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The Synthesis and Characterization of β-cyclodextrin Derivated pancreatin Used as Chiral Selector in Capillary Electrochromatography

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Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 27-28页

作者：戴荣继 School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China

The preparation of capillary electrochromatography column for chiral separation with β-cyclodextrin derivatized pancreatin was imvestigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether （EGDE） in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sot-gel technology using triethoxylmethylsiloxane and （ 3-aminopropyl ） trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glataraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baseline by β-cyclodetrin derivated pancreatin in capillary electrochromatography.

关键词： capillary electrochromatography pancreatin chiral separation β-cyclodextrin

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The effect of enzymes on the in vitro degradation behavior of Mg alloy wires in simulated gastric fluid and intestinal fluid

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Bioactive Materials 2022年第1期7卷 217-226页

作者： Yue Zhang Jian Cao Xianli Wang Huan Liu Yi Shao Chenglin Chu Feng Xue Jing Bai School of Materials Science and Engineering Southeast UniversityJiangningNanjing 211189JiangsuChina Jiangsu Key Laboratory for Advanced Metallic Materials JiangningNanjing 211189JiangsuChina Institute of Biomedical Devices(Suzhou) Southeast UniversitySuzhou 215163JiangsuChina d Peking University People’s Hospital Xi ChengBeijing 100044China College of Mechanics and Materials Hohai UniversityNanjing211100China

With an upsurge of biodegradable metal implants,the research and application of Mg alloys in the gastrointestinal environment of the digestive tract have been of great *** enzymes,mainly pepsin in the stomach and pancreatin in the small intestine,are widespread in the gastrointestinal tract,but their effect on the degradation of Mg alloys has not been well *** this study,we investigated the impacts of pepsin and pancreatin on the degradation of Mg-2Zn alloy *** results showed that the pepsin and pancreatin had completely different even the opposite effects on the degradation of Mg,although they both affected the degradation product *** degradation rate of Mg wire declined with the addition of pepsin in simulated gastric fluid(SGF)but rose with the addition of pancreatin in simulated intestinal fluid(SIF).The opposite trends in degradation rate also resulted in completely different degradation morphologies in wires surface,where the pitting corrosion in SGF was inhibited because of the physical barrier effect of pepsin *** contrast,the adsorption of pancreatin affected the integrity of magnesium hydrogen phosphate film,causing a relatively uneven degraded *** results may help us to understand the role of different digestive enzymes in the degradation of magnesium and facilitate the development and clinical application of magnesium alloy implanted devices for the digestive tract.

关键词： Degradable Mg alloy Pepsin pancreatin Gastrointestinal Digestive tract

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Indigestible cowpea proteins reduced plasma cholesterol after long-term oral administration to Sprague-Dawley rats

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Food Production, Processing and Nutrition 2021年第1期3卷 203-213页

作者： Adeola M.Alashi Hongyi Wu Rotimi E.Aluko Department of Food and Human Nutritional Sciences University of ManitobaWinnipeg R3T 2N2Canada The Richardson Center for Functional Foods and Nutraceuticals University of ManitobaWinnipeg R3T 2N2Canada

Cowpea protein isolate(CPI)was subjected to various dry and wet heat pretreatments followed by sequential digestion with pepsin and pancreatin;the undigested residues were isolated as the indigestible cowpea proteins(ICPs).All the ICPs exhibited in vitro bile acid-binding capacity but ICP from the slow cooling-induced gelation had the highest yield(68%)and was used for rat feeding experiments to determine effect on plasma total cholesterol(TC).Groups consisting of 3 male and 3 female Sprague-Dawley rats each were fed hypercholesterolemic diets that contained casein only or casein that was partially substituted with ICP of CPI for 6 *** showed diet that contained 5%(w/w)ICP was more effective in preventing TC increase(1.8 mmol/L)when compared to increases of 9.34 and 4.15 mmol/L for CPI and casein only diets,respectively.

关键词： Cowpea Pepsin pancreatin Indigestible proteins Cholesterol SDS-PAGE

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A Study of the Enzymatic Hydrolysis of Fish Frames Using Model Systems

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Food and Nutrition Sciences 2011年第6期2卷 575-585页

作者： Aristotelis T. Himonides Anthony K. D. Taylor Anne J. Morris Technological Educational Institute Thessaloniki Greece

A model system was employed to study the operating conditions and primary parameters of enzymic hydrolysis of cod proteins. pancreatin, papain, and bromelain were used to hydrolyse minced cod fillets under controlled conditions and with the rate of hydrolysis being continually monitored via both the pH-stat and TNBS method. The two methods were compared and evaluated. The rate of protein solubilisation was plotted against the degree of hydrolysis (DH). Dry fish protein hydrolysate (FPH) powders having short, medium and high degrees of hydrolysis (DH of approximately 8%, 11% and 16% respectively) were produced and analysed for their molecular weight distribution, using size exclusion chromatography. Almost complete protein solubilisation (75 g soluble protein per kg hydrolysis solution) could be achieved within an hour, at 40oC, at 1% enzyme/substrate ratio (w/w) with papain and bromelain. The pH-stat was found capable of continuously following the rate of hydrolysis but only at low DH. The TNBS could be accurately used even at high DH to estimate the percentage of the peptide bonds cleaved, but required chemical analysis of withdrawn samples.

关键词： Enzymatic Hydrolysis Papain Bromelain pancreatin Fish Frames Cod Fish Protein Hydrolysates

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猪胰脏联产胰酶、弹性酶和激肽释放酶的研究

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辽宁大学学报(自然科学版) 1991年第4期 51-55页

作者：夏盛强杨淑琴陈淑英于杰沈阳市计划生育研究所辽宁大学生物系丹东市商检局

本文报导一种综合利用猪胰脏的方法。猪胰脏经溶剂提取、盐析得到胰酶(Pauoieatin),弹性酶(eleitael),再从提取酶后的废液中,DEAE-32纤维素制得胰激肽释放酶(Kallikiein)。

关键词： pancreatin Elestase Kallikrein

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