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Preparation and Evaluation in vitro of Salicylic Acid-pachyman Nanoparticles

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Journal of Wuhan University of Technology(Materials Science) 2011年第4期26卷 606-610页

作者：张军波梁淑彩 School of Pharmaceutical Science Wuhan University

pachyman based nanoparticles loading salicylic acid as model drug （SA-PNPs） were prepared by an inverse microemulsion crosslinking approach using epichlorohydrin （ECH） as crosslinker. The effects of crosslinking reaction time, initial volume ratio of oil to aqueous phase and dosage of crosslinker on the particle size of SA-PNPs were optimized by orthogonal experimental design. SA-PNPs prepared under the optimal conditions had the average size of 230 nm and high encapsulation efficiency of 90%. The in vitro drug release was also investigated and the release data were analyzed using zero order, first order and Higuchi＇s kinetics model. According to the determined coefficients, release data fitted to Higuchi＇s model, which suggested that the release of SA from SA-PNPs in phosphate buffer （pH 7.4） was diffusion controlled release. The experimental results indicated that pachyman possesses a promising potential to be applied as nanocarriers for controlled drug release.

关键词： nanoparticles pachyman salicylic acid

Effects of pachyman in combination with vinorelbine and cisplatin on tumor growth and the expression of EGFR and K-ras in mouse model of lung cancer

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Effects of pachyman in combination with vinorelbine and cisp...

Effects of pachyman in Combination with Vinorelbine and Cisplatin on Tumor Growth and the Expression of EGFR and K-ras in Mice with Lung Cancer

第15届中国中西医结合学会基础理论专业委员会学术年会暨第二届广东省中西医结合学会转化医学专业委员会年会

作者： Jia-ming TIAN Yong-chao CHEN Ling LI Yi NING Jue HU Tao Xiong Fang-guo Lu Ke Wei School of Medicine Hunan University of Chinese Medicine

Objectives: To examine the effects of pachyman in combination with vinorelbine and cisplatin on tumor growth and the expression of epidermal growth factor receptor(EGFR) and K-ras in a mouse model of lung cancer induced, and to investigate the molecular mechanisms underlying the antitumor effects of pachyman. Methods: The size of the tumor xenografts in mice was recorded after treatment with pachyman monotherapy or with pachyman in combination with vinorelbine and cisplatin. We performed immunohistochemical analysis to determine the levels of expression and distribution of EGFR and K-ras in lung cancer tissues, and RT-PCR was used to determine the relative mRNA expression levels. Results: Vinorelbine and cisplatin significantly decreased the rate of growth of A549 xenografts, and pachyman increased the efficacy of vinorelbine and cisplatin. Vinorelbine and cisplatin can significantly decrease the expression, distribution, and mRNA expression levels of EGFR and K-ras in tumor tissues. pachyman monotherapy significantly decreased the distribution and the mRNA expression levels of EGFR in lung cancer tissues. In addition, pachyman in combination with vinorelbine and cisplatin markedly decreased the distribution and expression levels of EGFR in lung cancer tissues. However, pachyman monotherapy or combination therapy did not significantly decrease the mRNA expression levels of K-ras. Conclusions: pachyman in combination with vinorelbine can significantly inhibit the growth of A549 xenografts, and pachyman can regulate the expression of the EGFR gene to increase the efficacy of vinorelbine and cisplatin in lung cancer and decrease the side effects associated with chemotherapy.

关键词： pachyman Lung cancer EGFR K-ras

来源： cnki会议评论

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Digital Chinese Medicine 2018年第4期1卷 310-315页

作者： Ke WEI Jia-Hao ZHOU Yong-Chao CHEN Jia-Ming TIAN Meng-Jin SUN Xin-Wen DAI Bai-Hui LI Ling LI Fang-Liang ZHOU Yi NING Jue HU Qin XIANG Bo ZHANG Fang-Guo LU School of Medicine Hunan University of Chinese MedicineChangshaHunan 410208China Hunan Busky Pharmaceutical Co. LtdChangshaHunan 410005China

Objective To examine the effects of pachyman in combination with vinorelbine and cisplatin on tumor growth and the expression of epidermal growth factor receptor(EGFR)and K-ras in a mouse model of lung cancer induced using the human lung cancer cell line A549,and to investigate the molecular mechanisms underlying the antitumor effects of *** We recorded the size of the tumor xenografts in mice after treatment with pachyman monotherapy or pachymanin combination with vinorelbine and *** performed immunohistochemical analysis to determine the levels of expression and distribution of EGFR and K-ras in lung cancer ***-time fluorescence quantitative PCR was used to determine the relative mRNA expression levels of EGFR and K-ras in lung cancer *** Vinorelbine and cisplatin significantly decreased the rate of growth of A549 xenografts,and pachyman increased the efficacy of vinorelbine and *** and K-ras were widely expressed in A549 *** and cisplatin could significantly decrease the expression,distribution and mRNA expression levels of EGFR and K-ras in tumor *** monotherapy significantly decreased the distribution and the mRNA expression levels of EGFR in lung cancer *** addition,pachyman in combination with vinorelbine and cisplatin markedly decreased the distribution and expression levels of EGFR in lung cancer ***,pachyman monotherapy or combination therapy did not significantly decrease the mRNA expression levels of *** Thus,pachyman in combination with vinorelbine can significantly inhibit the growth of A549 xenografts,and pachyman can regulate the expression of the EGFR gene to increase the efficacy of vinorelbine and cisplatin in lung cancer and decrease the side effects associated with chemotherapy.

关键词： pachyman Vinorelbine Cisplatin Lung cancer EGFR K-ras

Optimal Ultrasonic Extraction of pachyman from Jiuzihe Poria cocos

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Agricultural Science & Technology 2016年第12期17卷 2746-2750,2776页

作者：刘丽徐春李可姜昆吴鹏黄冈师范学院生命科学学院经济林木种质改良与资源综合利用湖北省重点实验室大别山特色资源开发湖北省协同创新中心湖北黄冈438000

This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.

关键词： Poria cocos pachyman Ultrasonic extraction Response surface An-timicrobial activity