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Journal of Integrative Agriculture 2023年第4期22卷 972-980页

作者： CHEN Chang-zhao WANG Ya-liang HE Meng-xing LI Zhi-wen SHEN Lan LI Qing REN Deyong HU Jiang ZHU Li ZHANG Guang-heng GAO Zhen-yu ZENG Da-li GUO Long-biao QIAN Qian ZHANG Qiang State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004P.R.China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 310006P.R.China

Photosynthesis occurs mainly in chloroplasts,whose development is regulated by proteins encoded by nuclear *** them,pentapeptide repeat(ppr)proteins participate in organelle RNA *** there are more than 450 members of the ppr protein family in rice,only a few affect RNA editing in rice *** editing technology has created new rice germplasm and mutants,which could be used for rice breeding and gene function *** study evaluated the functions of Osppr9 in chloroplast RNA editing in *** osppr9 mutants were obtained by CRISPR/Cas9,which showed yellowing leaves and a lethal phenotype,with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related *** addition,loss of Osppr9 protein function reduces the editing efficiency of rps8-C182,rpoC2-C4106,rps14-C80,and ndhB-C611 RNA editing sites,which affects chloroplast growth and development in *** data showed that Osppr9 is highly expressed in rice leaves and encodes a DYW-ppr protein localized in ***,the Osppr9 protein was shown to interact with OsMORF2 and ***,our findings provide insights into the role of the ppr protein in regulating chloroplast development in rice.

关键词： rice(Oryza sativa L.) ppr protein chloroplast development RNA editing

GhCTSF1,a short ppr protein with a conserved role in chloroplast development and photosynthesis,participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton

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Plant Communications 2024年第6期5卷 213-229页

作者： Yuzhu Huo Mengxue Cheng Meiju Tang Meng Zhang Xiaofan Yang Yating Zheng Tong Zhao Peng He Jianing Yu College of Life Sciences Shaanxi Normal UniversityXi’an 710119China

Cotton is one of the most important textile fibers *** crucial agronomic traits,leaves play an essential role in the growth,disease resistance,fiber quality,and yield of cotton *** repeat(ppr)proteins are a large family of nuclear-encoded proteins involved in organellar or nuclear RNA *** a virus-induced gene silencing assay,we found that cotton plants displayed variegated yellow leaf phenotypes with decreased chlorophyll content when expression of the ppr gene GhCTSF1 was ***1 encodes a chloroplast-localized protein that contains only two ppr *** of GhCTSF1 substantially reduces the splicing efficiency of rpoC1 intron 1 and ycf3 intron *** of function of the GhCTSF1 ortholog EMB1417 causes splicing defects in rpoC1 and ycf3-2,leading to impaired chloroplast structure and decreased photosynthetic rates in *** also found that GhCTSF1 interacts with two splicing factors,GhCRS2 and *** in GhCRS2 and GhWTF1 severely affect intron splicing of rpoC1 and ycf3-2 in cotton,leading to defects in chloroplast development and a reduction in *** results suggest that GhCTSF1 is specifically required for splicing rpoC1 and ycf3-2 in cooperation with GhCRS2 and GhWTF1.

关键词： chloroplast RNA splicing ppr protein cotton photosynthesis

Young Leaf White Stripe encodes a P-type ppr protein required for chloroplast development

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Journal of Integrative Plant Biology 2023年第7期65卷 1687-1702页

作者： Jie Lan Qibing Lin Chunlei Zhou Xi Liu Rong Miao Tengfei Ma Yaping Chen Changling Mou Ruonan Jing Miao Feng Thanhliem Nguyen Yulong Ren Zhijun Cheng Xin Zhang Shijia Liu Ling Jiang Jianmin Wan State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Pentatricopeptide repeat(ppr) proteins function in post-transcriptional regulation of organellar gene expression. Although several ppr proteins are known to function in chloroplast development in rice(Oryza sativa), the detailed molecular functions of many ppr proteins remain ***, we characterized a rice young leaf white stripe(ylws) mutant, which has defective chloroplast development during early seedling ***-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted ppr protein with 11 ppr motifs. Further expression analyses showed that many nuclear-and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2,and rps12, and editing of ndhA, ndhB, and rps14transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development.

关键词： chloroplast development ppr protein ribosome biogenesis RNA splicing

Studies on a ppr protein YELLOW SEEDLING 1 in regulation of chloroplast development at the early growth stage in Arabidopsis

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Studies on a PPR protein YELLOW SEEDLING 1 in regulation of ...

纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Wenbin Zhou and Jirong Huang Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032

＜正＞Plastid transcription is performed by nuclear-encoded RNA polymerases （NEP） and the plastid-encoded RNA polymerase （PEP）. PEP-dependent gene expression is crucial for chloroplast development and is temporall... 详细信息

关键词： Arabidopsis ppr protein regulation of chloroplast development

A rice novel gene ALBL encoding a ppr protein functions in plastid gene translation

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A rice novel gene ALBL encoding a PPR protein functions in p...

2016年全国植物生物学大会

作者： Yaozong Wu Qinghua Li Hualing Mi National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

Plastid gene translation is carried out in its ribosome which RNAs undergo posttranscriptional processing. Several pentatricopeptide repeat(ppr) proteins are involved in the processes. However,the mechanism underlying the regulatory mRNA processing for plastid ribosome genes remains to be elucidated. Here,we report that a rice ppr protein ALBL plays crucial role in plastid gene expression by participating in the maturation of the precursor of plastid 23S-4.5S rRNA. We isolated ALBL gene from a rice(Oryza sativa) mutant,nearly albino phenotype(albl) by map-based cloning method,and identified ALBL as a novel nucleus-encoded ppr protein with a C-terminal DYW domain,and localized to the chloroplast. Mutation of ALBL resulted in the dramatic decrease of photosynthetic proteins and abnormal chloroplasts in the seedling,plastid ribosome deficiency. albl was defected the maturation of 23S-4.5S r RNA,while significantly increased in the amount of its precursor transcripts. Based on these results,we propose that ALBL is required for plastid gene expression,participating in the maturation process of 23S-4.5S r RNA.

关键词： ppr protein r RNA processing plastid rRNA chloroplast

Cloning and Analysis Expression Patterns of Novel ppr Gene in Cotton

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Cloning and Analysis Expression Patterns of Novel PPR Gene i...

中国棉花学会2013年年会

作者： HE Peng LIU Xue-mei YU Jia-ning College of Life Sciences Shaanxi Normal University

ppr ( pentatricopeptide repeats protein ) gene family was widely found in terrestrial plants , and they were involved in plant development , organelle biogenesis , restoring of cytoplasmic male sterilities , post-translational processing and modification , cell signaling , and responses to environmental *** , the ppr have been found in many eukaryotic genes , but little was researched in *** this study , we cloned a novel pprgene GhpprH1in Gossypium hirsutum , which encoded 637amino acid *** tissue expression pattern of this gene indicated that GhpprH1was high expression in roots , leaves and 15-day fibers , Cluster analysis showed that GhpprH1shared closed relationship with a ppr in Prunus persica.

关键词： Gossypium hirsutum ppr protein expression patterns

A Novel Chloroplast-Localized Pentatricopeptide Repeat protein Involved in Splicing Affects Chloroplast Development and Abiotic Stress Response in Rice

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Molecular Plant 2014年第8期7卷 1329-1349页

作者： Junjie Tan Zhenhua Tan Fuqing Wu Peike Sheng Yueqin Heng Xinhua Wang Yulong Ren Jiulin Wang Xiuping Guo Xin Zhang Zhijun Cheng Ling Jiang Xuanming Liu Haiyang Wang Jianmin Wan State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology Hunan University Changsha 410082 P.R. China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of AgriculturalSciences Beijing 100081 RR. China National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R. China

Pentatricopeptide repeat （ppr） proteins comprise a large family in higher plants and modulate organellar gene expression by participating in various aspects of organellar RNA metabolism. In rice, the family contains 477 members, and the majority of their functions remain unclear. In this study, we isolated and characterized a rice mutant, white stripe leaf （wsl）, which displays chlorotic striations early in development. Map-based cloning revealed that WSL encodes a newly identified rice ppr protein which targets the chloroplasts. In wsl mutants, PEP-dependent plastid gene expression was significantly down-regulated, and plastid rRNAs and translation products accumulate to very low levels. Consistently with the observations, wsl shows a strong defect in the splicing of chloroplast transcript rpl2, resulting in aberrant transcript accumulation and its product reduction in the mutant. The wsl shows enhanced sensitivity to ABA, salinity, and sugar, and it accumulates more H2O2 than wild-type. These results suggest the reduced translation efficiency may affect the response of the mutant to abiotic stress.

关键词： ppr protein WSL ABA chloroplast RNA splicing rpl2 abiotic stress responses.

MEF13 Requires MORF3 and MORF8 for RNA Editing at Eight Targets in Mitochondrial mRNAs in Arabidopsis thaliana

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Molecular Plant 2015年第10期8卷 1466-1477页

作者： Franziska Glass Barbara Hartel Anja Zehrmann Daniil Verbitskiy Mizuki Takenaka Molekulare Botanik Universitat UIm 89069 UIm Germany These authors contributed equally to this article.

RNA editing sites in plant mitochondria and plastids are addressed by pentatricopeptide repeat （ppr） proteins with E or E and DYW domains, which recognize a specific nucleotide motif upstream of the edited nucleotide. In addition, some sites require MORF proteins for efficient RNA editing. Here, we assign the novel E domain-containing ppr protein, MEF13, as being required for editing at eight sites in Arabidopsis thaliana. A SNP in ecotype C24 altering the editing level at only one of the eight target sites was located by genomic mapping. An EMS mutant allele of the gene for MEF13 was identified in a SNaPshot screen of a mutated plant population. At all eight target sites of MEF13, editing levels are reduced in both morf3 and morf8 mutants, but at only one site in morfl mutants, suggesting that specific MEF13-MORF interactions are required. Yeast two-hybrid analyses detect solid connections of MEF13 with MORF1 and weak contact with MORF3 proteins. Yeast three-hybrid （Y3H） analysis shows that the presence of MORF8 enhances the connection between MEF13 and MORF3, suggesting that a MORF3-MORF8 heteromer may form stably or transiently to establish interaction with MEF13.

关键词： RNA editing plant mitochondria ppr protein MEF13 MEF-MORF interaction

Fine mapping of cisc(t),a gene for cold-induced seedling chlorosis,and identification of its candidate in rice

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Science Bulletin 2010年第Z2期 3149-3153页

作者： LAN Tao 1,2,WANG Bin 1,LING QiuPing 1,XU ChunHua 1,TONG ZhiJun 1,LIANG KangJing 1,2,DUAN YuanLin 1,2,JIN Jing 1 &WU WeiRen 1,2 1Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fuzhou 350002,China 2 Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding,Fuzhou 350002,China

The seedlings of indica rice cultivar Dular are susceptible to chlorosis under low temperature *** previous studies indicated that low temperature-induced seedling chlorosis is controlled by a recessive gene,located between SSR markers RM257 and RM242,on the long arm of chromosome *** temporarily named the gene cisc(t).Using a large F2 population derived from a cross between Dular and the japonica cultivar Lemont,which displays a normal green color at low temperatures,cisc(t)was fine mapped to within a 12-kb *** is only one annotated gene in this interval,which encodes a pentatricopeptide repeat(ppr)*** analysis indicated that 8 bases were deleted at the 60th base in the Dular allele,resulting in a frame-shift mutation and loss of function of the *** is consistent with the chlorosis mutant phenotype of *** addition,previous studies have shown that many chlorosis mutants of seedlings are related to ppr ***,we presume that the ppr gene is the candidate for cisc(t).

关键词： rice seedling low temperature albino fine mapping ppr protein

The Pentatricopeptide Repeat protein SOT5/EMB2279 is Required for Plastid rpl2 and trnK Intron Splicing

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The Pentatricopeptide Repeat Protein SOT5/EMB2279 is Require...

第七届长三角植物科学研讨会暨青年学术报告会

作者： Weihua Huang Yajuan Zhu Wenjuan Wu Xuan Li Delin Zhang Ping Yin Jirong Huang Department of Biology College of Life and Environmental SciencesShanghai Normal University Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences National Key Laboratory of Crop Genetic Improvement National Centre of Plant Gene ResearchHuazhong Agricultural University

Chloroplast biogenesis and development are highly complex processes requiring interaction between plastid and nuclear genomic *** a high-throughput screen for chloroplast biogenesis suppressors in Arabidopsis thaliana,we identified a suppressor of thf1(sot5) that displays virescent and serrated *** characterization revealed that sot5 mutants are defective in leaf adaxial and abaxial polarity,and act as enhancer of asymmetric ***-based cloning identified SOT5 as a gene previously named EMB2279 that encodes a plastid-targeted pentatricopeptide repeat(ppr) protein with 11 ppr motifs.A G-to-A mutation in sot5 leads to a significant decrease in splicing efficiency,generating two additional mRNA *** previously reported,the sot5 null mutation is embryo ***5 is predicted to bind to specific RNA sequences found in plastid rpl2 and trnK genes,and we found decreased splicing efficiency of the rpl2 and trnK genes in sot5 ***,our results reveal that the ppr protein SOT5/EMB2279 is required for intron splicing of plastid rpl2 and trnK,providing insights into the role of plastid translation in the coupled development between chloroplasts and leaves.

关键词： Arabidopsis ppr protein plastid gene intron splicing