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Ophiostomatales (Ascomycota) associated with Tomicus species in southwestern China with an emphasis on Ophiostoma canum

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Journal of Forestry Research 2020年第6期31卷 2549-2562页

作者： Yue Pan Jun Lu Peng Chen Zefen Yu Huihong Zhang Hui Ye Tao Zhao Eco-development Academy Southwest Forestry UniversityKunming 650224People’s Republic of China Laboratory of Biological Invasion and Ecosecurity Yunnan UniversityKunming 650091People’s Republic of China Yunnan Academy of Forestry and Grassland Kunming 650201People’s Republic of China Laboratory for Conservation and Utilization of Bio-resources Key Laboratory Resources of the Ministry of EducationYunnan UniversityKunming 650091People’s Republic of China Department of Science and Technology Orebro University70182 OrebroSweden

Ophiostomatalean fungi may facilitate bark beetle colonization and *** the present study of the fungal community associated with bark beetle species belonging to Tomicus in Yunnan,China,six ophiostomatalean fungi(Ophiostoma canum,***,***,Leptographium yunnanense,Leptographium sp.1 and Leptographium sp.2)were isolated from the beetles or their galleries;*** was the most common fungal *** distribution of *** was associated with stands heavily damaged by Tomicus species and a higher percentage of valid galleries of Tomicus yunnanensis and *** in Yunnan pine(Pinus yunnanensis).After inoculation of Yunnan pine with the fungus,a phloem reaction zone formed and monoterpenes accumulated in the *** results suggested that *** was pathogenic to Yunnan pine and that the wide distribution of the fungus might be benefi cial to reproduction of pine shoot beetles in Yunnan ***,because the reaction zone and monoterpene accumulation were mild,fungal damage of Yunnan pine might be limited.A more integrated study considering all the fungal species should be done to better understand the interactions among bark beetles,blue-stain fungi,and the tree hosts in the region.

关键词： Ophiostomatalean fungi Fungal isolation and identifi cation Ophiostoma canum Pinus yunnanensis pathogenicity test

Morphological and molecular identification of Neopestalotiopsis clavispora causing flower blight on Anthurium andraeanum in Thailand

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Horticultural Plant Journal 2021年第6期7卷 573-578页

作者： Wilailuck Daengsuwan Prisana Wonglom Siwaret Arikit Anurag Sunpapao Science Program in Plant Pathology Graduate SchoolPrince of Songkla UniversityHatyaiSongkhla 90110Thailand Agricultural Innovation and Management Division(Pest Management) Faculty of Natural ResourcesPrince of Songkla UniversityHatyaiSongkhla 90110Thailand Faculty of Technology and Community Development Thaksin UniversityPhatthalung CampusPhattalung 93110Thailand Rice Science Center Kasetsart University Kamphaeng Saen CampusNakhon Pathom73140Thailand Department of Agronomy Faculty of Agriculture at Kamphaeng SaenKasetsart University Kamphaeng Saen CampusNakhon Pathom 73140Thailand

Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern *** fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer(ITS),translation elongation factor 1-α(tef1-α),andβ-tubulin(tub)*** phylogenetic tree,based on the combined sequences of ITS,tef1-α,and tub,confirmed this pathogen as *** of the species was confirmed according to Koch’s postulate:*** could infect *** the best of our knowledge,this is the first report of *** as a pathogen of anthurium.

关键词： Flamingo flower Morphology Molecular properties Neopestalotiopsis clavispora pathogenicity test

Comparison of PCR, DIA and pathogenicity Assay for Detection of Xanthomonas axonopodis ***,the Causal Agent of Citrus Bacterial Canker Disease

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Agricultural Sciences in China 2004年第6期3卷 442-447页

作者： WANGZhong-kang SUNXian-yun YINYou-ping ZHOUChang-yong XIAYu-xian ResearchCenterofGeneticEngineering KeyLabforBiomechanics＆TissueEngineeringMinistryofEducation／ChongqingUniversityChongqing400044P.R.China CollegeofPlantProtection SouthwestAgriculturalUniversityChongqing400716P.R.China

Polymerase chain reaction (PCR) approach based on newly designed primers, JYF5/JYR5, wasapplied for specific detection of Xanthomonas axonopodis ***(Xac). The efficiencyand reliability of PCR method were compared with dot immunobinding assay (DIA) andclassical pathogenicity test techniques for detecting suspensions of pure cells of Xacand soaking sap of citrus tissues. Detection sensitivity of PCR was about 4.5 cells or1.56 pg target DNA per reaction which was higher than that of DIA (ca. 450 cells per dot).These three techniques (PCR assay, DIA and Pathogenecity test) could always detect Xacfrom symptomatic citrus samples. Different performances were obtained from citrusmaterials without symptoms, and the positive detection frequency was PCR, DIA andpathogenicity test.

关键词： Xanthomonas axonopodis pv.citri PCR DIA pathogenicity test

Isolation and Identification of a High-pathogenicity Strepto- coccus suis Serotype 7 Strain

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Agricultural Biotechnology 2017年第6期6卷 35-37,41页

作者： Shuguang LI Feng LI Likun CHENG Guotao LIU Na ZHANG Lifang YANG Zhiqiang SHEN Shandong Province Research and Development and Popularization Center of Propolis Vaccine for Animals and Poultry Binzhou 256600 China Binzhou Animal Science & Veterinary Medicine Academy Binzhou 256600 China Binzhou Animal Science & Veterinary Medicine Bureau Binzhou 256600 China

In order to diagnose the diseased pigs in a certain large pig farm in Binzhou City, Shandong Province, the dead piglets with joint swelling were subjected to necroscopy, and the pathogenic bacterium was isolated and identified. One Gram-positive Streptococcus was isolated. The strain was subjected to characteristic culture, microscopic examination and molecular biological identification, and resistance detection, animal regression experiment and mouse pathogenicity test were carried out. The results showed that the isolate was identified to be Streptococcus suis serotype 7, which was resistant to multiple drugs; and the pathogenicity test showed that the strain had high pathogenicity to pigs, resulting in neurosis on partial pigs, and the strain had no pathogenicity to Kunming and BALB/c mice but certain pathogenicity to CD1 mice.

关键词： Streptococcus suis serotype 7 Isolation and identification Animal regression test pathogenicity test Molecular biological identification

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山东省葡萄白腐病病原菌鉴定

植物病理学报 2023年第4期53卷 729-733页

作者：袁丽芳尹向田蒋锡龙李廷刚魏彦锋山东省农业科学院葡萄研究院济南250100

葡萄白腐病是世界范围内葡萄种植区的重要病害,每年可导致葡萄减产20%~30%[1-2],该病的病原菌有Coniella diplodiella (Speg.) Petrak & Sydow、C. fragariae (Oudem) B. Sutton、Pilidiella castaneicola和C. vitis[3-7]。1999年,LIU等... 详细信息

关键词： grape Coniella vitis grape white rot identification pathogenicity test

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八仙花叶斑病病原的分离与鉴定

植物病理学报 2023年第4期53卷 721-724页

作者：赵娟乔广行段佳琪谭晓倩邱艳红秦文韬北京市农林科学院植物保护研究所北京100097 河北北方学院农林科技学院张家口075131 长江大学生命科学学院荆州434025 北京市农林科学院蔬菜研究所北京100097

八仙花[Hydrangea macrophylla (Thunb.)Ser.],又名绣球花、粉团花等,在城市现代公园和风景区建设中占据重要地位。八仙花在生长期常受到多种病原物危害,其中叶斑病发生普遍,病原种类复杂。已报道病原包括短小茎点霉(Phoma exigua)、交... 详细信息

八仙花[Hydrangea macrophylla (Thunb.)Ser.],又名绣球花、粉团花等,在城市现代公园和风景区建设中占据重要地位。八仙花在生长期常受到多种病原物危害,其中叶斑病发生普遍,病原种类复杂。已报道病原包括短小茎点霉(Phoma exigua)、交链格孢(Alternaria alternata)、极细枝孢(Cladosporium tenuissimum)、多主棒孢(Corynespora cassiicola)等[1-4]。2021年8月课题组在北京市丰台区发现八仙花叶斑病为害严重。

关键词： Hydrangea macrophylla Epicoccum sorghinum leaf spot pathogenicity test species identification

A pathogenic fungus Pseudopestalotiopsis theae was firstly identified from tea leaf spot disease in Kaiyang,Guizhou Province,China

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A pathogenic fungus Pseudopestalotiopsis theae was firstly i...

A pathogenic fungus Pestalotiopsis trachicarpicola was firstly identified from tea leaf spot disease in Yuqing county,Guizhou Province,China

中国植物病理学会2019年学术年会

作者： Ren Yafeng An Xiaoli Li Dongxue Bao Xingtao Jiang Shilong Wang Delu Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Forestry Guizhou University College of Agriculture Guizhou University

Tea leaf spot disease mainly damaged young leaves and shoot of tea（Camellia sinensis）and led to a huge loss of the production of tea in Kaiyang county,Guizhou *** order to identify the causal agent of this disease,we isolated and identified the *** morphologies of the colony on PDA were undulating,with regular margin and the aerial mycelia represented woolly-cottony and pure *** conidiomata containing black conidial masses were formed on the aerial *** cells were in clusters,simple filiform,hyaline and *** represented fusiform,4-septate,measured 24.99±2.10 μm（20.25-28.52 μm） x 6.00±0.70 μm（4.83-8.56 μm）,with 3-4 apical appendages,and the three median cells were brown,whereas basal cells were *** genes of translation elongation factor 1-alpha（EFl）,the internal transcribed spacer（ITS）,28 S nrDNA（LSU） and beta-tubulin（TUB） were amplified and *** parsimony phylogenetic analysis based on concatenated sequences of combined EF1（1-356）,ITS（357-969）,LSU（970-1895） and TUB（1896-2426） indicated that the strain GZKY2018 AXL4 D was identical to reference strain Pesudopestalotiopsis theae MFLUCC12-0055,and the clade was supported by 97.3% bootstrap *** fulfill the Koch’s postulate,wounded tea leaves were inoculated with actively growing mycelia plugs on *** light brown spots were formed on inoculation site after 1-2 d of *** our knowledge,this is the first report of *** causing tea leaf spot in this region,and this work will help the management of disease in the field.

关键词： Pseudopestalotiopsis theae identification pathogenicity test

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A pathogenic fungus Pestalotiopsis trachicarpicola was first...

Cladosporium cladosporioides was firstly identified from tea leaf spot disease in Yuqing,Guizhou Province,China

中国植物病理学会2019年学术年会

作者： Yin Qiaoxiu Ren Yafeng Li Dongxue Wang Xue Wu Xian Jiang Shilong Wang Yong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease caused by pathogenic fungus seriously damaged the young leaves and shoots tea,and then affects the yield and quality of tea leaves in Yuqing county,Guizhou *** was an important disease of tea plant(Camellia sinensis) in this *** symptom of the disease represents the characteristic as follow:Purple-brown or reddish-brown dots appeared on leaves in the initial stage,and then the lesions developed into round or amorphous dark *** haloed yellow lesions were around the radial *** this study,we isolated and identified some fungi from tea leaves samples with the typical symptoms,and then conducted the pathogenicity test using Koch’s *** colonies of these isolates represent whitish to pale yellow,dense aerial mycelium,circular on PDA,with fimbriate edge,and produce black fruiting bodies,with developing in concentric *** colonies display yellow on reverse *** conidiomata was globose,black,semi-immersed on *** conidiogenous cells were fusiform,hyaline,short and thin *** consist of five cells [per size(17.49-30.16) μm×(4.74-7.4) μm].Basal cell represents conic to acute,hyaline,thin-walled,verruculose,2.66 to 7.70 μ*** apical cell represents conic to subcylindrical,hyaline,verruculose,2.99 to 6.69 μ*** middle three cells represent olive color,from the top the second cell was 3.52 to7.02 μm,the third cell was 3.37 to 6.82 μm,the fourth cell was 3.11 to 7.26 μm,two to three apical appendages,length of 6.69 to 14.85 μm,and a basal appendage,length of 2.66 to 7.70 μ*** primers ITS4/ITS5 for internal transcribed spacer(ITS) region,T1/BT2 B for β-tubulin gene(TUB),and EF1-728 F/EF-2 for translation elongation factor 1-alpha(TEF-1α) was used to amplify,the sequences were deposited in *** phylogenetic tree was constructed by PAUP v 4.0 b 10 software coupled with the Maximum Parsimony method,and the isolates were identical to reference strains Pestalotiopsis trachicarpicola MFLUCC 12-0266,and the

关键词： Pestalotiopsis trachicarpicola identification pathogenicity test leaf spot

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Cladosporium cladosporioides was firstly identified from tea...