We previously observed the elicitation of a significant delayed-type hypersensitivity response to the panton-valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al., Clinical Micro...
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We previously observed the elicitation of a significant delayed-type hypersensitivity response to the panton-valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al., Clinical Microbiology and Infection, 2009, 156: 156-164). A LukS-PV-specific cell line (LST) was screened for proliferative responses against a panel of 25 amino acid-long peptides spanning the length of LukS-PV (amino acids 29-312). This analysis demonstrated that stimulation of LST with LukS-PV resulted in significant proliferative responses and adoptive transfer of LST into na?ve mice conferred a LukS-PV-specific DTH response following challenge. Challenge of mice adoptively transferred with LST with peptides 7 (149-173), 8 (169-193) and 14 (289-312) also elicited a measurable DTH response suggesting that these peptides contained T cell epitopes.
Objective:To identify the pathogenic gene of panton-valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn ***:A total of 104 ...
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Objective:To identify the pathogenic gene of panton-valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn ***:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July *** isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant *** the presence of the panton-valentine leukocidin gene was tested by ***:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the panton-valentine leukocidin gene,and only 20%of the samples had panton-valentine leukocidin *** and female patients showed no significant difference in the positivity rate of panton-valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the panton-valentine leukocidin ***:In recent years,increased resistance has been a serious *** resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.
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