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The dynamic interactions between chemotherapy drugs and plasmid dna investigated by atomic force microscopy

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Science China Materials 2017年第3期60卷 269-278页

作者：李密刘连庆肖秀斌席宁王越超 State Key Laboratory of Robotics Shenyang Institute of Automation Chinese Academy of Sciences Department of Lymphoma Affiliated Hospital of Military Medical Academy of Sciences Department of Electrical and Computer Engineering Michigan State University

The advent of atomic force microscopy （AFM） provides a powerful tool for imaging individual dna molecules. Chemotherapy drugs are often related to dnas. Though many specific drug-dna interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid dnas is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid dnas and two commercial chemotherapy drugs （methotrexate and cisplatin）. plasmid dnas were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid dnas after the stimulation of methotrexate and cisplatin. Geometric features of plasmid dnas were extracted from AFM images and the statistical results showed that the geometric features of plasmid dnas changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid dnas at the single-molecule level.

关键词： atomic force microscopy plasmid dna drug methotrexate cisplatin

Amplification of plasmid dna bound on soil colloidal particles and clay minerals by the polymerase chain reaction

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Journal of Environmental Sciences 2007年第11期19卷 1326-1329页

作者： CAI Peng HUANG Qiao-yun LU Yan-du CHEN Wen-li JIANG Dai-hua LIANG Wei State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070 China Key Laboratory of Subtropical Agricultural Resources a~ld Environment Huazhong Agricultural University Wuhan 430070 China

Polymerase chain reaction （PCR） was used to amplify a 600-base pair （bp） sequence of plasmid pGEX-2T dna bound on soil colloidal particles from Brown soil （Alfisol） and Red soil （Ultisol）, and three different minerals （goethite, kaolinite, montmorillonite）. dna bound on soil colloids, kaolinite, and montmorillonite was not amplified when the complexes were used directly but amplification occurred when the soil colloid or kaolinite-dna complex was diluted, 10- and 20-fold. The montmorillonite-dna complex required at least 100-fold dilution before amplification could be detected. dna bound on goethite was amplified irrespective of whether the complex was used directly, or diluted 10- and 20-fold. The amplification of mineral-bound plasmid dna by PCR is, therefore, markedly influenced by the type and concentration of minerals used. This information is of fundamental importance to soil molecular microbial ecology with particular reference to monitoring the fate of genetically engineered microorganisms and their recombinant dna in soil environments.

关键词： adsorption amplification mineral PCR plasmid dna soil colloid

Functionalization of Single- and Multi-Walled Carbon Nanotubes with Cationic Amphiphiles for plasmid dna Complexation and Transfection

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Nano Research 2009年第8期2卷 638-647页

作者： Cyrille Richard Nathalie Mignet Céline Largeau Virginie Escriou Michel Bessodes Daniel Scherman Unitéde Pharmacologie Chimique et Génétique CNRSUMR 8151ParisF-75270 cedex France INSERM U 640ParisF-75270 cedex France UniversitéParis Descartes Facultédes Sciences Pharmaceutiques et BiologiquesParisF-75270 cedex France ENSCP ParisF-75231 cedex France

The possibility of delivering dna effi ciently to cells represents a crucial issue for the treatment of both genetic and acquired ***,even although the effi ciency of non-viral transfection systems has improved in the last decade,none have yet proven to be suffi ciently effective in *** report herein our results on the functionalization of single-walled carbon nanotubes(SWNT)and multi-walled carbon nanotubes(MWNT)by two cationic amphiphiles(lipid RPR120535 and pyrenyl polyamine),their use for the complexation of plasmid dna,and their efficiency in transfecting cells in *** experiments have shown that the efficiency of transfection is higher when using SWNT instead of MWNT,and that transfection effi ciency is similar or slightly higher when using nanoplexes(SWNT/lipid RPR120535/dna)instead of lipoplexes(lipid RPR120535/dna)and several orders of magnitude higher than that of naked *** study therefore shows both that the transfection is better when using SWNTs and that it is dependent on the nature of the amphiphilic molecules adsorbed on the nanotubes.

关键词： Carbon nanotubes amphiphile gene delivery plasmid dna in vitro

Effects of medium composition on the production of plasmid dna vector potentially for human gene therapy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2005年第5期6卷 396-400页

作者：徐志南沈文和陈灏岑沛霖 Institute of Bioengineering School of Material Science and Chemical Engineering Zhejiang University Hangzhou 310027 China

plasmid vector is increasingly applied to gene therapy or gene vaccine. The production of plasmid pCMV-AP3 for cancer gene therapy was conducted in a modified MBL medium using a recombinant E. coli BL21 system. The effects of different MMBL components on plasmid yield, cell mass and specific plasmid dna productivity were evaluated on shake-flask scale. The results showed that glucose was the optimal carbon source. High plasmid yield (58.3 mg/L) was obtained when 5.0 g/L glucose was added to MMBL. Glycerol could be chosen as a complementary carbon source because of the highest specific plasmid pro- ductivity (37.9 mg dna/g DCW). After tests of different levels of nitrogen source and inorganic phosphate, a modified MMBL medium was formulated for optimal plasmid production. Further study showed that the initial acetate addition (less than 4.0 g/L) in MMBL improved plasmid production significantly, although it inhibited cell growth. The results will be useful for large-scale plasmid production using recombinant E. coli system.

关键词： plasmid dna Growth medium Gene therapy

Catalytic hydrolysis of phosphate diester (BNPP) and plasmid dna by mononuclear macrocyclic polyamine metal complexes

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Chinese Chemical Letters 2009年第5期20卷 523-526页

作者： Qing Xiang Xiang Li Qun Zhang Xiao Qi Yu Ru Gang Xie Department of Chemistry and Life Science Leshan Teachers College Leshan 614004 China Department of Chemistry Sichuan University Chengdu 610064 China

The activities of the catalytic hydrolysis of phosphate diester （BNPP） [bis（p-nitrophenyl）phosphate diester] and plasmid dna （pUC 18） by mononuclear macrocyclic polyamine metal complexes have been investigated in this paper. The results showed that the highest activity in hydrolysis of BNPP was obtained with le--Zn（II） complex （composed of lipophilic group） as catalyst. The hydrolysis rate enhancement is up to 3.64 × 10^4 fold. These metal complexes could effectively promote the cleavage of plasmid dna （pUC18） at physiological conditions.

关键词： Catalytic hydrolysis Macrocyclic polyamine Zn(II) complex BNPP plasmid dna

Optimization on cationic liposome-mediated cell transfection of plasmid dna

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The Chinese-German Journal of Clinical Oncology 2011年第5期10卷 290-292页

作者： Mingang Ying Changhua Zhuo Weidong Zang Department of Abdominal Surgery Fujian Provincial Cancer Hospital Teaching Hospital of Fujian Medical University Fuzhou 350014 China

Objective:The development of gene carriers for efficient gene delivery into cells has attracted growing attention in recent *** aim of this study was to achieve a better outcome of AAV-293 cells transfection by plasmid ***:We studied the optimal condition for higher efficiency of cationic lipid-mediated cell *** experimental groups were *** dna and liposome were mixed in each groups at different ratios(μg:μL),1:2.5,1:3.5,1:4.0 and 1:5.0,*** gene functioned as reporter gene,measuring the transfection efficiency of the four groups using the method of X-gal ***:When the ratio was 1:3.5,the cell transfection rate was the *** the ratio of 1:2.5 recommended by product manual achieve the lowest transfection *** difference had statistical ***:In order to obtain a higher transfection efficiency,optimization on conditions of the ratio of plasmid dna to liposome is necessary in cell transfection.

关键词： cell transfection cationic lipid plasmid dna cell culture transfection efficiency

STM OF GLYCOPHORIN A, F-ACTIN AND plasmid dna IN WATER

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北京大学学报(医学版) 1993年第1期25卷 77-79+84页

作者：董群戴建武苏雅娴商广义贺节姚骏恩北京医科大学北京中科院北京电子显微镜实验室北京电子显微镜开放实验室北京

The surface structure of three uncoated biological macromolecules in water wasstudied by STM, namely glyciphorin A (GPA) from human erythrocyte membranes, F-actinfrom macrophages in abdominal cavity of Kun-ming mouse ... 详细信息

关键词： Scanning tunneling microscope Glycophorin A F-actin plasmid dna

Delivery of plasmid dna into Tumors by Intravenous Injection of PEGylated Cationic Lipoplexes into Tumor-Bearing Mice

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Pharmacology & Pharmacy 2016年第7期7卷 272-282页

作者： Yoshiyuki Hattori Department of Drug Delivery Research Hoshi University Tokyo Japan

For systemic injection of cationic liposome/plasmid dna (pdna) complexes (cationic lipoplexes), polyethylene glycol (PEG)-modification (PEGylation) of lipoplexes can enhance their systemic stability. In this study, we examined whether intravenous injection of PEGylated cationic lipoplexes into tumor-bearing mice could deliver pdna into tumor tissues and induce transgene expression. PEGylation of cationic liposomes could prevent their agglutination with erythrocytes. However, when PEGylated cationic lipoplexes were injected intravenously into tumor-bearing mice, they accumulated in tumor vascular vessels and did not exhibit transgene expression in tumors with both poor and well-developed vascularization. Furthermore, PEGylated cationic lipoplexes of CpG- free pdna could not increase transgene expression in tumors after intravenous injection. These results suggested that PEGylation could not extravasate cationic lipoplexes from vascular vessels in tumors and abolished transgene expression although it enhanced the systemic stability of cationic lipoplexes by avoiding interactions with blood components such as erythrocytes. Successful delivery of pdna to tumors by PEGylated cationic liposomes will require a rational strategy and the design of liposomal delivery systems to overcome the issue associated with the use of PEG.

关键词： Cationic Liposome Lipoplex plasmid dna PEGylation Tumor

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

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SIGNAL TRANSDUCTION AND TARGETED THERAPY 2024年第1期9卷 1-66页

作者： Lu, Peilin Ruan, Dongxue Huang, Meiqi Tian, Mi Zhu, Kangshun Gan, Ziqi Xiao, Zecong Sun Yat Sen Univ Affiliated Hosp 3 Nanomed Res Ctr Guangzhou 510630 Peoples R China Guangzhou Med Univ Affiliated Hosp 2 Dept Minimally Invas Intervent Radiol Guangzhou 510260 Peoples R China Guangzhou Med Univ Affiliated Hosp 2 Lab Intervent Radiol Guangzhou 510260 Peoples R China Guangzhou Med Univ Affiliated Hosp 1 Guangzhou Inst Resp Hlth State Key Lab Resp DisNatl Clin Res Ctr Resp Dis Guangzhou 510120 Peoples R China Chengdu Second Peoples Hosp Dept Stomatol Chengdu 610021 Peoples R China Sun Yat Sen Univ Hosp Stomatol Guanghua Sch Stomatol Guangdong Prov Key Lab Stomatol Guangzhou 510055 Peoples R China

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements and the potential of hydrogels in therapeutic applications, focusing primarily on two areas: emerging cell-based therapies and promising non-cell therapeutic modalities. Within the context of cell therapy, we discuss the capacity of hydrogels to overcome the existing translational challenges faced by mainstream cell therapy paradigms, provide a detailed discussion on the advantages and principal design considerations of hydrogels for boosting the efficacy of cell therapy, as well as list specific examples of their applications in different disease scenarios. We then explore the potential of hydrogels in drug delivery, physical intervention therapies, and other non-cell therapeutic areas (e.g., bioadhesives, artificial tissues, and biosensors), emphasizing their utility beyond mere delivery vehicles. Additionally, we complement our discussion on the latest progress and challenges in the clinical application of hydrogels and outline future research directions, particularly in terms of integration with advanced biomanufacturing technologies. This review aims to present a comprehensive view and critical insights into the design and selection of hydrogels for both cell therapy and non-cell therapies, tailored to meet the therapeutic requirements of diverse diseases and situations.

关键词： MESENCHYMAL STEM-CELLS GELATIN-METHACRYLOYL HYDROGELS CROSS-LINKABLE HYDROGELS DRUG-DELIVERY SYSTEM T-CELL GROWTH-FACTOR MECHANICAL-PROPERTIES PHOTODYNAMIC THERAPY INJECTABLE HYDROGEL plasmid dna