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Design and modelling of the full-feed peanut picking device with selfadaptive adjustable working clearance and feeding rate

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International Journal of Agricultural and Biological Engineering 2023年第6期16卷 97-106页

作者： Shenying Wang Baoliang Peng Huichang Wu Zhichao Hu Dawei Sun Yongwei Wang Mingzhu Cao College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China Nanjing Institute of Agricultural Mechanization Ministry of Agriculture and Rural AffairsNanjing 210014China Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province Hangzhou 310058China Key Laboratory of Agricultural Equipment for Hilly and Mountainous Areas in Southeastern China(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsHangzhou 310058China

To improve the declining performance of a full-feed peanut picking device or solve the mechanical failures that occur due to fluctuations in the feeding rate during operation,the 4HLJI-3000 peanut intelligent picking combine harvester,which is a picking device with a self-adaptive adjustment of the working clearance,was developed as the research object in this ***,the key components,such as the picking roller,concave plate sieve and clearance adjustment mechanism of the concave plate sieve,were designed and *** the force analysis of the concave plate sieve of the picking device,the mathematical model of the concave plate sieve displacement of the picking device and feeding rate was *** software system for monitoring,storing and analysing the concave plate sieve displacement of the picking device based on EasyBuilder Pro was designed,and the road monitoring test of displacement variation of concave plate sieve of the picking device and feeding rate was carried *** linear function,power function,exponential function,quadratic function,compound function,logarithmic function and cubic function fitting were used to perform regression analysis of the test results by using IBM SPSS *** results showed that the cubic function model had a higher fitting precision,and its determination coefficient was *** verification experiments were proposed,and the results showed that the established cubic function model had a good *** absolute deviation rate ranged from 0 to 4.83%,and the average deviation rate was 2.22%.The deviation rate increased with an increasing feeding *** field experiments also proved that there was a cubic function relationship between the feeding rate and concave plate sieve displacement,the measured concave plate sieve displacement deviation rate ranged from 0 to 6.19%,and the average deviation rate was 2.73%compared with the calculated *** study can provide a reference for the optimiza

关键词： agricultural machinery peanut picking device feeding rate concave plate sieve displacement EasyBuilder

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Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice

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Food Science and Human Wellness 2023年第3期12卷 765-773页

作者： Junjuan Wang Cui Zhou Shiwen Han Zainabu Majid Na Sun Huilian Che Key Laboratory of Precision Nutrition and Food Quality Key Laboratory of Functional DairyMinistry of EducationCollege of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China School of Public Health Capital Medical UniversityBeijing 100069China National Engineering Research Center of Seafood School of Food Science and TechnologyDalian Polytechnic UniversityDalian 116034China

The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions(Ca^(2+)) in peanut allergy remains unknown.C3 H/HeJ mice sensitized with peanut protein extract(PPE)were injected with anti-CD4 antibodies for 4 *** with PPE increased the specific immunoglobulin E(IgE),cytokine,histamine,and mMcp-1 levels,upregulated decorin(Dcn)expression,induced Ca^(2+) inflow in the spleen,and augmented the expression of the transcription factors GATA-3 and Foxp3,which resulted in Th2 and Treg cell ***,the Ca^(2+) levels were positively correlated with the histamine,interleukin(IL)-4,IL-5,and IL-13 levels,and negatively correlated with IL-10 ***,administration of anti-CD4 antibodies markedly alleviated allergic symptoms,activated T cells,and reduced Ca^(2+) inflow,cytokine,histamine,mMcp-1,and the IgHG3,CXCLI2,MMP2 and FABP4 *** results indicated that anti-CD4 antibodies can ameliorate PPE-induced allergy,which is probably related to the suppression of Ca^(2+) inflow,and inhibiting histamine,cytokine and IgHG3,CXCL12,MMP2,and FABP4,thus exerting a protective effect against PPEsensitized food allergy.

关键词： Calcium ions Anti-CD4 C3H/HeJ mice peanut Allergy

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Full-Length Transcriptome Analysis of Cultivated and Wild Tetraploid peanut

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 439-453页

作者： Danlei Song Xiaona Yu Yaoyao Li Xianheng Wang Xinyuan Cui Tong Si Xiaoxia Zou Yuefu Wang Minglun Wang Xiaojun Zhang Dry Farming Technology Key Laboratory of Shandong Province College of AgronomyQingdao Agricultural UniversityQingdao266109China

The high-quality genomes and large-scale full-length cDNA sequences of allotetraploid peanuts have been sequenced and released,which has accelerated the functional genomics and molecular breeding research of *** order to understand the difference in the transcriptional levels of wild and cultivated *** this study,we integrated of second-and third-generation sequencing technologies to sequence full-length transcriptomes in peanut ***9616 and its putative ancestor Arachis *** RNA extracted from six different tissues(i.e.,roots,stems,leaves,flowers,needles and pods)were sampled at 20 days after flowering.A total of 31,764 and 33,981 high-quality transcripts were obtained from Monticola and Pingdu9616,*** number of alternative splicing,the unit point mutation of variable adenylation,the number of open reading frames and the two-site mutation were identified in Pingdu9616 more than in Monticola,but the three-site mutation in Pingdu9616 was lower than in Monticola.1,691 LncRNAs,and 4,000 bp of maximum length of LncRNA was identified in Monticola and ***,comparative analysis between transcript data shown that 56 transcription factor families were involved in Monticola,and Pingdu9616 and the number of transcription factors in Pingdu9616 was higher than that in Monticola,the number of expressed genes estimated in flower,root,young pod and leaf organs was higher in Monticola than *** all,our study provided a valuable resource of large-scale full-length transcripts for further research of the molecular breeding and functional analysis of genes.

关键词： peanut second generation transcriptome full-length transcriptome

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Construction of EMS-Induced peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 537-557页

作者： Hao Chen Faqian Xiong Rilong Xu Xiangyu Chen Haifeng Zhong Yumei Zhang Xinlong Lan Hong Zhang Yuhua Chen Runfang Hu Guoqiang Lin Zhaoxiu Tang Institute of Crop Sciences Fujian Academy of Agricultural SciencesFujian Research Station of Crop Gene Resource&Germplasm EnhancementMinistry of Agriculture and Rural Affairs of People’s Republic of ChinaFujian Engineering Research Center for Characteristic Upland Crops BreedingFujian Engineering Laboratory of Crop Molecular BreedingFuzhou350013China Cash Crops Research Institute Guangxi Academy of Agricultural SciencesNanning530007China

peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing ***,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut *** broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this *** optimal EMS treatment conditions for the three peanut varieties were *** was found that the median lethal dose(LD50)of EMS treatment varied greatly among different ***,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and ***,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing *** genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were *** predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their *** of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of *** mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.

关键词： peanut EMS-induced mutant line pod-related traits re-sequencing pod constriction

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Adaptabilities of different harvesters to peanut plants after cutting stalks

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International Journal of Agricultural and Biological Engineering 2022年第2期15卷 93-101页

作者： Youqing Chen Gongpu Wang Jiangtao Wang Peng Zhang Bing Wang Zhichao Hu Nanjing Institute for Agricultural Mechanization Ministry of Agriculture and Rural AffairsNanjing 210014China Henan Nongyouwang Agricultural Equipment Technology Co. Ltd.Zhumadian 463100HenanChina

At present,there are no harvesters specifically adapted to process peanuts after cutting *** particular,methods for harvesting peanuts after cutting stalks have not been reported thus ***,to utilize peanut stalks as feed when harvesting plastic-film-grown peanuts,and to improve industry benefits,a three-stage harvesting method is proposed *** view of the three-stage harvesting method,the peanut stalks are cut before digging,with the remaining peanut plants being shorter,thereby increasing the peanut pod-stalk *** investigate the adaptabilities of existing harvesters in harvesting peanuts after cutting stalks,three types of peanut harvesters-the self-propelled pick-up combine harvester,trailed pick-up combine harvester,and peanut picker were used to conduct a comparative test on harvesting intact peanut plants and plants after cutting *** loss,breakage and impurity rates were used as evaluation *** loss rates of these three harvesters were 14.64%,16.44%and 1.33%;the breakage rates were 21.28%,21.92%and 20.00%,and impurity rates were 4.60%,8.76%and 9.06%.Analysis of variance showed that cutting stalks had a significant impact on the work qualities of the three harvesters(ppeanut combine harvesters could not be adapted to harvest peanut plants after cutting *** three harvesters had good adaptability to harvest peanut plants after cutting stalks,considering the breakage rate;however,based on the impurity rate,the three harvesters could not be adapted to harvest peanut plants after cutting *** losses of the two combine harvesters consisted mainly of dropped and missed picking,with the sum of the losses accounting for 99.87%and 97.99%of the total losses of the two harvesters,respectively;this suggests that the drum pickup of the combine harvesters could not adapt to harvesting the peanut after cutting *** breakage rates of the three harvester

关键词： agricultural machinery peanut peanut harvesting after cutting stalks peanut combine harvester peanut picker adaptability research

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Effects of Allelochemicals on Root Growth and Pod Yield in Response to Continuous Cropping Obstacle of peanut

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Phyton-International Journal of Experimental Botany 2023年第1期92卷 17-34页

作者： Zhaohui Tang Feng Guo Li Cui Qingkai Li Jialei Zhang Jianguo Wang Sha Yang Jingjing Meng Xinguo Li Ping Liu Shubo Wan Institute of Crop Germplasm Resources Shandong Academy of Agricultural SciencesJinan250100China Shandong Key Lab of Genetic Improvement Ecology and Physiology of CropsJinan250100China Scientific Observation and Experiment Station of Crop Cultivation in East China Ministry of Agriculture and Rural AffairsDongying257100China Shandong Agricultural Engineering University Jinan255300China Institute of Agricultural Resources and Environment Shandong Academy of Agricultural SciencesJinan250100China

Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly *** current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC *** with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with *** application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological *** allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut ***,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble *** of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root ***,allelochemicals significantly decreased the pod yield of peanut compared with *** results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.

关键词： peanut continuous cropping obstacle root growth pod yield

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Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature peanut (Arachis hypogaea L.) Cultivars

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Phyton-International Journal of Experimental Botany 2022年第12期91卷 2619-2631页

作者： Abraham Lamboro Xiao Han Songnan Yang Xueying Li Dan Yao Baixing Song Jun Zhang Department of Crop Genetics and Breeding College of AgronomyJilin Agricultural UniversityChangchun130118China Department of Biochemistry and Molecular Biology College of Life ScienceJilin Agricultural UniversityChangchun130118China

Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits.A combination of 6-benzylaminopurine(BAP)and thidiazuron(TDZ)plant growth regulators was applied to evaluate shoot regeneration capacity whereasα-naphthalene acetic acid(NAA)combination with 6-benzylaminopurine(BAP),and 2,4-dichlorophenoxyacetic acid(2,4-D)with 6-benzylaminopurine were tested to optimize root induction for two peanut *** result showed combination(BAP with TDZ)was found to be effective in promoting *** highest shoot regeneration frequency(93%)was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average regeneration frequency(87%)was achieved in a medium containing combinations of 2 mg/L BAP with 1 mg/L *** shooting rate increased for both cultivars as the concentrations of BAP increased and TDZ *** highest rooting rate(93%)was obtained on a medium supplemented with 3.5 mg/L NAA with 2.5 mg/L BAP for both *** rooting rate increased as the concentration of auxin to cytokinin ratio *** maximum rooting rate(83%)was obtained on MS medium supplemented with 0.3 mg/L 2,4-D with 0.2 mg/L BAP for the cultivar *** result indicated that BAP with NAA was much better than BAP with 2,4-D in rooting ***,the protocol developed was genotype independent and effective for peanut tissue culture.

关键词： Auxin cotyledonary node cytokinins in vitro peanut

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Evaluation of Beta-Lactam Antibiotics on the Regeneration of peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2489-2501页

作者： Abraham Lamboro Songnan Yang Xueying Li Dan Yao Jun Zhang College of Agronomy Jilin Agricultural UniversityChangchun130118China College of Life Science Jilin Agricultural UniversityChangchun130118China Department of Biology College of Natural and Computational ScienceWachemo UniversityHossana667Ethiopia

The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut *** culture media contained 4 mg/L 6-benzylaminopurine(BAP)as the main growth *** concentrations(100–600 mg/L)of cefotaxime,carbenicillin,and timentin were applied in the culture *** all the tested media,there were no significant differences in the shoot induction as compared to the ***,little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation *** shoot elongation medium,shoots turned brownish and partly died at higher concentrations where shooting rates were not affected by the *** cefotaxime,timentin,and carbenicillin-containing media,levels of antibiotics greater than 400,300,and 200 mg/L,respectively resulted in the brown coloration of ***,the mean shoot number and shoot weight significantly decreased as their dosage *** results indicate that maximum levels of antibiotics have an adverse effect on the growth and development of ***,cefotaxime(100–300 mg/L)and timentin(100–300 mg/L)will be sufficient in controlling Agrobacterium growth in the culture media with the least phytotoxic effect on the peanut plants.

关键词： peanut antibiotics shoot cotyledonary node regeneration

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Development of the peanut precision fertilization control system based on threshold velocity algorithm

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International Journal of Agricultural and Biological Engineering 2022年第5期15卷 116-122页

作者： Yuqi Fan Yan Yu Shuqi Shang Tianying Gu Yanrui Hu Linsong Diao Ruchao Ge Xing Zhang College of Mechanical and Electrical Engineering Qingdao Agricultural UniversityQingdao 266109ShandongChina

In view of the fact that the current ground wheel velocimetry of the peanut precision fertilizer control system cannot solve the phenomenon of ground wheel slippage,and signal interference and delay loss cannot be excluded by BeiDou positioning velocimetry,a set of peanut precision fertilizer control system was designed based on the threshold speed *** system used STM32F103ZET6 microcontroller as the main controller,and touch screen for setting the operating parameters such as operating width,fertilizer type,and fertilizer application *** threshold speed algorithm combined with BeiDou and ground wheel velocimetry was adopted to obtain the forward speed of the tractor and adjust the speed of the DC drive motor of the fertilizer applicator in real time to achieve precise fertilizer ***,through the threshold speed algorithm test,the optimal value of the length N of the ground wheel speed measurement queue was determined as 3,and the threshold of the speed variation coefficient was set to 4.6%.Then,the response performance of the threshold speed algorithm was verified by comparative test with different fertilization amounts(40 kg/hm^(2),50 kg/hm^(2),60 kg/hm^(2),70 kg/hm^(2))under two speed acquisition methods of ground wheel speed measurement and threshold speed algorithm(combination of Beidou single-point speed and ground wheel speed measurement)in different operation speeds(3 km/h,4 km/h,5 km/h).The response performance test results showed that the average value of the velocimetry delay distance of the BeiDou single-point positioning velocimetry method was 0.58 m,while the average value of that with the threshold velocity algorithm was 0.27 m,which decreased by 0.31 m and indicated more accurate with the threshold velocity *** field comparison test for fertilizer application performance turned out an over 96.08%accuracy rate of fertilizer discharge by applied with the threshold speed algorithm,which effectively avoided the ina

关键词： peanut precision fertilizer planter BeiDou velocimetry ground wheel velocimetry threshold speed algorithm control system

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Quantitative Trait Loci(QTL)Mapping and Marker Analysis of Fatty Acids in peanut

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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2577-2589页

作者： Xiao Han Songnan Yang Xueying Li Qiulin Wu Yongyi Xing Jun Zhang Fenglou Ling College of Agronomy Jilin Agricultural UniversityChangchun130118China

peanut,with high oil content,has been a major oil and food crop *** compositions of the fatty acids are the common factors in determining the oil *** the present study,an F2 segregated population with 140 individuals derived from the cross of Weihua8(a cultivar)and 12L49(a line with high oleic acid concentration)was used to construct a genetic map and conduct QTL mapping analysis.A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR *** the 103 SSR loci,a genetic linkage map,covering a total length of 3592.35 cM of the whole peanut genome,was *** on the genetic map,sixteen QTLs located on nine linkage groups related to peanut fatty acids were finally *** them,four QTLs were detected associated with various traits simultaneously,which showed genetic stability in relation to fatty acids of *** for the QTLs for oleic acid,linoleic acid,and linolenic acid,three novel QTLs for arachidic acid and behenic acid were also *** QTLs might be helpful for further fine mapping analysis and marker-assisted selection of fatty acids in peanut.

关键词： peanut fatty acids simple sequence repeat genetic linkage map QTL mapping

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