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Cloning,Sequencing of F and HN Genes and Molecular Characteristics of Goose paramyxovirus BZ02 Isolate

Animal Husbandry and Feed Science 2009年第6期1卷 24-26,41页

作者： XIAO Yue-qiang YANG Chun-fu GUAN YU WU Xin-ming LIU Ji-san SHEN Zhi-qiang Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600 China Shandong Lvdu Bio-science&Technology Co. Ltd Binzhou 256600 China

[ Objective] The aim of this study was to clone and sequence F and HN genes of the isolated goose paramyxovirus and thus further investigate on its pathogenesis and evolutionary origins. [ Method] According to the published F and HN gene sequences of Avian paramyxovirus Typelat home and abroad, two pairs of primers （ FF, FR; HNF, HNR） were designed by DNAstar software, and Fand HNgenes were also amplified by PCR. The PCR products were recovered and ligated to T vector, while the positive clones were identified by ampicillin plate screening and PCR. After bacteria shaking, the plasmid was extracted and sent to Shanghai Sangon for sequencing. The sequences were compared with the sequences in GenBank, and the phylogenetic tree was drawn by DNAstar software. Meanwhile, the phylogenetic analysis of amino acid residues was also studied. [Result] The ORF of Fgene was 1 662 bp encoding 553 amino acids, and its cleavage site was 112R-R-Q-K-R-F117, which was consistent with the characteristics of virulent strains. 101-bit and 121-bit amino acid residues were K （Lys） and D （Asp）. The ORF of HN gene was 1 716 bp encoding 571 amino acids. The homology of HN sequence in 29 strains of goose paramyxovirus to YG97 was the highest, accounting for 99.6%. Analysis of glycosylation sites revealed that glycosylation site of BZ02 strain at 538 -540aa disappeared. The phylogenetic tree drawn by HN genes was highly consistent with that drawn by Fgenes. Compared with the homology of F and HN nucleotide sequence of the published typelgoose paramyxovirus in China, BZ02, JG97, HG97 and YG97 were divided into the same sub-group, and the nucleotide and amino acid sequences homologies were highest between BZ02 and YG97 strain. E Condusionl BZ02, JG97, HG97 and YG97 have the same evolutionary origin or all originate from YG97.

关键词： paramyxovirus BZ02 strain Evolutionary origin

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Discovery and characterization of novel paramyxoviruses from bat samples in China

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Virologica Sinica 2023年第2期38卷 198-207页

作者： Haoxiang Su Yuyang Wang Yelin Han Qi Jin Fan Yang Zhiqiang Wu NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100730China Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100730China

Many paramyxoviruses are responsible for a variety of mild to severe human and animal *** on the novel discoveries over the past several decades,the family Paramyxoviridae infecting various hosts across the world includes 4 subfamilies,17 classified genera and 78 species ***,no systematic surveys of bat paramyxoviruses are available from the Chinese *** this study,13,064 samples from 54 bat species were collected and a comprehensive paramyxovirus survey was *** obtained 94 new genome sequences distributed across paramyxoviruses from 22 bat species in seven *** phylodynamic and phylogenetic analyses showed that there were four different lineages in the Jeilongvirus *** on available data,results of host and region switches showed that the bat colony was partial to interior,whereas the rodent colony was exported,and the felines and hedgehogs were most likely the intermediate hosts from Scotophilus *** than *** on the evolutionary trend,genus Jeilongvirus may have originated from Mus *** Australia,then transmitted to bats and rodents in Africa,Asia and Europe,and finally to bats and rodents in America.

关键词： paramyxovirus Jeilongvirus Bat Host and region switches China

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Characterization of Pigeon paramyxoviruses (Newcastle disease virus) Isolated in Kazakhstan in 2005

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Virologica Sinica 2012年第2期27卷 93-99页

作者： Andrey Bogoyavlenskiy Vladimir Berezin Alexey Prilipov Eugeniy Usachev Ilya Korotetskiy Irina Zaitceva Aydyn Kydyrmanov Marat Sayatov Institute of Microbiology & Virology Almaty 050010 Kazakhstan D.I. Ivanovsky Institute of Virology Russian Academy of medical sciences Moscow 123098 Russia

Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47-422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.

关键词： Newcastle disease virus paramyxovirus Phylogenetic characterization Pigeon

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The Mechanism of Henipavirus Fusion:Examining the Relationships between the Attachment and Fusion Glycoproteins

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Virologica Sinica 2009年第2期24卷 110-120页

作者： Andrew C. Hickey Christopher C. Broder Department of Microbiology and Immunology Uniformed Services University of the Health Sciences

The henipaviruses,represented by Nipah virus and Hendra virus,are emerging zoonotic viral pathogens responsible for repeated outbreaks associated with high morbidity and mortality in Australia,Southeast Asia,India and Bangladesh. These viruses enter host cells via a class I viral fusion mechanism mediated by their attachment and fusion envelope glycoproteins;efficient membrane fusion requires both these glycoproteins in conjunction with specific virus receptors present on susceptible host cells. The henipavirus attachment glycoprotein interacts with a cellular B class ephrin protein receptor triggering conformational alterations leading to the activation of the viral fusion(F) glycoprotein. The analysis of monoclonal antibody(mAb) reactivity with G has revealed measurable alterations in the antigenic structure of the glycoprotein following its binding interaction with receptor. These observations only appear to occur with full-length native G glycoprotein,which is a tetrameric oligomer,and not with soluble forms of G(sG) ,which are disulfide-linked dimers. Single amino acid mutations in a heptad repeat-like structure within the stalk domain of G can disrupt its association with F and subsequent membrane fusion promotion activity. Notably,these mutants of G also appear to confer a post-receptor bound conformation implicating the stalk domain as an important element in the G glycoprotein's structure and functional relationship with F. Together,these observations suggest fusion is dependent on a specific interaction between the F and G glycoproteins of the henipaviruses. Further,receptor binding induces measurable changes in the G glycoprotein that appear to be greatest in respect to the interactions between the pairs of dimers comprising its native tetrameric structure. These receptor-induced conformational changes may be associated with the G glycoprotein's promotion of the fusion activity of F.

关键词： Hendra virus Nipah virus Henipavirus paramyxovirus Viral entry

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Immune responses elicited by ssRNA(-) oncolytic viruses in the host and in the tumor microenvironment

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Journal of Cancer Metastasis and Treatment 2023年第1期9卷 285-306页

作者： Yonina Bykov Gloria Dawodu Aryana Javaheri Adolfo Garcia-Sastre Sara Cuadrado-Castano Department of Microbiology Icahn School of Medicine at Mount SinaiNew YorkNY 10029USA Department of Medicine Icahn School of Medicine at Mount SinaiNew YorkNY 10029USA Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount SinaiNew YorkNY 10029USA The Tisch Cancer Institute Icahn School of Medicine at Mount SinaiNew YorkNY 10029USA Department of Pathology Molecular and Cell-Based MedicineIcahn School of Medicine at Mount SinaiNew YorkNY 10029USA

Oncolytic viruses(OVs)are at the forefront of biologicals for cancer *** represent a diverse landscape of naturally occurring viral strains and genetically modified viruses that,either as single agents or as part of combination therapies,are being evaluated in preclinical and clinical *** the field gains momentum,the research on OVs has been shifting efforts to expand our understanding of the complex interplay between the virus,the tumor and the immune system,with the aim of rationally designing more efficient therapeutic ***,the potential of an OV platform is no longer defined exclusively by the targeted replication and cancer cell killing capacities of the virus,but by its contribution as an immunostimulator,triggering the transformation of the immunosuppressive tumor microenvironment(TME)into a place where innate and adaptive immunity players can efficiently engage and lead the development of tumor-specific long-term memory *** we review the immune mechanisms and host responses induced by ssRNA(-)(negative-sense single-stranded RNA)viruses as OV *** focus on two ssRNA(-)OV candidates:Newcastle disease virus(NDV),an avian paramyxovirus with one of the longest histories of utilization as an OV,and influenza A(IAV)virus,a well-characterized human pathogen with extraordinary immunostimulatory capacities that is steadily advancing as an OV candidate through the development of recombinant IAV attenuated platforms.

关键词： Oncolytic virus NDV IAV virotherapy paramyxovirus orthomyxovirus ssRNA(-) immunotherapy cancer vaccine ICD in situ vaccination tumor microenvironment

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