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Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

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Chinese Medical Journal 2018年第2期131卷 218-225页

作者： Hamed Shoorei Arash Khaki Nava Ainehchi Mohammad Mehdi Hassanzadeh Taheri Moloud Tahmasebi Giti Seyedghiasi Ziba Ghoreishi Majid Shokoohi Amir Afshin Khaki Sayed Haidar Abbas Raza Department of Anatomical Sciences Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran Department of Pathology Tabriz Branch Islamic Azad University Tabriz Iran Women's Rep oduciive Heaiih Rese'arch Center Ta'briz University of Medical Sciences Tabriz Iran Department of Anatomical Sciences Faculty of Medicine Birjand University of Medical Sciences Birjand Iran Department of Anatomical Sciences Faculty of Medicine Tarbiat Modares University Tehran Iran Department of Nursing Faculty of Paramedical Mashhad University of Medical Sciences Mashhad Iran Department of Biology College of Animal Science and Technology Northwest A&F University Yangling Shaanxi 712100 China

Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods： Isolated preantral follicles were randomly divided into three main groups： the control group containing 10% fetal bovine serum without MC extract （G1）, the first experimental group supplemented with 25 ktg/ml hydroalcoholic extract of chamomile （G2）, and the second experimental group supplemented with 50 p,g/ml hydroalcoholic extract of chamomile （G3）. Results： After 12 days of culture, the survival rate （P 〈 0.05）, antrum formation （P 〈 0.01）, metaphase two oocytes （P 〈 0.01）, and the expression ofpcna （P 〈 0.05） and FSHR （P 〈 0.05） genes significantly decreased in G3 as compared with GI. On the other hand, at the last day of culture （day 12）, the mean diameter of follicles cultured in the medium which was supplemented with 50 lag/ml hydroalcoholic extract of chamomile significantly decreased as compared with the GI （P 〈 0.05）. In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G I （P 〈 0.01）, while in the medium of G 1, the level of 17[3-estradiol was significantly higher than that of other groups （P 〈 0.01）. Reactive oxygen species levels of metaphase 11 oocytes were significantly decreased in G2 as compared with G1 （P 〈 0.01）. Conclusion： Adding chamomile extract to culture media appeared to decrease follicular function and development.

关键词： Follicular Development Follicular Maturation FSHR gene pcna gene Steroid Hormones Toxicology

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