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palmitic acid reduces the methylation of the FOXO1 promoter to suppress the development of diffuse large B-cell lymphoma via modulating the miR-429/DNMT3A axis

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Chinese Journal of Natural Medicines 2024年第6期22卷 554-567页

作者： LI Weiming GAO Ming XUE Weili LI Xiaoli CHANG Yu ZHANG Kaixin WEN Chenyu ZHANG Mingzhi Department of Oncology Henan University of Traditional Chinese MedicineZhengzhou 450000China Department of Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China

Diffuse large B-cell lymphoma(DLBCL)is characterized by significant treatment *** acid(PA)has shown promising antitumor *** study aims to elucidate the molecular mechanisms by which PA influences DLBCL *** quantified the expression levels of microRNAs(miRNAs),Forkhead box protein O1(FOXO1),and DNA methyltransferase 3A(DNMT3A)in both untreated and PA-treated DLBCL tumors and cell *** were made of cell viability,apoptosis,and autophagy-related protein expression following PA *** analyses among miR-429,DNMT3A,and FOXO1 were conducted using luciferase reporter assays and methylation-specific(MSP)Polymerase chain reaction(PCR).After transfecting the miR-429 inhibitor,negative control(NC)inhibitor,shRNA against DNMT3A(sh-DNMT3A),shRNA negative control(sh-NC),over-expression vector for DNMT3A(oe-DNMT3A),or overexpression negative control(oe-NC),we evaluated the effects of miR-429 and DNMT3A on cell viability,mortality,and autophagy-related protein expression in PA-treated DLBCL cell *** efficacy of PA was also tested in vivo using DLBCL tumor-bearing mouse ***-429 and FOXO1 expression levels were downregulated,whereas DNMT3A was upregulated in DLBCL compared to the control *** treatment was associated with enhanced autophagy,mediated by the upregulation of miR-429 and downregulation of *** luciferase reporter assay and MSP confirmed that miR-429 directly inhibits DNMT3A,thereby reducing FOXO1 *** experiments demonstrated that PA promotes autophagy and inhibits DLBCL progression by upregulating miR-429 and modulating the DNMT3A/FOXO1 *** vivo PA signific-antly reduced the growth of xenografted tumors through its regulatory impact on the miR-429/DNMT3A/FOXO1 *** acid may modulate autophagy and inhibit DLBCL progression by targeting the miR-429/DNMT3A/FOXO1 signaling pathway,suggesting a novel therapeutic target for DLBCL management.

关键词： palmitic acid Diffuse large B-cell lymphoma MiR-429 DNMT3A FOXO1 Autophagy

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Effects of Berberine and Cinnamic acid on palmitic acid-Induced Intracellular Triglyceride Accumulation in NIT-1 Pancreatic β Cells

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Chinese Journal of Integrative Medicine 2016年第7期22卷 496-502页

作者：赵莉姜淑君陆付耳徐丽君邹欣王开富董慧 Institute of Integrated Traditional Chinese and Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Nephrology Wuhan Integrated Traditional Chinese Medicine and Western Medicine Hospital Tongji Medical College Huazhong University of Science and Technology

Objective： To investigate the effects of berberine （BBR） and cinnamic acid （CA）, the main active components in Jiaotai Pill （交泰丸, JTP）, on palmitic acid （PA）-induced intracellular tdglyceride （TG） accumulation in NIT-1 pancreatic 13 cells. Methods： Cells were incubated in culture medium containing PA （0.25 mmol/L） for 24 h. Then treatments with BBR （10 μmol/L）, CA （100 μmol/L） and the combination of BBR and CA （BBR＋CA） were performed respectively. Intracellular lipid accumulation was assessed by Oil Red O staining and TG content was measured by colorimetric assay. The expression of adenosine monophosphate-activated protein kinase （AMPK） protein and its downstream lipogenic and fatty acid oxidation genes, including fatty acid synthase （FAS）, acetyl-coA carboxylase （ACC）, phosphorylation acetyl-coA carboxylase （pACC）, carnitine acyl transferase 1 （CPT-1） and sterol regulating element binding protein lc （SREBP-lc） were determined by Western blot or real time polymerase chain reaction. Results： PA induced an obvious lipid accumulation and a significant increase in intracellular TG content in NIT-1 cells. PA also induced a remarkable decrease in AMPK protein expression and its downstream targets such as pACC and CPT-I. Meanwhile, AMPK downstream lipogenic genes including SREBP-lc mRNA, FAS and ACC protein expressions were increased. Treatments with BBR and BBR＋CA, superior to CA, significantly reversed the above genes changes in NIT-1 pancreatic 13 cells. However, the synergistic effect of BBR and CA on intracellular TG content was not observed in the present study. Conclusion： It can be concluded that in vitro, BBR and BBR＋CA could inhibit PA-induced lipid accumulation by decreasing lipoqenesis and *** lipid oxidation in NIT-1 pancreatic B cells.

关键词： berberine cinnamic acid palmitic acid NIT-1 cells lipid Chinese medicine Jiaotai Pill

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Ni nanoparticles encapsulated within H-type ZSM-5 crystals for upgrading palmitic acid to diesel-like fuels

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Chinese Chemical Letters 2022年第2期33卷 803-806页

作者： Yanchun Shi Chen Gao Enhui Xing Jimei Zhang Feng Duan He Zhao Yongbing Xie CAS Key Laboratory of Green Process and Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China State Key Laboratory of Catalytic Materials and Reaction Engineering Research Institute of Petroleum ProcessingSinopecBeijing 100083China

Meso-Ni@HZSM-5 bi-functional catalysts were successfully post-encapsulated with about 3-7 nm Ni nanoparticles within HZSM-5 crystals,which exhibited significantly efficient conversion activity(67.4 g[palmitic acid]g[Ni]^(−1)h^(−1))of palmitic acid and 100%selectivity of hydrocarbons with the outstanding stability during recycling application,compared to the impregnated Ni/HZSM-5 catalyst(14.0 g[palmitic acid]g[Ni]^(−1)h^(−1)).

关键词： Post-encapsulation Ni@HZSM-5 Ni nanoparticles palmitic acid Hydrodeoxygenation Hydrocarbons

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Nerve growth factor protects against palmitic acid- induced injury in retinal ganglion cells

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Neural Regeneration Research 2016年第11期11卷 1851-1856页

作者： Pan-shi Yan Shu Tang Hai- feng Zhang Yuan-yuan Guo Zhi-wen Zeng Qiang Wen Department of Ophthalmology First Affiliated Hospital of Zhengzhou University Zhengzhou Henan Province China Department of Pharmacy First Affiliated Hospital of Zhengzhou University Zhengzhou Henan Province China Department of Biochemistry and Molecular Biology School of Basic Medicine Zhengzhou University Zhengzhou Henan Province China Shenzhen Mental Health Center and Shenzhen Key Lab for Psychological Healthcare Shenzhen Guangdong Province China Department of Clinical Pharmacology School of Basic Medicine Zhengzhou University Zhengzhou Henan Province China

Accumulating evidence supports an important role for nerve growth factor （NGF） in diabetic retinopathy. We hypothesized that NGF has a protective effect on rat retinal ganglion RGC-5 cells injured by palmitic acid （PA）, a metabolic factor implicated in the development of dia- betes and its complications. Our results show that PA exposure caused apoptosis of RGC-5 cells, while NGF protected against PA insult in a concentration-dependent manner. Additionally, NGF significantly attenuated the levels of reactive oxygen species （ROS） and malondialde- hyde （MDA） in RGC-5 cells. Pathway inhibitor tests showed that the protective effect of NGF was completely reversed by LY294002 （PI3K inhibitor）, Akt VIII inhibitor, and PD98059 （ERK1/2 inhibitor）. Western blot analysis revealed that NGF induced the phosphorylation of Akt/FoxO1 and ERK1/2 and reversed the PA-evoked reduction in the levels of these proteins. These results indicate that NGF protects RGC-5 cells against PA-induced injury through anti-oxidation and inhibition of apoptosis by modulation of the PI3K/Akt and ERK1/2 signaling pathways.

关键词： nerve regeneration RGC-5 cells palmitic acid nerve growth factor apoptosis PI3K Akt FoxOl ERK1/2 neural regeneration

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Functional lipidomics: palmitic acid impairs hepatocellular carcinoma development by modulating membrane fluidity and glucose metabolism

Functional lipidomics: palmitic acid impairs hepatocellular ...

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中国化学会第十三届全国分析化学年会

作者： Ling Lin Huali Shen Pengyuan Yang Institutes of Biomedical Sciences of Shanghai Medical School Fudan University Departments of Chemistry Fudan University

Lipids are essential cellular components and energy sources of living organisms, altered lipid composition is increasingly recognized as a signature of cancer. We performed the lipidomic analysis in a series of hepatocellular carcinoma（HCC） cells and identified over 1700 intact lipids originating from 3 major lipid categories. Comparative lipidomic screening revealed 93 significantly changed lipids and decreased palmitic acyl（C16:0）-containing glycerophospholipids（GPs） were positively associated with metastatic abilities of HCC cells. Furthermore, both in vitro and in vivo experiments designated that C16:0 incubation specifically reduced malignant cell proliferation, impaired cell invasiveness, and suppressed tumor growth in mouse xenograft models. Biochemical experiments demonstrated that C16:0 treatment decreased cell membrane fluidity and limited glucose metabolism. Phosphoproteomics approach further revealed such C16:0 incubation attenuated phosphorylation levels of m TOR and Stat3 pathway proteins. These data collectively provide the essential theoretical and experimental basis for diagnosing and treating HCCs by targeting the altered lipid metabolism.

关键词： lipidomics hepatocellular carcinoma metastasis palmitic acid glucose homeostasis

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Determination for the Binary System of Stearic acid and palmitic acid by Differential Scanning Calorimetry

Determination for the Binary System of Stearic Acid and Palm...

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第十六届全国化学热力学和热分析学术会议

作者： Yu-Ting Li~1,Ya-Fei Guo~1,Shi-Qiang Wang~1 and Tian-Long Deng~(1,2) 1 Tianjin Key Laboratory of Marine Resources and Chemistry,College of Marine Science and Engineering, Tianjin University of Science and Technology Tianjin,300457,P.R.China 2 ACS Key Laboratory of Salt Lake Resources and Chemistry,Qinghai Institute of Salt Lakes,Chinese Academy of Sciences,Xining,810008,P.R.China

＜正＞As a powerful thermal analysis method,differential scanning calorimetry（DSC） has been widely used for the determination of varieties thermodynamics and kinetics parameters,such as specific heat, reaction heat,phase diagram, crystallization rate and so *** to the advantages of instrument durability and affordable price at vary wide temperature ranges,many studies focused on to draw the multi-component phase diagram using DSC.

关键词： Differential scanning calorimetry(DSC) stearic acid palmitic acid phase diagram

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Effect of changing the proportion of C16:0 and cis-9 C18:1 in fat supplements on rumen fermentation, glucose and lipid metabolism, antioxidation capacity, and visceral fatty acid profile in finishing Angus bulls

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ANIMAL NUTRITION 2024年第3期18卷 39-48页

作者： Bai, Haixin Wang, Lubo Lambo, Modinat Tolani Li, Yang Zhang, Yonggen Northeast Agr Univ Coll Anim Sci & Technol Harbin 150030 Peoples R China

This study evaluated the effects of different proportions of palmitic (C16:0) and oleic (cis-9 C18:1) acids in fat supplements on rumen fermentation, glucose (GLU) and lipid metabolism, antioxidant function, and visceral fat fatty acid (FA) composition in Angus bulls. The design of the experiment was a randomized block design with 3 treatments of 10 animals each. A total of 30 finishing Angus bulls (21 +/- 0.5 months) with an initial body weight of 626 +/- 69 kg were blocked by weight into 10 blocks, with 3 bulls per block. The bulls in each block were randomly assigned to one of three experimental diets: (1) control diet without additional fat (CON), (2) CON + 2.5% palmitic calcium salt (PA; 90% C16:0), (3) CON + 2.5% mixed FA calcium salts (MA; 60% C16:0 + 30% cis-9 C18:1). Both fat supplements increased C18:0 and cis9 C18:1 in visceral fat (P < 0.05) and up-regulated the expression of liver FA transport protein 5 (FATP5; P < 0.001). PA increased the insulin concentration (P < 0.001) and aspartate aminotransferase activity (AST; P = 0.030) in bull's blood while reducing the GLU concentration (P = 0.009). PA increased the content of triglycerides (TG; P = 0.014) in the liver, the content of the C16:0 in visceral fat (P = 0.004), and weight gain (P = 0.032), and up-regulated the expression of liver diacylglycerol acyltransferase 2 (DGAT2; P < 0.001) and stearoyl-CoA desaturase 1 (SCD1; P < 0.05). MA increased plasma superoxide dismutase activity (SOD; P = 0.011), reduced the concentration of acetate and total volatile FA (VFA) in rumen fluid (P < 0.05), and tended to increase plasma non-esterified FA (NEFA; P = 0.069) concentrations. Generally, high C16:0 fat supplementation increased weight gain in Angus bulls and triggered the risk of fatty liver, insulin resistance, and reduced antioxidant function. These adverse effects were alleviated by partially replacing C16:0 with cis-9 C18:1.

关键词： palmitic acid Oleic acid Lipid metabolism Lipotoxicity Finishing bull SUBCUTANEOUS ADIPOSE-TISSUE OLEIC-acid INSULIN-RESISTANCE MILK-PRODUCTION DAIRY-COWS PRODUCTION RESPONSES STEARIC-acid PALM OIL BODY-FAT LIVER

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Ferroptosis inhibitors reduce celastrol toxicity and preserve its insulin sensitizing effects in insulin resistant HepG2 cells

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Journal of Integrative Medicine 2024年第3期22卷 286-294页

作者： Jia-jia Liu Xue Zhang Bang-lan Cai Man-man Qi Yong-bin Chi Bin Peng Deng-hai Zhang School of Medicine Shanghai UniversityShanghai 200444China Shanghai Health Commission Key Lab of Artificial Intelligence-Based Management of Inflammation and Chronic Diseases Shanghai Pudong Gongli HospitalSecondary Military Medical UniversityShanghai 200135China School of Basic Medicine Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

Objective:Research has shown that celastrol can effectively treat a variety of diseases,yet when passing a certain dosage threshold,celastrol becomes toxic,causing complications such as liver and kidney damage and erythrocytopenia,among *** this dichotomy in mind,it is extremely important to find ways to preserve celastrol’s efficacy while reducing or preventing its ***:In this study,insulin-resistant Hep G2(IR-Hep G2)cells were prepared using palmitic acid and used for in vitro ***-Hep G2 cells were treated with celastrol alone or in combination with Nacetylcysteine(NAC)or ferrostatin-1(Fer-1)for 12,24 or 48 h,at a range of *** counting kit-8assay,Western blotting,quantitative reverse transcription-polymerase chain reaction,glucose consumption assessment,and flow cytometry were performed to measure celastrol’s cytotoxicity and whether the cell death was linked to ***:Celastrol treatment increased lipid oxidation and decreased expression of anti-ferroptosis proteins in IR-Hep G2 *** downregulated glutathione peroxidase 4(GPX4)m *** docking models predicted that solute carrier family 7 member 11(SLC7A11)and GPX4 were covalently bound by ***,we found for the first time that the application of ferroptosis inhibitors(especially NAC)was able to reduce celastrol’s toxicity while preserving its ability to improve insulin sensitivity in IR-Hep G2 ***:One potential mechanism of celastrol’s cytotoxicity is the induction of ferroptosis,which can be alleviated by treatment with ferroptosis *** findings provide a new strategy to block celastrol’s toxicity while preserving its therapeutic effects.

关键词： Celastrol Insulin resistance Ferroptosis N-Acetylcysteine palmitic acid Molecular docking

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Structural and electronic effects boosting Ni-doped Mo_(2)C catalyst toward high-efficiency C-O/C-C bonds cleavage

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Journal of Energy Chemistry 2022年第12期75卷 109-116,I0004页

作者： Xiangze Du Jinjia Liu Dan Li Hui Xin Xiaomei Lei Rui Zhang Linyuan Zhou Huiru Yang Yan Zeng Hualong Zhang Wentao Zheng Xiaodong Wen Changwei Hu Key Laboratory of Green Chemistry and Technology College of ChemistrySichuan UniversityChengdu 610064SichuanChina State Key Laboratory of Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001ShanxiChina National Energy Center for Coal to Liquids Synfuels China Co.LtdBeijing 101400China Department State Key Laboratory of Catalysis iChEMDalian Institute of Chemical PhysicsChinese Academy of ScienceDalian 116023LiaoningChina Department of Chemistry University of HelsinkiA.I.Virtasen Aukio 1P.O.Box 5500014Finland

The selective cleavage of C-O and C-C is facing a challenge in the field of *** the present work,we studied the influence of doped Ni on the structure and electronic properties,as well as the selective C-O/C-C bond cleavages in the hydrodeoxygenation of palmitic acid over Ni-Mo_(2)C *** catalytic activity on Ni doped Mo_(2)C with TOF of 6.9×10^(3)h^(-1)is much superior to intrinsic Mo_(2)C catalyst,which is also higher than most noble metal ***,the doped Ni raises the active particle dispersion and the coordination numbers of Mo species(Mo-C and Mo-O),improves the graphitization degree to promote the electron transfer,and increases the amount of Lewis and Br?nsted acid,which are responsible for the excellent hydrodeoxygenation *** Ni promotes simultaneously C-O and C-C bonds cleavage to produce pentadecane and hexadecane owing to the increase of electron-rich Mo sites after Ni *** findings contribute to the understanding of the nature of Ni-doped Mo_(2)C on the roles as catalytic active sites for C-O and C-C bonds cleavage.

关键词： Ni-doped Mo_(2)C palmitic acid Hydrodeoxygenation C-O/C-C bond cleavages Biofuel

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Propionate promotes gluconeogenesis by regulating mechanistic target of rapamycin(mTOR)pathway in calf hepatocytes

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Animal Nutrition 2023年第4期15卷 88-98页

作者： Guo Yan Wang Sen Lin Qin Yi Ning Zheng Hui Jun Geng Lei Chen Jun Hu Yao Lu Deng College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi 712100China

Enhancing hepatic gluconeogenesis is one of the main modes of meeting the glucose requirement of dairy *** study attempted to determine whether the gluconeogenesis precursor propionate had an effect on the expression of the main genes involved in gluconeogenesis in calf hepatocytes and elucidate the associated *** hepatocytes were obtained from 5 healthy calves(1 d old;30to 40 kg)and exposed to 0-,1-,2.5-,or 5-mM sodium propionate(NaP),which is known to promote the expression of genes involved in the gluconeogenesis pathway,including fructose 1,6-bisphosphatase,phosphoenolpyruvate carboxykinase,and *** regard to the underlying mechanism,propionate promoted the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha,hepatocyte nuclear factor 4,and forkhead box O1(transcription factors that regulate the expression of hepatic gluconeogenic genes)by promoting mammalian target of rapamycin complex 1(mTORC1),but inhibiting mTORC2 activity(Ppalmitic acid(PA)-induced hepatic injury in calf hepatocytes and found that PA could inhibit the gluconeogenic capacity of calf hepatocytes by suppressing the expression of gluconeogenic genes,inhibiting m TORC1,and promoting the activity of m TORC2(P<0.01).In contrast,NaP provided protection to calf hepatocytes by counteracting the inhibitory effect of PA on the gluconeogenic capacity of calf hepatocytes(P<0.05).Collectively,these findings indicate that NaP enhances the gluconeogenic capacity of calf hepatocytes by regulating the mTOR pathway ***,in addition to improving the glucose production potential,propionate may have therapeutic potential for the treatment of hepatic injury in dairy cows.

关键词： Propionate Gluconeogenesis Mechanistic target of rapamycin palmitic acid

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