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Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without pediatric chronic rhinosinusitis： A Preliminary Report

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Chinese Medical Journal 2015年第21期128卷 2913-2918页

作者： Xiao-Peng Qu Zhen-Xiao Huang Yan Sun Ting Ye Shun-Jiu Cui Qian Huang Li-Jing Ma Qing-Wen Yang Hong Wang Er-Zhong Fan Ying Li Liang Zhang Bing Zhou Department of Otolaryngology-Head and Neck Surgery Beijing Tongren Hospital Capital Medical University Beijing 100730 China Department of Otolaryngology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Key Laboratory of Otolaryngology-Head and Neck Surgery Ministry of Education Beijing Institute of Otolaryngology Beijing 100005 China

Background： Adenoid hypertrophy （AH） is associated with pediatric chronic rhinosinusitis （pCRS）, but its role in the inflammatory process of pCRS is unclear. It is thought that innate immunity gene expression is disrupted in the epithelium of patients with chronic rhinosinusitis （CRS）, including antimicrobial peptides and pattern recognition receptors （PRRs）. The aim of this preliminary study was to detect the expression of innate immunity genes in epithelial cells of hypertrophic adenoids with and without pCRS to better understand their role in pCRS. Methods： Nine pCRS patients and nine simple AH patients undergoing adenoidectomy were recruited for the study. Adenoidal epithelium was isolated, and real-time quantitative polymerase chain reaction （RT-qPCR） was employed to measure relative expression levels of the following messenger RNAs in hypertrophic adenoid epithelial cells of pediatric patients with and without CRS： Human β-defensin （HBD） 2 and 3, surfactant protein （SP）-A and D, toll-like receptors 1-10, nucleotide-binding oligomerization domain （NOD）-like receptors NOD 1, NOD 2, and NACHT, LRR and PYD domains-containing protein 3, retinoic acid-induced gene 1, melanoma differentiation-associated gene 5, and nuclear factor-riB （NF-KB）. RT-qPCR data from two groups were analyzed by independent sample t-tests and Mann-Whitney U-tests. Results： The relative expression of SP-D in adenoidal epithelium ofpCRS group was significantly lower than that in AH group （pCRS 0.73 ± 0.10 vs. AH 1.21 ±0.15; P = 0.0173, t = 2.654）. The relative expression levels of all tested PRRs and NF-κB, as well as HBD-2, HBD-3, and SP-A, showed no statistically significant differences in isolated adenoidal epithelium between pCRS group and AH group. Conclusions： Down-regulated SP-D levels in adenoidal epithelium may contribute to the development of pCRS. PRRs, however, are unlikely to play a significant role in the inflammatory process ofpCRS.

关键词： Adenoids: Epithelial Cell Innate Immunity: Pattern Recognition Receptors pediatric chronic rhinosinusitis

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