of A- and B-subunit proportions. In skeletal muscle, A-subunit is the major component; whereas

关键词： penaeus orientalis sperm mascle lactate dehydrogenase C.

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ONTOGENIC STUDY OF ISOZYMES IN GIANT TIGER PRAWN,penaeus MONODON (CRUSTACEA:DECAPODA)

引用

Chinese Journal of Oceanology and Limnology 1996年第4期14卷 316-321页

作者：童金苟 E.Ballment J.A.H.Benzie Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 Australian Institute of Marine Science PMB 3 Townsville Qld 4810. Australia

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase.

关键词： isozymes ontogeny penaeus monodon

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ON THE ULIRASTRUCTURE AND CLASSIFICATION OF THE HEMOCYTES OF PENAEID SHRIMP, penaeus VANNAMEI (CRUSTACEA, DECAPODA)

引用

Chinese Journal of Oceanology and Limnology 1998年第4期16卷 333-338页

作者：刘恒王雷

The ultrastructure of hemocytes of penaeus vannamei was examined by transmissionelectron mircroscope. Three types of hemocytes were identified: hyaline cell, small-granule cell andlarge-granule cell. Hyaline cells are the smallest of the hemocytes, lack electron-dense granules, haveaverage size of 11. 11 μp (SE ± 0.50, n = 10) × 6.00 μp (SE ± 0.55, n = 10), comprise 25. 8%(SE ± 2.87, n = 4 ×10) of the hemocyt; small granule cells are the mos abundan type of hamocytes,cornprise 58. 1% (SE ± 3.4o, n = 4 ×10) of the hees, contaln sInall electron - dense ,haVe average sbe of 10.78 mp (SE ± 0.00, n = 10) × 8.63 mp (SE ± 0.44, n = 10); and largn-gran-ule cells comprise 16. 1% (SE ± 2. 55, n = 4 × 10) of the hemocytes, are filled with large elecbondense guules, have average size of 12.51 μp (SE ± 0.63, n = 10) × 8.99μm (SE ±0.71, n = 10).

关键词： penaeus vannamei hemocyte ultrastructure classification

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DETECTION OF WHITE SPOT SYNDROME VIRUS(WSSV) OF penaeus CHINENSIS BY IN SITU HYBRIDIZATION

引用

Chinese Journal of Oceanology and Limnology 2000年第3期18卷 241-246页

作者：战文斌王远红张志栋青岛海洋大学教育部海水养殖重点实验室山东青岛 2 660 0 3 Tokyo University of Fisheries 日本 Tokyo

White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles, EcoR I digested fragments of the WSSV genome were cloned; three of these fragments were used as non radioactive probes labeled with DIG 11 dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV infected tissues. The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.

关键词： WSSV penaeus chinensis in situ hybridization

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Pathogens and pathogenicity to penaeus orientalis Kishinouye

引用

Acta Oceanologica Sinica 1994年第2期13卷 297-304页

作者： $$$$

ＰａｔｈｏｇｅｎｓａｎｄｐａｔｈｏｇｅｎｉｃｉｔｙｔｏＰｅｎａｅｕｓｏｒｉｅｎｔａｌｉｓＫｉｓｈｉｎｏｕｙｅＩＮＴＲＯＤＵＣＴＩＯＮＢａｃｔｅｒｉａ，ｅｓｐｅｃｉａｌｌｙｖｉｂｒｉｏｓａｒｅｏｎｅｏｆｔｈｅｍａｊｏｒ... 详细信息

ＰａｔｈｏｇｅｎｓａｎｄｐａｔｈｏｇｅｎｉｃｉｔｙｔｏＰｅｎａｅｕｓｏｒｉｅｎｔａｌｉｓＫｉｓｈｉｎｏｕｙｅＩＮＴＲＯＤＵＣＴＩＯＮＢａｃｔｅｒｉａ，ｅｓｐｅｃｉａｌｌｙｖｉｂｒｉｏｓａｒｅｏｎｅｏｆｔｈｅｍａｊｏｒｐａｔｈｏｇｅｎｓｔｏｃｕｌｔｕｒ...

关键词： pathogenicity penaeus

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TRIPLOIDY INDUCTION WITH HEAT SHOCKS TO penaeus CHINENSIS AND THEIR EFFECTS ON GONAD DEVELOPMENT

引用

Chinese Journal of Oceanology and Limnology 1999年第1期17卷 57-61,49页

作者：李富花周令华相建海刘旭东朱谨钊

Heat shocks effectively produced triploids in penaeus chinensis . Fertilized eggs heat shocked (28-32℃) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%-75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male shrimp, there were no such differences.

关键词： triploidy induction penaeus chinensis heat shock

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