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Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules

Science China(Life Sciences) 2017年第8期60卷 852-860页

作者： Xuan Du Sheng Wang Feng Gao Lisha Zhang Jian-Hua Zhao Hui-Shan Guo Chenlei Hua State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences College of Life Sciences University of the Chinese Academy of Sciences College of Agriculture Shihezi University and Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization

Verticillium wilt disease becomes a major threat to many economically important crops. It is unclear whether and how plant immunity takes place during cotton-Verticillium interaction due to the lack of marker genes. Taking advantage of cotton （Gossypium hirsutum） genome, we discovered pathogenesis-related （pr） gene families, which have been widely used as markers of immune responses in plants. To profile the expression of G. hirsutum pr genes in the process of plant immunity, we treated cotton roots with two immunogenic peptides, fig22 and nlp20 known as pathogen-associated molecular patterns, as well as three Verticillium dahliae-derived peptides, nlp20vd2, nlp23vd3, and nlp23vd4 which are highly identical to nlp20. Quantitative real-time PCR results revealed that 14 G. hirsutum pr gene （Ghpr） families were induced or suppressed independently in response to fig22, nip20, nlp20va2, nlp23vd3, and nlp23vd4. Most Ghpr genes are expressed highest at 3 h post incubation of immunogenic peptides. Compared to fig22 and nlp20, nlp20vd2 is more effective to trigger up-regulated expression of Ghpr genes. Notably, both nlp23vd3 and nlp23vd4 are able to induce Ghpr gene up-regulation, although they do not induce necrosis on cotton leaves. Thus, our results provide marker genes and new immunogenic peptides for further investigation of cotton-V, dahliae interaction.

关键词： Verticillium wilt Gossypium hirsutum pr gene PAMP nip20

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The Wound Induced AP2/ERF Domain Transcription Factor WRERF50 Confers Resistance to Necrotrophic Fungi,Independent of Salicylate,Ethylene and Jasmonate Signaling Pathways in Arabidopsis

The Wound Induced AP2/ERF Domain Transcription Factor WRERF5...

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中国植物病理学会2012年学术年会

作者： WANG Cheng-Gang~1 HUANG Jun-Yan~1 ZHOU Rong LIU Sheng-Yi~* (Oil Crops Research Institute of CAAS,Key Lab of Oil Crops Biology of the Ministry of Agriculture, Wuhan,430062,China)

＜正＞Necrotrophic pathogens are an agriculturally important group of destructive pathogens. One of them is Sclerotinia sclerotiorum,which attacks more than 400 plant species and is one of the most important diseases in oil crops in the *** is also the major diseases of oilseed rape in *** little is known about molecular mechanisms of host resistance to ***,which thus limited development of resistance improvement *** identified WRERF50 gene from cDNA microarray of *** inoculated with *** clarify regulation of WRERF50 gene expression in response to *** infection,wild-type Arabidopsis were treated with wounding and plant hormone ethephon,MeJA and SA and then WRERF50 expression of different treatment and different time points were analyzed by quantitative RT-PCR;in addition,we also detected WRERF50 expression in three mutant backgrounds（npr1-1,coil-1 and ein2-1 ）.The results suggested that expression of WRERF50 was induced by wounding,independent of ET,JA,and SA signaling ***, Over-expression of WRERF50 activates expression of several pr genes PDF1.2,ChiB and pr-2,increased plant resistance against necrotrophic fungi Botrys cinerea and Sclerotinia sclerotiorum while WRERF50-silencing plants down-regulated expression of several pr genes and decreased resistance to both pathogens.

关键词： Transcription factor WRERF50 S.sclerotiorum Plant hormone pr gene

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