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An in vitro synthetic biology platform for emerging industrial biomanufacturing:Bottom-up pathway design

Synthetic and Systems Biotechnology 2018年第3期3卷 186-195页

作者： Ting Shi Pingping Han Chun You Yi-Heng P.Job Zhang Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences32 West 7th AvenueTianjin Airport Economic AreaTianjin300308China

Although most in vitro(cell-free)synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products,in vitro synthetic biology platform,which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes,has been proposed for low-cost biomanufacturing of bioenergy,food,biochemicals,and nutraceuticals.In addition to the most important advantage-high product yield,in vitro synthetic biology platform features several other biomanufacturing advantages,such as fast reaction rate,easy product separation,open process control,broad reaction condition,tolerance to toxic substrates or products,and so on.In this article,we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks(thermoenzymes and/or immobilized enzymes)to building modules(e.g.,enzyme complexes or multiple enzymes as a module)with specific functions.With development in thermostable building blocks-BioBricks and modules,the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities.

关键词： Biomanufacturing Coenzyme regeneration pathway design In vitro synthetic biology

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Recent advances in microbial production of phenolic compounds

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Chinese Journal of Chemical Engineering 2021年第2期34卷 54-61页

作者： Xinxiao Sun Xianglai Li Xiaolin Shen Jia Wang Qipeng Yuan State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China

Phenolic compounds(PCs)are a group of compounds with various applications in nutraceutical,pharmaceutical and cosmetic industries.Their supply by plant extraction and chemical synthesis is often limited by low yield and high cost.Microbial production represents as a promising alternative for efficient and sustainable production of PCs.In this review,we summarize recent advances in this field,which include enzyme mining and engineering to construct artificial pathways,balance of enzyme expression to improve pathway efficiency,coculture engineering to alleviate metabolic burden and side-reactions,and the use of genetic circuits for dynamic regulation and high throughput screening.Finally,current challenges and future perspectives for efficient production of PCs are also discussed.

关键词： Phenolic compounds pathway design Protein engineering Coculture engineering Modular pathway optimization Dynamic regulation

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