检索结果-南通市图书馆

Claudin-4 is required for AMPK-modulated paraceliular permeability in submandibular gland ceils

Journal of Molecular Cell Biology 2014年第6期8卷 486-497页

作者： Ruo-Lan Xiang Mei Mei Xin Cong Jing Li Yan Zhang Chong Ding Li-Ling Wu Guang-Yan Yu Center for Salivary Gland Diseases of Peking University School and Hospital of Stomatology Department of Physiology and PathophysiologySchool of Basic Medical Sciences Peking University Health Science Center and Key Laboratory of Molecular Cardiovascular Sciences Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology Beijing 100081 China Central Laboratory Peking University School and Hospital of Stomatology Belling 100081 China

Tight junction plays an important rote in mediating paraceUular permeability in epithelia. We previously found that activation of AMP- activated protein kinase （AMPK） increased saliva secretion by modulating paraceUular permeability in submandibular glands. However, the molecular mechanisms underlying AMPK-modulated paraceUular permeability are unknown. In this study, we found that AICAR, an AMPK agonist, increased saliva secretion in the isolated rat submandibular glands, decreased transepithelial electrical resistance （TER）, and increased 4 kDa FITC-dextran flux in cultured SMG-C6 cells. AICAR also induced redistribution of tight junction protein claudin-4, but not claudin-1, claudin-3, occtudin, or ZO-1, from the cytoplasm to the membrane. Moreover, knockdown of claudin-4 by shRNA suppressed while claudin-4 re-expression restored the TER and 4 kDa FITC-dextran flux responses to AICAR. Additionally, AICAR increased ERK1/2 phosphorylation, and inhibition of ERK1/2 by U0126, an ERK1/2 kinase inhibitor, or by siRNA decreased AICAR-induced TER responses. AICAR induced the serine S199 phosphorylation of claudin-4 and enhanced the inter- action of claudin-4 and occludin. Furthermore, pretreatment with U0126 significantly suppressed AMPK-modulated phosphorytation, redistribution, and interaction with occludin of claudin-4. Taken together, these results indicated that claudin-4 played a crucial role in AMPK-rnodutated paraceUular permeability and ERK1/2 was required in AMPK-modulated tight junction barrier function in subman- dibular gland.

关键词： AMP-activated protein kinase daudin-4 tight junction paracellular permeability submandibular gland

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intestinal microbiota: a potential target for the treatment of postmenopausal osteoporosis

引用

Bone Research 2017年第3期5卷 177-194页

作者： Xin Xu Xiaoyue Jia Longyi Mo Chengcheng Liu Liwei Zheng Quan Yuan Xuedong Zhou State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Department of Cariology and Endodontics West China Hospital of Stomatology Sichuan University Department of Periodontics West China Hospital of Stomatology Sichuan University Department of Pediatric Dentistry West China Hospital of Stomatology Sichuan University Department of Dental Implantology West China Hospital of Stomatology Sichuan University

Postmenopausal osteoporosis （PMO） is a prevalent metabolic bone disease characterized by bone loss and structural destruction, which increases the risk of fracture in postmenopausal women. Owing to the high morbidity and serious complications of PMO, many efforts have been devoted to its prophylaxis and treatment. The intestinal microbiota is the complex community of microorganisms colonizing the gastrointestinal tract. Probiotics, which are dietary or medical supplements consisting of beneficial intestinal bacteria, work in concert with endogenous intestinal microorganisms to maintain host health. Recent studies have revealed that bone loss in PMO is closely related to host immunity, which is influenced by the intestinal microbiota. The curative effects of probiotics on metabolic bone diseases have also been demonstrated. The effects of the intestinal microbiota on bone metabolism suggest a promising target for PMO management. This review seeks to summarize the critical effects of the intestinal microbiota and probiotics on PMO, with a focus on the molecular mechanisms underlying the pathogenic relationship between bacteria and host, and to define the possible treatment options.

关键词： CHAIN FATTY-ACIDS BONE-MINERAL DENSITY TUMOR-NECROSIS-FACTOR INCREASE CALCIUM-ABSORPTION ESTROGEN-RECEPTOR-ALPHA REGULATORY T-CELLS LONG-TERM CONSUMPTION GUT MICROBIOTA VITAMIN-D paracellular permeability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regulation of intestinal permeability: The role of proteases

引用

World Journal of Gastroenterology 2017年第12期23卷 2106-2123页

作者： Hanne Van Spaendonk Hannah Ceuleers Leonie Witters Eveline Patteet Jurgen Joossens Koen Augustyns Anne-Marie Lambeir Ingrid De Meester Joris G De Man Benedicte Y De Winter Laboratory of Experimental Medicine and Pediatrics Division of GastroenterologyUniversity of Antwerp Laboratory of Medicinal Chemistry and Antwerp Drug Discovery Network University of Antwerp Laboratory of Medical Biochemistry University of Antwerp

The gastrointestinal barrier is-with approximately 400 m^2-the human body's largest surface separating the external environment from the internal milieu. This barrier serves a dual function: permitting the absorption of nutrients, water and electrolytes on the one hand, while limiting host contact with noxious luminal antigens on the other hand. To maintain this selective barrier, junction protein complexes seal the intercellular space between adjacent epithelial cells and regulate the paracellular transport. Increased intestinal permeability is associated with and suggested as a player in the pathophysiology of various gastrointestinal and extraintestinal diseases such as inflammatory bowel disease, celiac disease and type 1 diabetes. The gastrointestinal tract is exposed to high levels of endogenous and exogenous proteases, both in the lumen and in the mucosa. There is increasing evidence to suggest that a dysregulation of the protease/antiprotease balance in the gut contributes to epithelial damage and increased permeability. Excessive proteolysis leads to direct cleavage of intercellular junction proteins, or to opening of the junction proteins via activation of protease activated receptors. In addition, proteases regulate the activity and availability of cytokines and growth factors, which are also known modulators of intestinal permeability. This review aims at outlining the mechanisms by which proteases alter the intestinal permeability. More knowledge on the role of proteases in mucosal homeostasis and gastrointestinal barrier function will definitely contribute to the identification of new therapeutic targets for permeability-related diseases.

关键词： Intestinal permeability Intestinal barrier Tight junction paracellular permeability Proteases Proteinase-activated receptor Protease inhibitor Antiproteases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Higher doses of ascorbic acid may have the potential to promote nutrient delivery via intestinal paracellular absorption

引用

World Journal of Gastroenterology 2021年第40期27卷 6750-6756页

作者： Ivana Roosevelt Sequeira Human Nutrition Unit School of Biological ScienceUniversity of AucklandAuckland 1024New Zealand

The significance of plasma ascorbic acid(AA)is underscored by its enzymatic and antioxidant properties as well as involvement in many aspects of health including the synthesis of biomolecules during acute illness,trauma and chronic health *** intake supports maintenance of optimal levels with supplementation at higher doses more likely *** increased intestinal paracellular permeability following high dose AA may be utilised to enhance delivery of other micronutrients across the intestinal *** potential mechanism following dietary intake however needs further study but may provide an avenue to increase small intestinal nutrient co transport and absorption,including in acute and chronic illness.

关键词： Aspirin Ascorbic acid paracellular permeability Antioxidant Lactulose mannitol test

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Claudin-1 Leads to Strong Formation of Tight Junction in Cultured Mouse Lung Microvascular Endothelial Cells

引用

Pharmacology & Pharmacy 2016年第3期7卷 133-139页

作者： Yukari Ueda Yasuhumi Shinmyouzu Hikaru Nakayama Tadatoshi Tanino Eiko Sakurai Eiichi Sakurai Department of Pharmaceutics Faculty of Pharmaceutical Sciences Tokushima Bunri University Tokushima Japan Faculty of Pharmacy Iwaki Meisei University Fukushima Japan

We aimed to examine paracellular barrier function in cultured mouse lung microvascular endothelial cells (LMECs). The transcellular resistance of LMEC monolayers yielded an electrical resistance of approximately 19 Ω × cm2 at days 6 - 7 in culture when the cells reached confluence, and paracellular permeable clearance of sodium fluorescein was the lowest on day 6 in culture, suggesting the formation of tight junctions (TJs) in cultured LMECs. Moreover, the expression of TJ-associated proteins, occludin, claudin-1 and -4 and zonula occludents 1 (ZO-1) was detected in LMECs at day 6 in culture. However, mRNAs of occludin, claudin-1 and -4 and ZO-1 were already expressed on day 1 after culture, and large variations were absent in the mRNA levels of occludin, claudin-4 and ZO-1 between days 1 and 7 in culture, when the level of each mRNA on day 1 in culture was used as a basal level. However, the claudin-1 mRNA level gradually increased up to approximately 7-fold on day 7 in culture over the basal level. These results indicate that the drastic increase in the mRNA expression level of claudin-1 leads to the strong formation of TJs.

关键词： Mouse Lung Microvascular Endothelial Cells paracellular permeability Tight Junction Occludin Claudins Zonula Occludents

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：