检索结果-南通市图书馆

Molecular Plant 2024年第5期17卷 699-724页

作者： Antonio Molina Lucıa Jorda Miguel Angel Torres Marina Martın-Dacal Diego Jose Berlanga Patricia Fernandez-Calvo Elena Gomez-Rubio Sonsoles Martın-Santamarıa Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA/CSIC)Campus de Montegancedo UPMPozuelo de Alarcon(Madrid)Spain Departamento de Biotecnologıa-Biología Vegetal Escuela Técnica Superior de Ingenierıa AgronomicaAlimentarıa y de BiosistemasUPMMadridSpain Centro de Investigaciones Biologicas Margarita Salas Consejo Superior de Investigaciones Científicas(CSIC)Ramiro de Maeztu 928040 MadridSpain Misión Biológica de Galicia Consejo Superior de Investigaciones Científicas(CSIC)Campus de Montegancedo UPMPozuelo de Alarcón(Madrid)Spain

Beyond their function as structural barriers,plant cell walls are essential elements for the adaptation of plants to environmental *** walls are dynamic structures whose composition and integrity can be altered in response to environmental challenges and developmental *** wall changes are perceived by plant sensors/receptors to trigger adaptative responses during development and upon stress *** cell wall damage caused by pathogen infection,wounding,or other stresses leads to the release of wall molecules,such as carbohydrates(glycans),that function as damage-associated molecular patterns(DAMPs).DAMPs are perceived by the extracellular ectodomains(ECDs)of pattern recognition receptors(PRRs)to activate pattern-triggered immunity(PTI)and disease ***,glycans released from the walls and extracellular layers of microorganisms interacting with plants are recognized as microbe-associated molecular patterns(MAMPs)by specific ECD-PRRs triggering PTI *** number of oligosaccharides DAMPs/MAMPs identified that are perceived by plants has increased in recent ***,the structural mechanisms underlying glycan recognition by plant PRRs remain ***,this knowledge is mainly focused on receptors of the LysM-PRR family,which are involved in the perception of various molecules,such as chitooligosaccharides from fungi and lipo-chitooligosaccharides(i.e.,Nod/MYC factors from bacteria and mycorrhiza,respectively)that trigger differential physiological ***,additional families of plant PRRs have recently been implicated in oligosaccharide/polysaccharide *** include receptor kinases(RKs)with leucine-rich repeat and Malectin domains in their ECDs(LRR-MAL RKs),Catharanthus roseus receptor-LIKE KINASE 1-LIKE group(CrRLK1L)with Malectin-like domains in their ECDs,as well as wall-associated kinases,lectin-RKs,and *** characterization of structural basis of glycans recogniti

关键词： Arabidopsis thaliana cell wall damage-associated molecular patterns DAMPs disease resistance glycans oligosaccharides pattern-triggered immunity PTl pattern recognition receptor PRRs

pattern recognition receptors in zebrafish provide functional and evolutionary insight into innate immune signaling pathways

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2017年第1期14卷 80-89页

作者： Yajuan Li Yuelong Li Xiaocong Cao Xiangyu Jin Tengchuan Jin Laboratory of Structural Immunology CAS Key Laboratory of Innate Immunity and Chronic Disease CAS Center for Excellence in Molecular Cell Science School of Life Sciences and Medical Center University of Science and Technology of China Hefei Anhui 230027 China

pattern recognition receptors （PRRs） and their signaling pathways have essential roles in recognizing various components of pathogens as well as damaged cells and triggering inflammatory responses that eliminate invading microorganisms and damaged cells. The zebrafish relies heavily on these primary defense mechanisms against pathogens. Here, we review the major PRR signaling pathways in the zebrafish innate immune system and compare these signaling pathways in zebrafish and humans to reveal their evolutionary relationship and better understand their innate immune defense mechanisms.

关键词： caspases innate immune system NOD-like receptors pattern recognition receptor RIG-I-like receptors Toll-like receptors zebrafish

Roles of pattern recognition receptors in diabetic nephropathy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第3期21卷 192-203页

作者： Zhi-feng ZHOU Lei JIANG Qing ZHAO Yu WANG Jing ZHOU Qin-kai CHEN Jin-lei LV The First Clinical Medical College of Nanchang University Nanchang 330006China Department of Nephrology the First Affiliated Hospital of Nanchang UniversityInstitute of Molecular Immunology of Kidney Disease of Nanchang UniversityNanchang 330006China

Diabetic nephropathy(DN)is currently the most common complication of *** is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic *** pathogenesis of DN is extremely complex and has not yet been clarified;however,in recent years,increasing evidence has shown the important role of innate immunity in DN *** recognition receptors(PRRs)are important components of the innate immune system and have a significant impact on the occurrence and development of *** this review,we classify PRRs into secretory,endocytic,and signal transduction PRRs according to the relationship between the PRRs and subcellular *** can recognize related pathogen-associated molecular patterns(PAMPs)and danger-associated molecular patterns(DAMPs),thus triggering a series of inflammatory responses,promoting renal fibrosis,and finally causing renal *** this review,we describe the proposed role of each type of PRRs in the development and progression of DN.

关键词： Diabetic nephropathy Innate immunity pattern recognition receptor Pathogenesis

Expansion of pathogen recognition specificity in plants using pattern recognition receptors and artificially designed decoys

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2017年第8期60卷 797-805页

作者： Lifan Sun Jun Qin Kailun Wang Jie Zhang State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Pathogen/microbe-associated molecular patterns(PAMPs/MAMPs) are recognized by plant pattern recognition receptors(PRRs)localized on the cell surface to activate immune *** PAMP-triggered immunity(PTI) confers resistance to a broad range of pathogenic microbes and,therefore,has a great potential for genetically engineering broad-spectrum resistance by transferring PRRs across plant *** effectors secreted by phytopathogens often directly target and inhibit key components of PTI signaling pathways via diverse biochemical *** some cases,plants have evolved to produce decoy proteins that mimic the direct virulence target,which senses the biochemical activities of pathogenic *** kind of perception traps the effectors of erroneous targeting and results in the activation of effector-triggered immunity(ETI) instead of suppressing *** mechanism suggests that artificially designed decoy proteins could be used to generate new recognition specificities in a particular *** this review,we summarize recent advances in research investigating PAMP recognition by PRRs and virulence effector surveillance by decoy *** expansion of recognition specificities,conferred by the transgenic expression of EF-Tu receptor(EFR) and AvrPphB susceptible 1(PBS1) decoys,has highlighted the considerable potential of PRRs and artificially designed decoys to expand plant resistance spectra and the need to further identify novel PRRs and decoys.

关键词： pattern recognition receptor effector decoy PAMP-triggered immunity resistance

Plasma Membrane Localization and Potential Endocytosis of Constitutively Expressed XA21 Proteins in Transgenic Rice

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2010年第5期3卷 917-926页

作者： Fang Chen Ming-Jun Gao Yan-Song Miao Yue-Xing Yuan Mu-Yang Wang Qun Li Bi-Zeng Mao Li-Wen Jiang Zu-Hua He National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Present address: Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06511 USA Department of Biology and Molecular Biotechnology Program The Chinese University of Hong Kong Shatin New Territories Hong Kong China Present address: Department of Emergency Medicine Thomas Jefferson University Philadelphia PA 19107 USA Biotechnology Institute Zhejiang University Hanghzou 310029 China

The rice pattern recognition receptor （PRR） XA21 confers race-specific resistance in leaf infection by bacterial blight Xathornonas oryzae pv. oryzae （Xoo）, and was shown to be primarily localized to the endoplasmic reticulum （ER） when expressed with its native promoter or overexpressed in the protoplast. However, whether the protein is still ER- localization in the intact cell when overexpressed remains to be identified. Here, we showed that XA21, its kinase-dead mutant XA21PK736EP and the triple autophosphorylation mutant XA21PS686AJT688AJS699A GFP fusions were primarily localized to the plasma membrane （PM） when overexpressed in the intact transgenic rice cell, and also localized to the ER in the transgenic protoplast. The transgenic plants constitutively expressing the wild-type XA21 or its GFP fusion displayed racespecific resistance to Xoo at the adult and seedling stages. XA21 and XA21PK736EP could be internalized probably via the SCAMP-positive early endosomal compartment in the protoplast, suggesting that XA21 might be endocytosed to initiate resistance responses during pathogen infection. We also established a root infection system and demonstrated that XA21 also mediated race-specific resistance responses to Xoo in the root. Our current study provides an insight into the nature of the XA21-mediated resistance and a practical approach using the root cell system to further dissect the cellular signaling of the PRR during the rice-Xoo interaction.

关键词： Rice XA21 pattern recognition receptor bacterial bight cellular localization internalization root infection race-specific resistance.

Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 2011年第5期29卷 942-951页

作者：罗展张继泉李富花张晓军柳承璋相建海 Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Acting as one of the pattern recognition receptors （PRRs）, C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene （named LvLecl） was cloned from the shrimp Litopenaeus vannamei, The ORF of LvLecl is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain （CRD） at the C-terminal. LvLecl was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus （WSSV）. RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic ＂EPN＂（Glu-Pro-Ser） or ＂QPD＂（Gin-Pro-Asp） motif, the recombinant LvLecl, expressed in Escherichia coli BL21 （DE3）, could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.

关键词： Litopenaeus vannamei innate immune pattern recognition receptor C-type lectin RNAinterference (RNAi) recombinant expression

DC-SIGN and Immunoregulation

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2006年第4期3卷 279-283页

作者： Tong Zhou Yongxi Chen Li Hao Yanyun Zhang Department of Nephrology Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200025 China Shanghai Institute of Immunology and Institute of Health Sciences Shanghai Institute of Biological Sciences Shanghai Jiao Tong University Shanghai 200025 China

Dendritic cells （DCs） are known to be the most powerful professional antigen-presenting cells so far. It could activate primary immune response, and also downregulate immune response. DCs have a unique character of immunoregulation. DC-SIGN, a molecule designated as CD209, is one member of the C-type lectin superfamily. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. DC-SIGN has become hotspot of recent studies because of its important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. These studies on DC-SIGN involved in primary and secondary immune response and relevant mechanism will certainly provide us with a new method in treating and preventing certain diseases.

关键词： DC DC-SIGN pattern recognition receptor immunoregulation

The macrophage soluble receptor AIM/Api6/CD5L displays a broad pathogen recognition spectrum and is involved in early response to microbial aggression

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2014年第4期11卷 343-354页

作者： Vanesa G. Martinez Cristina Escoda-Ferran Ines Tadeu Simees Satoko Arai Marc Orta Mascaro Esther Carreras Mario Martinez-Florensa Jose Yelamos Toru Miyazaki Francisco Lozano Grup d'Immunoreceptors del Sistema Innat i Adaptatiu Institut d'Investigacions Biomediques August Pii Sunyer (IDIBAPS) Barcelona Spain Center for Disease Biology and Integrative Medicine Faculty of Medicine University of Tokyo Tokyo Cancer Research Program Hospital del Mar Medical Research Institute and Department of Immunology Hospital del Mar Barcelona Spain Servei d'Immunologia Centre de DiagnOstic Biomedic Hospital Clinic Barcelona Barcelona Spain Departament de Biologia Cellular Immunologia i Neurociencies Facultat de Medicina Universitat de Barcelona Barcelona Spain

Apoptosis inhibitor of macrophages （AIMs）, a homologue of human Spa, is a mouse soluble member of the scavenger receptor cysteine-rich superfamily （SRCR-SF）. This family integrates a group of proteins expressed by innate and adaptive immune cells for which no unifying function has yet been described. Pleiotropic functions have been ascribed to AIM, from viability support in lymphocytes during thymic selection to lipid metabolism and anti-inflammatory effects in autoimmune pathologies. In the present report, the pathogen binding properties of AIM have been explored. By using a recombinant form of AIM （rAIM） expressed in mammalian cells, it is shown that this protein is able to bind and aggregate Gram-positive and Gram-negative bacteria, as well as pathogenic and saprophytic fungal species. Importantly, endogenous AIM from mouse serum also binds to microorganisms and secretion of AIM was rapidly induced in mouse spleen macrophages following exposure to conserved microbial cell wall components. Cytokine release induced by well-known bacterial and fungal Toll-like receptor （TLR） ligands on mouse splenocytes was also inhibited in the presence of rAIM. Furthermore, mouse models of pathogen-associated molecular patterns （PAMPs）-induced septic shock of bacterial and fungal origin showed that serum AIM levels changed in a time-dependent manner. Altogether, these data suggest that AIM plays a general homeostatic role by supporting innate humoral defense during pathogen aggression.

关键词： innate immunity pattern recognition receptor scavenger receptor

Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2015年第7期57卷 641-652页

作者： Fen Lu Huiqin Wang Shanzhi Wang Wendi Jiang Changlin Shan Bin Li Jun Yang Shiyong Zhang Wenxian Sun Department of Plant Pathology China Agricultural University Key Laboratory in Plant Pathology Ministry of AgricultureChina Agricultural University State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of AgricultureInstitute of BiotechnologyZhejiang University Rice Research Institute Shandong Academy of Agricultural Science

The elongation factor Tu （EF-Tu） receptor （EFR） in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants （Oryza sativa L. ssp. japonica cv. Zhonghua 17） and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We＇demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa- tion in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig- gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expressionlevel. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

关键词： Bacterial disease resistance elongation factor Tu receptor interspecific gene transfer pattern recognition receptor rice