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Effect of Vacuum Packaging on Storage Quality of peanut

Asian Agricultural Research 2016年第1期8卷 72-74,78页

作者： Anjian WANG Shuaiping GAO Guangrui TIAN Lina LIU Institute of Agricultural Product Processing Henan Academy of Agricultural Sciences

The purpose of the study is to investigate the effect of vacuum packaging on storage quality of peanut. The acid value,peroxide value,relative conductivity,mildew rate and germination rate of peanut were examined in both vacuum-packed bags and control packaging *** results showed that the acid value,peroxide value,relative conductivity and mildew rate of peanut were maintained at a lower level and germination rate was maintained at a higher level in vacuum-packed bags than in control packaging bags. The storage quality of peanut in vacuum-packed bags was better than in control packaging bags. The order of different vacuum degree of vacuum-packed bags from good to bad according to storage quality of peanut was- 0. 06 MPa >- 0. 09 MPa >- 0. 03 MPa,and- 0. 06 MPa was the most beneficial vacuum degree for the peanut storage.

关键词： peanut Vacuum packaging Storage Quality

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High-density genetic map construction and identification of QTLs controlling oil content in peanut using SLAF-seq and SSRs

High-density genetic map construction and identification of ...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： ZHANG Sheng-zhong HU Xiao-hui MIAO Hua-rong YANG Wei-qiang CHEN Jing Shandong peanut Research Institute

Cultivated peanut,*** L.,is an globally important oil and food ***-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative *** this context,a recombinant inbred line(RIL) of 181 lines was developed by crossing Huayu 36 and Gaoyou *** developed433 679 high-quality SLAFs,of which 890 821 SNPs were detected by using GATK and samtools methods.39 600 SLAFs were encoded and grouped into different segregation patterns.A high-resolution genetic map containing 3 199 markers(36 SSRs and 3 163 SNPs) on 20 linkage groups(LGs) spanning 1 236.50 cM was constructed for *** average distance between adjacent markers was 0.39 *** on phenotyping in two environments,QTLs for oil content were identified and positioned on linkage groups A01,A08,A10,B07 and B08,with PVE varied from 5.70%to 20.48%.This map exhibits high resolution and accuracy,which will facilitate QTL discovery for essential agronomic traits in peanut.

关键词： SLAF peanut genetic map QTL oil content

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Deep sequencing analysis of the transcriptomes of peanut aerial and subterranean young pods identifies candidate genes related to early embryo abortion

Deep sequencing analysis of the transcriptomes of peanut aer...

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第十四届全国植物基因组学大会

作者： Xiaoping Chen Xuanqiang Liang Crops Research Institute Guangdong Academy of Agricultural Sciences(GAAS)

The failure of peg penetration into the soil leads to seed abortion in peanut. Knowledge of genes involved in these processes is comparatively ***,we used RNA-seq to gain insights into transcriptomes of aerial and sub... 详细信息

关键词： peanut aerial and subterranean pod transcriptome RNA sequencing

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Identification and functional analysis of KCS gene family in peanut(Arachis hypogaea L.)

Identification and functional analysis of KCS gene family in...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： HUAI Dong-xin XUE Xiao-meng ZHANG Zhao-hua WANG Zhi-hui YAN Li-ying WAN Li-yun JIANG Hui-fang LEI Yong LIAO Bo-shou Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of AgricultureOil Crops Research Institute of Chinese Academy of Agricultural Sciences

β-ketoacyl-CoA synthase(KCS) is the key enzyme for biosynthesis of very long chain fatty acids(VLCFA) in *** this study,a total of 33 KCS genes were identified and *** of them were expressed in 22 tissues,and most of them exhibited expressing tissue ***13,AhKCS15,AhKCS20,AhKCS21,AhKCS27 and AhKCS31 were highly expressed in leaf,while AhKCS1,AhKCS13,AhKCS18,AhKCS20,AhKCS27 and AhKCS30 were highly expressed in *** every KCS gene was expressed in *** developing seed,AhKCS1,AhKCS6,AhKCS10,AhKCS15,AhKCS18,AhKCS25,AhKCS30 and AhKCS31 were most abundant ***,only AhKCS15 exhibited *** 8 KCS genes were cloned and heterologously expressed in yeast,*** 8 KCS genes could produce VLCFAs in yeast,but different substrate preferences of them were ***10,AhKCS15 and AhKCS31 only added 2 carbons to oleic acid and formed arachidonic acid,but other 3 copies added 2 more carbons to oleic acid and generated nervonic *** results indicated that KCS genes in peanut played different role in different tissues at different developing *** identified KCS genes provide valuable information for improvement of oil quality and enhancement of defense in peanut.

关键词： β-ketoacyl-CoA synthase peanut expression profie function

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Current Situations and Development Strategies for Integration of peanut Agronomy and Agricultural Machinery in China

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Asian Agricultural Research 2015年第12期7卷 77-81,89页

作者： Zhengfeng WU Junhua LIU Hao FENG Dianxu CHEN Caibin WANG Shandong peanut Research Institute Binzhou University

The mechanization level of peanut production is the bottleneck restricting the development of peanut production in China,while integration of agronomy and agricultural machinery is an essential approach for improving mechanization level of peanut production. This paper elaborated requirements of peanut agronomy for agricultural machinery from peanut planting modes,land cultivation,sowing,harvesting,picking,and shelling,etc. Besides,it discussed requirements of agricultural machinery for agronomy from peanut seed quality,variety characteristics,planting modes,and soil condition. In addition,it analyzed existing problems and restricting factors of integration of peanut agronomy and agricultural machinery. Finally,it came up with recommendations for development strategies including increasing government fund input,optimizing industrial distribution,and multidisciplinary joint research.

关键词： peanut Agronomy Agricultural machinery Simplified cultivation Development strategies

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Identification of a major and consensus quantitative locus for oil content on chromosome A08 in peanut using high dense genetic map

Identification of a major and consensus quantitative locus f...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： LIU Nian GUO Jian-bin ZHOU Xiao-jing LIAO Bo-shou JIANG Hui-fang Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences

Enhancing oil content is an important breeding objective in ***-resolution mapping of QTLs with linked markers could facilitate marker-assisted selection in breeding for target *** this study,double-digest restriction-site associated DNA sequencing(ddRAD-seq) was performed to construct a genetic map for a recombined inbred line(RILs) population(Xuhua 13 x Zhonghua 6).The high dense genetic map(HDGM) contained 2 595 loci,spanning a length of 2 465.62 cM with average distance of 0.95 cM/*** QTLs were identified on five linkage groups(LGs) for oil content,including a major and stable QTL(q0 CA08.1)on chromosome A08 with 10.14%-27.19%phenotypic variation explained(PVE).The physical interval of qOCA08.1 was further delimited into an approximately 0.8 Mb genomic region where three genes coding enzyme and transcription factors related to oil biosynthesis had been *** to the flanked SNP loci,the marker(SNPOCA08) associated with oil content was developed and validated in diverse peanut *** QTLs identified in the present study could be further dissected for gene discovery,and the tightly linked marker for oil content would be useful in marker assisted breeding in peanut.

关键词： ddRAD-seq high dense genetic map QTL for oil content peanut

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AhRLK1,a CLAVATA1-like leucine-rich repeat receptor-like kinase of peanut,confers increased resistance to bacterial wilt in tobacco

AhRLK1,a CLAVATA1-like leucine-rich repeat receptor-like kin...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： ZHANG Chong CHEN Hua ZHUANG Rui-rong DENG Ye CAI Tie-cheng WANG Shuai-yin LIU Qin-zheng TANG Rong-hua SHAN Shi-hua PAN Rong-long ZHUANG Wei-jian State key laboratory of ecological pest control for Fujian and Taiwan crops Fujian Agriculture and Forestry University Key Laboratory of Crop Molecular and Cell Biology Fujian Agriculture and Forestry University Cash Crops Research Institute Guangxi Academy of Agricultural Sciences Shandong peanut Research Institute Department of Life Science and Institute of Bioinformatics and Structural Biology College of Life ScienceNational Tsing Hua University

Bacterial wilt caused by Ralstonia solanacearum is a devastating disease that infects hundreds of plant *** factors involved in disease resistance and pathogenesis remain poorly *** up regulated and leucine-rich repeat receptor-like kinase characterized as CLAVATA1 and named AhRLK1 was obtained by microarray analysis in response to *** in *** contained presumably,a signal peptide,ten leucine-rich repeat(LRR) domains and conserved motifs of intracellular *** subcellular localization,the AhRLKl protein was visualized only in plasma *** inoculation with ***,AhRLK1 was constantly up regulated in the susceptible variety Xinhuixiaoli but showed little changed in the resistant cultivar Yueyou *** hormones,including salicylic acid,abscisic acid,methyl jasmonate and ethephon induced expression,but expression was completely down regulated under cold and drought *** overexpression provoked a hypersensitive response(HR) in Nicotiana benthamiana following ***,the resistance to *** increased significantly in transgenic overexpression *** contrast,most representative defense-responsive genes in defence related pathways such as NtHIN1,NtPR2,NtLOX1 and NtACS6,were considerably up regulated in the AhRLK1 transgenic ***,EDS1 and PAD4 in R gene signal were also up regulated in transgenic plants,but NDR1 and NPR1 genes were down ***,we suggest that AhRLK1 increases the defense response to *** via HR and hormone defense signalling,associated with EDS1 pathway of R gene *** results provide new understanding of CLV1 function and will contribute to genetic enhancement of peanut.

关键词： peanut bacterial wilt microarray analysis AhRLK1

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Rapid and Non-destructive Prediction of Protein Content in peanut Varieties Using Near-infrared Hyperspectral Imaging Method

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Grain & Oil Science and Technology 2018年第1期1卷 40-43页

作者： WANG Yijie CHENG Junhu School of Food Science and Engineering South China University of Technology

This study was undertaken to investigate the feasibility of near-infrared(NIR) hyperspectral imaging(1 000–2 500 nm) for non-destructive and quantitative prediction of protein content in peanut kernels. Partial least squares regression(PLSR) calibration model was established between the spectral data extracted from the hyperspectral images and the reference measured protein content values, with the coefficient of determination of prediction(R_P^2) of 0.885 and root mean square error of prediction(RMSEP) of 0.465%.Regression coefficients(RC) from PLSR analysis were used to identify the most essential wavelengths that had the greatest influence on changes in the protein content. Eight optimal wavelengths were selected by RC and its corresponding simplified RC-PLSR prediction model was also obtained, showing better performance with a higher R_P^2 of 0.870 and a lower RMSEP of 0.494%. The results indicate that hyperspectral imaging with PLSR analysis can be used as a rapid and non-destructive method for predicting protein content in peanut.

关键词： Hyperspectral imaging peanut Non-destructive Protein content Wavelength selection

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Drought stress tolerance strategies revealed by physiology and RNA-Seq in peanut

Drought stress tolerance strategies revealed by physiology a...

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第十九届中国作物学会学术年会

作者： Jingyao Ren He Zhang Xin Ai Rengyuan Li Haiqiu Yu Shenyang Agriculture University College of agronomy

【Background】peanut(Arachis hypogaea. L.), an important cash crop and oil crop, plays an increasingly prominent role in agricultural production developing and supply in recent years. As the fourth largest peanut producing area in China, Northeast China has been recognized as a high-quality peanut production base due to its lower risk of aflatoxin pollution, and its planting area and yield have been greatly developed.【Materials and methods】 In this study, peanut seedlings were cultured in indoor potted till to the four-leaf stage, and 20% PEG6000 was used to simulate drought stress. Leaves were sampled within 0 h to 24 h of drought treatment for the determination of physiological indicators and high-throughput transcriptome sequencing, to explore the physiological mechanism of peanut response to drought stress and identify key genes in peanut response to drought stress.【Results】Under drought stress, hydrogen peroxide content of plants increased and accumulated in sensitive varieties with a higher level, which increased by 2.4 times compared with control. Meanwhile, the anti-superoxide anion activity in drought-sensitive varieties decreased significantly, but it kept stronger in drought-tolerant varieties under drought stress. The variation coefficient analysis showed that the protective enzyme activity of drought-tolerant cultivars ranged from 8.4% to 22.40%, while in sensitive cultivars, it ranged from 9.3% to 53.36%. High-throughput transcriptome results showed that under drought stress, a total of 14126 differentially expressed genes were identified in drought-sensitive cultivars, among which 5511 genes were continuously differentially expressed, and these genes were significantly enriched in photosynthetic antenna protein, photosynthesis, carbon metabolism pathways. Among the drought-tolerant varieties, 8982 differentially expressed genes were identified, among which 2360 genes were continuously differentially expressed under drought stress. These genes were signi

关键词： peanut drought RNA-seq physiology

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Cloning and Analysis of a NBS-LRR Disease Resistance Gene candidate PnAG1 from peanut(Arachis hypogaea L.)

Cloning and Analysis of a NBS-LRR Disease Resistance Gene ca...

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中国作物学会50周年庆祝会暨2011年学术年会

作者：单世华万书波山东省花生研究所山东省农科院

peanut(Arachis hypogaea L.) is one of the four major oil crops in the world,and also the highest amount of export oil crops in ***,pests and diseases,in particular aflatoxin contamination in peanut have become more common,often resulting in the deterioration of yield and *** aflatoxin contamination has been identified by the peanut industry as a serious issue in food safety and human health

关键词： bioinformatics NBS-LRR peanut real-time fluorescence quantitative PCR

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