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Isolation, Identification and Drug Sensitivity Test of a pathogenic escherichia coli Strain from Minks with Diarrhea

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Agricultural Biotechnology 2018年第3期7卷 81-83页

作者： Lirong XIAO Qiaoling LI Qinghui JIA Guisheng GAO Zhaoxing ZHANG Qiumei SHI Hebei Normal University of Science and Technology/Key Laboratory of Preventive Veterinary Medicine of Hebei Province Qinhuangdao 066604 China

[Objectives] The study aimed to identify the pathogen that causes diarrhea in minks. [Methods] Liver tissues were aseptically collected from dead minks with diarrhea. By bacterial isolation and culture,morphological observation,biochemical test and pathogenicity test,the isolated strain was identified as pathogenic E. coli. [Results]The pathogen causing diarrhea in minks was confirmed as a pathogenic E. coli strain. Drug sensitivity test indicated that the isolated pathogenic E. coli strain was highly sensitive to ceftazidime,cefotaxime,enrofloxacin,florfenicol and cephradine,moderately sensitive to ampicillin,ciprofloxacin,amikacin,doxycycline,lincomycin and gentamycin,and resistant to amoxycillin,neomycin,spectinomycin,polymyxin and penicillin. [Conclusions] This study provided reference for the prevention and control of abortion in female minks in Qinhuangdao region.

关键词： Mink Diarrhea pathogenic escherichia coli Drug sensitivity test

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Development of a Multivalent Inactivated Vaccine against pathogenic escherichia coli Infection in Forest Musk Deer

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Agricultural Science & Technology 2010年第9期11卷 97-100页

作者：罗燕康纪平程建国蔡永华代晓阳李秋波王成旭杨杰四川农业大学都江堰校区资源保护系四川都江堰611830 四川养麝研究所四川都江堰611830

A multivalent inactivated escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al（OH）3 adjuvant was *** vaccine did not cause any adverse reactions in forest musk *** immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk *** serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination *** serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third *** forest musk deer showed serum antibody titers which were obtained due to *** forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody *** serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days *** maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic escherichia coli strains（serotypes O4,O26,and O139）for 137 days after birth（during the nursing period and the period when the forest musk deer were susceptible to diseases）.

关键词： Forest musk deer pathogenic escherichia coli Multivalent inactivated vaccine Al(OH)3 adjuvant Serum antibodies Micro-agglutination test

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Effect of environmental stresses on the survival and cytotoxicity of Shiga toxin-producing escherichia coli

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Food Quality and Safety 2017年第2期1卷 139-146页

作者： Brian Byongkwon Yoo Yanhong Liu Vijay Juneja Lihan Huang Cheng-An Hwang Residue Chemistry and Predictive Microbiology Research Unit and Molecular Characterization of Foodborne Pathogens Research Unit Eastern Regional Research CenterAgricultural Research ServiceUnited States Department of AgricultureWyndmoorPennsylvaniaUSA Present address:Clinical and Environmental Microbiology Branch Division of Healthcare Quality and PromotionNational Center for Emerging and Zoonotic Infectious DiseasesCenters for Disease Control and Prevention1600 Clifton Rd.NEAtlantaGA 30329USA

The objective of this study was to determine the effect of environmental stresses on the cytotoxicity of Shiga toxin-producing escherichia coli (STEC). STEC O157:H7 and six non-O157 STEC strains (O26:H11, O103:H1, O104:H4, O111:NM, O121:NM, and O145:NM) were subjected to osmotic (aw 0.95-0.98), acid (pH 4-7), and chlorine (1-5 ppm) stresses. After stress treatments, bacterial populations, expression of virulence-associated genes, and Vero-cytotoxicity were determined. Among the strains, O145:NM survived at aw 0.97 longer than other serotypes, while O111:NM was significantly more sensitive to osmotic stress. At pH 4, O103:H1 was more resistant to the stress, while O26:H11 and O111:NM had significantly less growth. For 2 ppm chlorine stress, O26:H11, O103:H1, and O145:NM had higher populations (>3 log) than other strains. Stressed strains showed a significant increase in relative gene expression levels of stx1, stx2, and eae in O103:H1, O104:H4, and O145:NM than non-stressed control. Additionally, significantly higher Vero-cytotoxicity, as indicated by lactate dehydrogenase assay, of stressed O26:H11, O103:H1, O104:H4, and O145:NM was observed. The results suggest that the growth and cytotoxicity of selected pathogenic E. coli may be enhanced after being exposed to environmental stresses.

关键词： pathogenic escherichia coli stress growth virulence genes Vero-cytotoxicity.

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Diarrheic escherichia coli: A Predominant Etiological Agent of Gastroenteritis, a Case Study in Douala, Cameroon

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Journal of Biosciences and Medicines 2023年第6期11卷 1-17页

作者： Amandine Plidikoua Olivier Ziem Justice T. Ngom Afsa Mamboune Balbine Adande Clémence Francioli Koro Koro Rosalie Annie Ngono Ngane Department of Biochemistry Faculty of Sciences University of Douala Douala Cameroon Hygiene and Environment Department-Microbiology Section Pasteur Center of Cameroon Yaoundé Cameroon

Context: Gastroenteritis remains an infectious disease with high morbidity and mortality particularly in low incomes countries, where the capacity to search all etiological agents, especially pathogenic escherichia coli, is very limited. We investigated the contribution of pathogenic escherichia coli and their antibiotic resistance profiles in cases of gastroenteritis. Methods: A cross-sectional study was carried out on human stool samples from October 2021 to June 2022 at Laquintinie Hospital. Samples were received from patients of all age groups and screened for bacteriological and parasitological identification by microscopy, bacterial culture, biochemical identification, and antimicrobial susceptibility tests. Results: A total of 296 patients with gastroenteritis complaints, were enrolled in the study with ages ranging from 5 months to 90 years old (Median = 35.5;SD = 20.8). Among the samples analyzed, 1.7% (n = 5/296) were positive for parasites and 27% (n = 80/296) were positive for bacterial pathogens. Parasites were found in mono parasitism, mainly Entamoeba histolytica (60%;n = 3/5), followed by Trichomonas intestinalis (20%;n = 1/5), and Giardia intestinalis (20%;n = 1/5). Three species of bacterial pathogens were identified with no co-infection: diarrheic escherichia coli (DEC), Salmonella sp, and Shigella sp with respective proportions of 90% (n = 72/80), 6.3% (n = 5/80), and 3.7% (n = 3/80). For antibiotic resistance profiles (ARPs) of the 72 isolates of DEC, high levels of resistance were observed globally with amoxicillin (93.1%;n = 67/72), followed by ciprofloxacin (75%;n = 54/72), and to trimethoprim + sulfamethazole (73.6%;n = 53/72). In contrast, DEC showed low resistance rates with nitrofurans (6.9%;n = 5/72) and imipenem (2.8%;n = 2/72). The strains had 56 distinct ARPs, of which 88.9% (n = 64/72) were MDR. Salmonella sp and Shigella sp showed high levels of resistance to amoxicillin and trimethoprim + sulfamethazole. Conclusion: These results e

关键词： Gastroenteritis Enteropathogens pathogenic escherichia coli Antibiotic Resistance Multidrug-Resistance

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