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Enzymatic Characterization of Pectinex XXL,a pectinase Produced by Aspergillus niger,and Its Application in Fruit Juice Production

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现代食品科技 2024年第7期40卷 81-88页

作者： ZHU Furong LIANG Xiaomei WEI Zhifu LI Li HE Fengyuan CHENG Zhong College of Food and Quality Engineering Nanning UniversityNanning 530200China Guangxi Bingke Food Co.Ltd. Nanning 530100China

Pectinex XXL,a commercially prepared pectinase,was investigated for its potential application in the fruit juice *** acid was used as the substrate for determining the enzymatic properties of Pectinex XXL using the DNS *** to the results,the optimal pH for Pectinex XXL activity was 4.5,and the enzyme was stable in the pH range of 3.0~*** optimal pH and pH stability range are consistent with those of some tropical and subtropical *** optimal temperature for Pectinex XXL activity was 60℃,and the enzyme remained stable after one hour in a water bath set at 40℃.Additionally,the enzymatic activity was not inhibited in the presence of 1 mmol/L of Na^(+),Mg^(2+),Ba^(2+),Co^(2+),Zn^(2+),and Fe^(2+),whereas it was slightly inhibited in the presence of 2 mmol/L of K^(+)and Fe^(2+)and partially inhibited in the presence of 1 and 2 mmol/L of Ca^(2+)and Mn^(2+),demonstrating its good stability in acids and excellent thermal catalytic *** on the above experimental results,depectinization experiments were performed on plantain and cherry tomato juices using different amounts of Pectinex *** one hour reaction with 16 U/mL of the enzyme,the yields of the plantain and cherry tomato juices were substantially increased by 119.03%and 15.97%,respectively,while their light transmittance was remarkably enhanced by 37.65%and 12.35%,***,the enzyme reduced the viscosity of the plantain and cherry tomato juices by 88.29%and 29.50%,*** juice production experiments confirmed that this enzyme can significantly improve the yield and light transmittance of plantain juice,while effectively reducing its *** findings indicate the potential of Pectinex XXL in the industrial production of plantain juice.

关键词： plantain cherry tomato fruit juice Aspergillus niger pectinase

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pectinase Production by Aspergillus Niger P-6021 on Citrus Changshan-huyou Peel in Slurry-state Fermentation

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Chinese Journal of Chemical Engineering 2005年第4期13卷 510-515页

作者：钟卫鸿岑沛霖 Department of Biotechnology Zhejiang University of Technology Key Lab of Renewable Resources of Ministry of Chemical Industry Depaertment of Chemical & BiochemicalEngineering Zhejiang University

The peel of Citrus changshan-huyou, coupled with wheat bran, could be utilized by Aspergillus niger P-6021 in slurry-state fermentation to produce pectinase with suitable enzyme composition for application in apple juice processing. The production of pectinase is improved by additional nitrogen source substances and mineral supplements. The ratio of carbon source substances to nitrogen source substances in the medium also has significant effect on the pectinase production by A. niger P-6021 in slurry-state fermentation. In the optimized medium composition, the maximal enzyme activity could reach 42 U.L^- 1 （polymethylgalacturonase）, 6.7 U.L^- 1 （polymethygalacturatesterase）, and 4.3 U.L^-1 （polymethylgalacturonate lyase）, respectively, after 3 days at 180 ***^- 1 and 30℃. The crude pectinase shows significant effect to improve the yield and clarification of apple juice. Keywords Aspergillus niger, slurry-state fermentation, pectinase, Citrus changshan-huyou, apple juice

关键词： Aspergillus niger slurry-state fermentation pectinase Citrus changshan-huyou apple juice

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Evaluation of Water Transfer Capacity of Poplar with pectinase Treated under the Solar Interface Evaporation

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Journal of Renewable Materials 2023年第5期11卷 2265-2278页

作者： Wei Xiong Dagang Li Peixing Wei Lin Wang Qian Feng Nanjing Forestry University Nanjing210037China Jiangsu Vocational College of Agriculture and Forestry Jurong212400China

Poplar wood,which was used as the absorption material for the solar-driven interfacial evaporation,was treated for 3 days,6 days and 9 days with the pectinase,and then was simulated for photothermal evaporation test at one standard solar radiation intensity(1 kW⋅m^(−2)).The effects of pectinase treatment on cell passage and water migration capacity of poplars were analyzed by the mercury intrusion porosimetry,the scanning electron microscope and fractal *** was found that the pit membrane and the ray parenchyma cells of poplar wood were degraded and destroyed after pectinase *** with the untreated poplar wood,the evaporation rate of three sections of the specimen was *** the evaporation rate of radial and tangential direction was significantly *** the same time,based on the experimental data and fractal dimension deduction,fractal characteristics could be found in that the structure of poplars treated with *** porosity decreased with the increase of the fractal dimension in a certain *** was shown that it is feasible to evaluate solar-driven water migration capacity by using fractal theory.

关键词： pectinase poplar wood solar-driven water migration permeability fractal dimension

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Performance of a pectinase from Bacillus Subtilis WSHB04-02 Used in Bioscouring of Cotton Fabrics

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Journal of Donghua University(English Edition) 2007年第2期24卷 178-182页

作者：王强范雪荣陈坚 Key Laboratory of Eco-Textiles Ministry of Education Southern Yangtze University Key Laboratory of Industrial Biotechnology Ministry of EducationSouthern Yangtze University

A pectinase produced by Bacillus subtilis WSHB04-02 isolated from soil with lyase activity operating at alkaline pH was studied. The Michaelis-Menten kinetic parameters of this newly isolated pectinase on different substrates, such as citrus pectin and polygalacturonic acid (PGA), were determined, and pectin proved to be the most suitable substrate. The effects of temperature and pH on pectinase activity and stability were also investigated. The optimal temperature for pectinase was 55℃ with a stable range of 45℃-55℃. In general, pectinase was pH insensitive and the stable pH ranged from 8.6 to 10.0. Ultimately the bioscouring effects of cotton fabrics using this pectinase were evaluated and some promising results were obtained.

关键词： pectinase activity stability Uioscouring cotton

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Lucrative pectinase production by novel strain Pseudozyma sp. SPJ with statistical optimization techniques using agro-industrial residues

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Frontiers in Biology 2014年第4期9卷 317-323页

作者： Sampriya SHARMA Jitender SHARMA Rishi Pal MANDHAN Department of Biotechnology Kurukshetra University Kurukshetra 136119 India

Production of high titers of an alkaline, extracellular and thermo-tolerant pectinase by a newly isolated yeast Pseudozyma sp. SPJ was carried out under solid state fermentation. Citrus peel, the inexpensive agro-industrial residue used as substrate, was experienced to be unsurpassed. Response surface methodology was conducted to optimize the culture conditions for Pseudozyma sp. SPJ for hyper production of pectinase. Plackett Burman design was applied to identify the most effective culture variables. Out of nine variables studied, incubation time, moisture content and ammonium sulfate were detected as most important. A full factorial Central Composite Design was used to optimize the levels of these variables, which resulted in 17-fold increase （71.19 IU/g to 1215.66 IU/g dry substrate） in the enzyme yield. The results of analysis of variance and multiple regression analysis implies that the effect of incubation time （p 〈 0.000） and moisture content （p 〈 0.018） is more than ammonium sulfate. And also the interaction of moisture content with ammonium sulfate （p 〈 0.002） is more significant.

关键词： pectinase Pseudozyma sp. SPJ response surface methodology solid state fermentation

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Bio-reduction of Sulphur Dyes with Alkaline pectinase

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Journal of Chemistry and Chemical Engineering 2013年第10期7卷 930-941页

作者： Priyadarshi Jaruhar Jadu Nandan Chakraborty National Institute of Technology Jalandhar 144011 India

Sulphur dyes are invariably applied on cotton to produce deep shades at cheaper cost possessing all-round fastness properties except against chlorine. Being water insoluble, these dyes are reduced and solubilised with sodium sulphide at boil to develop affinity for cotton. Application of sulphide has generated global debate because of its eco-unfriendly technology of dyeing. In this work, attempts were made to substitute sodium sulphide with alkaline pectinase. Obtained results suggested the ability of the latter to cause effective reduction and solubilisation of sulphur dyes. Stability of reduction baths as well as colour fastness was also reported to be in line with those obtained using sodium sulphide.

关键词： Sulphur dye sodium sulphide pectinase dye strength stability color fastness

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Optimization of Wickerhamomyces anomalus Fermentation Conditions for pectinase Production Based on Wet Processing of Arabica Coffee in Yunnan Province

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Agricultural Science & Technology 2022年第3期23卷 30-39页

作者： ZHANG Shu-zhu MENG Jin XUE Xiao-ran KONG De-ting TIE Li-ping HUANG Jia-xiong QIN Shi-wen HE Fei-fei SUO Yu-kai School of Agriculture Yunnan UniversityKunming 650000PRC Key Laboratory of Chemistry in Ethnic Medicinal Resources State Ethnic Affairs Commission and Ministry of EducationYunnan Minzu UniversityKunming 650000PRC Key Laboratory of Synthetic Biology of Ethnic Medicinal Endophyte-derived Natural Products State Ethnic Affairs CommissionYunnan Minzu UniversityKunming 650000PRC Institute of Tropical and Subtropical Economic Crops Yunnan Academy of Agricultural SciencesPu’er 665000PRC

In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic Wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before ***, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.

关键词： Wickerhamomyces anomalus Coffee Wet processing pectinase Box–Behnken design

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Isolation of pectinase Producing Bacteria from the Rhizosphere of <i>Andrographis paniculata</i>Nees and 16S rRNA Gene Sequence Comparison of Some Potential Strains

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Advances in Microbiology 2019年第1期9卷 1-13页

作者： Md. Shahinur Kabir Tahera Tasmim Department of Botany Faculty of Biological Sciences Jahangirnagar University Dhaka Bangladesh

pectinases, the enzymes which break down pectic substances, have a wide range of applications in food, agriculture and environmental sectors. In the present study, attempts were made to isolate highly efficient pectinase producer from the rhizosphere of a medicinal plant, Andrographis paniculata Nees, known as the “King of bitters”. The total heterotrophic bacterial count of the rhizosphere soil of A. paniculata Nees ranged from 1.53 × 109 to 2.52 × 109 cfu/g. A total of 65 bacterial colonies were randomly selected from the nutrient agar plates, purified and assessed for pectinase activity. Out of the 65 isolates, 62 (95.38%) showed varying degree of pectinase activity in plate assay using pectin as a sole source of carbon. Among the pectinase producing strains, JBST36 showed best pectinase activity which is followed by the JBST22 and JBST27. Morphological characterization, biochemical tests and 16S rRNA gene sequencing were performed to identify the three most potential strains. Based on the morphological, biochemical and molecular data, JBST22 was identified as Bacillus flexus and the other two were identified as Bacillus subtilis. Furthermore, nucleotide sequences of the 16S rRNA gene of these 3 strains were compared and a phylogenetic tree was constructed. The study reveals that there are at least 66 base differences in the 16S rRNA gene sequences of B. flexus JBST22 and the B. subtilis JBST36.

关键词： 16S rRNA Gene Andrographis paniculata pectinase Rhizosphere

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Inducing and Screening of High Producing pectinase Aspergillus niger Strain

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Agricultural Science & Technology 2010年第4期11卷 22-23,29页

作者：屈二军李文建刘云肖陈兰英赵桢河南城建学院生物工程系河南平顶山467036

[Objective]The aim was to induce and screen the high producing pectinase Aspergillus niger Strain based on the original preservation strains.[Method]The original strain was induced by ultraviolet,and the highst enzyme activity and cultivated time were detected through the inspection of transparent circle and enzyme activity determination of flask fermentation.[Result] The enzyme activity of strain D1-4 achieved its highest after cultivated for 96 h in suitable conditions,which was 141.13 U/ml.[Conclusion] The induced strain D1-4 had the strong ability of producing pectinase.

关键词： Aspergillus niger pectinase Enzyme activity

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Study on Improving the Internal Quality of Tobacco Stems by Using pectinase

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Agricultural Science & Technology 2013年第9期14卷 1299-1302,1321页

作者：何邦华申晓锋陈兴汪显国巩效伟李水荣朱勇邱昌桂红云红河烟草(集团)有限责任公司技术中心云南昆明650231 云南同创检测技术股份有限公司云南昆明650106

[Objective] This study aimed to reduce the content of cell wall materials in tobacco stems and improve their internal quality. [Method] pectinase was used to decompose cell wall materials in tobacco stems, followed by determination of the contents of four cell wall materials, six routine chemical components, as well as aroma constituents. [Result] pectinase could effectively reduce the contents of cell wall materials in tobacco stems, with the largest decrease of 6.84%; after pectinase treatment,the content of reducing sugar in tobacco stems increased obviously, and the contents of total sugar, potassium ion, chloride ion and total nitrogen increased to varying degrees, of which the contents of potassium ion and reducing sugar displayed upward trends with the increase of pectinase concentration. pectinase treatment significantly increased the contents of Maillard reaction products, with the most increase of 67.2%;the contents of carotenoid degradation products, phenylalanine degradation products and neophytadiene all increased to varying extents, and the contents of both Maillard reaction products and phenylalanine degradation products revealed ascending trends with the increase of pectinase concentration. [Conclusion] pectinase treatment can effectively decompose cell wall materials in tobacco stems, improve routine chemical constituents, and increase the contents of aroma constituents.

关键词： pectinase Tobacco stems Cell wall materials Routine chemical components Aroma constituents

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