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The pael-r gene does not mediate the changes in rotenone-induced Parkinson's disease model cells

Neural regeneration research 2014年第4期9卷 402-406页

作者： Ting Zou Xiangqi Tang Zhiling Huang Niangui Xu Zhiping Hu Department of Neurology Second Xiangya HospitalCentral South University

In this study, we established cell models for Parkinson＇s disease using rotenone. An rNA interfer- ence vector targeting Parkin-associated endothelin receptor-like receptor （Pad-r） was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that pael-r expression was decreased after rNA interference compared with the control group （no treatment） and the model group （rotenone treatment）, while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an MTT assay. These experimental findings indicate that the pael-r gene has no role in the changes in rotenone-induced Parkinson＇s disease model cells.

关键词： nerve regeneration neurodegeneration Parkinson's disease rotenone pael-r rNA interference apoptosis mitochondria neural regeneration

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大鼠pael-r基因shrNA干扰载体的构建及功能筛选鉴定

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生物技术 2011年第4期21卷 12-16页

作者：邹婷肖波汤建光张贺中南大学湘雅二医院神经内科湖南长沙410011 中南大学湘雅医院神经内科湖南长沙410008

目的:构建并筛选针对大鼠pael-r基因的有效shrNA干扰载体并鉴定其干扰效果。方法:构建三个针对大鼠pael-r基因的shrNA表达载体,利用脂质体转染大鼠肾上腺嗜铬细胞瘤细胞PC12,以G418筛选抗性细胞克隆并利用rT-PCr和Western Blotting鉴定P... 详细信息

目的:构建并筛选针对大鼠pael-r基因的有效shrNA干扰载体并鉴定其干扰效果。方法:构建三个针对大鼠pael-r基因的shrNA表达载体,利用脂质体转染大鼠肾上腺嗜铬细胞瘤细胞PC12,以G418筛选抗性细胞克隆并利用rT-PCr和Western Blotting鉴定pael-r基因的表达。结果:在构建的3个干扰载体中,转染prNA-U6/paelr-3的细胞克隆中pael-r表达在mrNA水平为29%,与对照组相比下降了45.3%,在蛋白质水平为32%,下降了27.3%(Ppael-r基因的有效干扰载体prNA-U6/paelr-3,并得到了pael-r基因表达下调型PC12细胞克隆,为研究pael-r基因表达下调在帕金森病的发病机制及其治疗中的作用奠定了基础。

关键词： rNA干扰 pael-r 帕金森病

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Ecto-GPr37:a potential biomarker for Parkinson’s disease

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Translational Neurodegeneration 2021年第1期10卷 77-90页

作者： Xavier Morato Paula Garcia-Esparcia Josep Argerich Franc Llorens Inga Zerr Wojciech Paslawski Eva Borras Eduard Sabido Ulla EPetaja-repo Victor Fernandez-Duenas Isidro Ferrer Per Svenningsson Francisco Ciruela Pharm acology Unit Department of Pathology and Experimental TherapeuticsFaculty of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaUHospitalet de LlobregatSpain Neuroscience Program Bellvitge Biomedical Research InstituteIDIBELLL'Hospitalet de LlobregatSpain Section of Neurology Department of Clinical NeuroscienceDepartm ent of NeuroscienceKarolinska InstituteStockholmSweden Neuropathology Unit Departm ent of Pathology and Experimental TherapeuticsFaculty of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaL'Hospitalet de LlobregatSpain CIBErNED Centro de Investigacion Biomedica en Red de Enfermedades NeurodegenerativasInstituto CarlosⅢMadridSpain Department of Neurology Clinical Dementia Center and National Reference Center for CID SurveillanceUniversity Medical SchoolGottingenGermany German Center for Neurodegenerative Diseases GottingenGermany Proteomics Unit Center for Genomic RegulationBarcelonaSpain Proteomics Unit Universitat Pompeu FabraBarcelonaSpain research Unit of Biomedicine Medical Research Center OuluUniversity of OuluOuluFinland

Objective:a-Synuclein has been studied as a potential biomarker for Parkinson's disease(PD)with no concluding ***,there is an urgent need to find out reliable specific biomarkers for ***37 is an orphan G protein-coupled receptor that toxically accumulates in autosomal recessive juvenile ***,we investigated whether GPr37 is upregulated in sporadic PD,and thus a suitable potential biomarker for ***:GPr37 protein density and mrNA expression in postmortem substantia nigra(SN)from PD patients were analysed by immunoblot and rT-qPCr,*** presence of peptides from the N-terminus-cleaved domain of GPr37(***-GPr37)in human cerebrospinal fluid(CSF)was determined by liquid chromatography-mass spectrometric *** engineered in-house nanoluciferase-based immunoassay was used to quantify ecto-GPr37 in CSF samples from neurological control(NC)subjects,PD patients and Alzheimer's disease(AD)***:GPr37 protein density and mrNA expression were significantly augmented in sporadic *** amounts of ecto-GPr37 peptides in the CSF samples from PD patients were identified by mass spectrometry and quantified by the in-house ELISA ***,the CSF total a-synuclein level in PD patients did not differ from that in NC ***,the cortical GPr37 mrNA expression and CSF ecto-GPr37 levels in AD patients were also ***:GPr37 expression is increased in SN of sporadic PD *** ecto-GPr37 peptides are significantly increased in the CSF of PD patients,but not in AD *** results open perspectives and encourage further clinical studies to confirm the validity and utility of ecto-GPr37 as a potential PD biomarker.

关键词： GPr37 pael-r Parkinson's disease Cerebrospinal fluid Biomarker Alzheimer's disease a-5ynuclein Orphan receptor

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