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Frontiers of Medicine 2021年第5期15卷 750-766页

作者： Manling Liu Zhaoling Shi Yue Yin Yishi Wang Nan Mu Chen Li Heng Ma Qiong Wang Department of physiology and pathophysiology Fourth Military Medical UniversityXi’an 710032China Department of pediatrics Second Affiliated Hospital of Shaanxi University of Chinese MedicineXianyang 712046China Department of Cardiovascular Medicine Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Exposure to particulate matter 2.5(pM2.5)potentially triggers airway inflammation by activating nuclear factor-κB(NF-κB).Sirtuin 2(SIRT2)is a key modulator in ***,the function and specific mechanisms of SIRT2 in pM2.5-induced airway inflammation are largely ***,this work investigated the mechanisms of SIRT2 in regulating the phosphorylation and acetylation of p65 influenced by pM2.5-induced airway inflammation and bronchial *** revealed that pM2.5 exposure lowered the expression and activity of SIRT2 in bronchial ***,SIRT2 impairment promoted the phosphorylation and acetylation of p65 and activated the NF-κB signaling *** activation of p65 triggered airway inflammation,increment of mucus secretion by goblet cells,and acceleration of tracheal ***,p65 phosphorylation and acetylation,airway inflammation,and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice exposed to ***(a specific p65 inhibitor)reversed p65 activation and ameliorated pM2.5-induced airway inflammation and bronchial *** findings provide novel insights into the molecular mechanisms underlying the toxicity of pM2.5 *** inhibition of p65 phosphorylation and acetylation could be an effective therapeutic approach in averting pM2.5-induced airway inflammation and bronchial hyperresponsiveness.

关键词： particulate matter 2.5 sirtuin 2 p65 airway inflammation bronchial hyperresponsiveness triptolide

piperine treating sciatica through regulating inflammation and MiR-520a/p65 pathway

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Chinese Journal of Natural Medicines 2021年第6期19卷 412-421页

作者： YU Jiu-Wang LI Sha BAO Li-Dao WANG Lu Department of pharmacy Affiliated Hospital of Inner Mongolia Medical UniversityHohhot 010059China Mongolia Medical School Inner Mongolia Medical UniversityHohhot 010110China

Although the etiology of sciatica remains uncertain,there is increasing evidence that the disease process of sciatica is associated with the levels of inflammatory ***,an alkaloid isolated from piper nigrum,has previously been demonstrated to inhibit inflammation and analgesic *** purpose of this study is to verify the regulatory relationship between miR-520a and p65 and to explore how miR-520a/p65 affects the level of cytokines under the action of piperine,so as to play a therapeutic role in *** ELISA experiment,we confirmed that four inflammatory factors(IL-1β,TNF-α,IL-10,TGF-β1)can be used as evaluation indexes of *** differentially expressed miRNA was screened as miR-520a,by microarray technology,and the downstream target of miR-520a was p65 by ***-time fluorescence quantitative pCR confirmed that the expression of miR-520a was negatively correlated with pro-inflammatory cytokines,positively correlated with anti-inflammatory cytokines and negatively correlated with p65 expression at mRNA *** expression of p65 was positively correlated with pro-inflammatory cytokines and negatively correlated with anti-inflammatory cytokines at the protein level verified by ELISA and Western *** staining analysis showed that the nerve fibers were repaired by piprine,the vacuoles were significantly reduced,and the degree of nerve fiber damage was also *** analysis showed that the expression of p65 decreased after administration of ***-luciferase reporter gene assay confirmed that the luciferase signal decreased significantly after cotransfection of miR-520a mimics and p653'UTR recombinant *** sum up,in the rat model of non-compressed lumbar disc herniation,piperine plays a significant role in ***-520a can specifically and directly target p65,and piperine can promote the expression of miR-520a,then inhibit the expression of p65,down-regulate the pro-inflammatory

关键词： piperine Sciatica miR-520a p65 Inflammatory factor

SUMOylation and deacetylation affect NF-κB p65 activity induced by high glucose in human lens epithelial cells

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International Journal of Ophthalmology(English edition) 2019年第9期12卷 1371-1379页

作者： Xiao Han Xiao-Xuan Dong Ming-Yu Shi Li Feng Xin-Ling Wang Jin-Song Zhang Qi-Chang Yan Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityKey Laboratory of Lens Research of Liaoning ProvinceEye Hospital of China Medical UniversityShenyang110005Liaoning ProvinceChina Department of Ophthalmology the Fourth People’s Hospital of ShenyangShenyang110031Liaoning ProvinceChina

AIM: To explore the effects of IκBα SUMOylation and NF-κB p65 deacetylation on NF-κB p65 activity induced by high glucose in cultured human lens epithelial cells(HLECs).METHODS: HLECs(SRA01/04) were cultured with 5.5, 25, and 50 mmol/L glucose media for 24 h, and with 50 mmol/L glucose media for 0, 12, and 24 h respectively. SUMO1 and SIRT1 expressions were detected by reverse transcriptionpolymerase chain reaction(RT-pCR) and Western blot(WB). IκBα and NF-κB p65 expressions were detected by WB. With NAC, DTT, MG132 or Resveratrol(RSV) treatment, SUMO1 and SIRT1 expressions were detected by WB. protein expression localizations were examined by immunofluorescence and co-immunofluorescence. The effects of SUMO1 or SIRT1 overexpression, as well as MG132 and RSV, on the nuclear expression and activity of IκBα and NF-κB p65 were analyzed by immunoblot and dual luciferase reporter gene ***: SUMO1 and SIRT1 expressions were influenced by high glucose in mRNA and protein levels, which could be blocked by NAC or DTT. SUMO1 was down-regulated by using MG132, and SIRT1 was up-regulated under RSV treatment. IκBα nuclear expression was attenuated and NF-κB p65 was opposite under high glucose, while IκBα and NF-κB p65 location was transferred to the nucleus. SUMO1 or SIRT1 overexpression and MG132 or RSV treatment affected the nuclear expression and activity of IκBα and NF-κB p65 under high glucose ***: IκBα SUMOylation and NF-κB p65 deacetylation affect NF-κB p65 activity in cultured HLECs under high glucose, and presumably play a significant role in controlling diabetic cataract.

关键词： SUMOylation deacetylation NF-κB p65 IκBα diabetic cataract high glucose lens epithelial cells

Effect of atorvastatin on expression of TLR4 and NF-κB p65 in atherosclerotic rabbits

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Asian pacific Journal of Tropical Medicine 2013年第6期6卷 493-496页

作者： Ding-Zhu Shen San-Li Xin Chuan Chen Te Liu Shanghai Geriatric Institute of Chinese Medicine Longhua Hospital Affiliated Shanghai University of Traditional Chinese Medicine

Objective:To study the effect of atorvastatin on atherosclerotic ***:A total of 60 Now Zealand male rabbits were randomly divided into the normal group,model group and atorvastatin *** replication rabbit atherosclerotic model with immune injury combined with a high fat diet feeding was *** rabbits were sacrificed after 3 ***4 and NF-κB p65 were observed by HE staining,immunohistochemistry and western ***: The expression of TLR4,NF- k B p65 were significantly increased in the model group compared widi the normal *** expression of TI.R4 and NK- k B p65 decreased significantly in the atorvastatin group,and there was no difference compared with the normal ***: The effect of atorvastatin on adierosclerosis may be achieved by the inhibition of the expression of TLK4 and NF-κB p65.

关键词： Atherosclerosis Atorvastatin TLR4 NF-κB p65

Effect of casein glycomacropeptide on subunit p65 of nuclear transcription factor-B in lipopolysaccharide-stimulated human colorectal tumor HT-29 cells

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Food Science and Human Wellness 2014年第2期3卷 51-55页

作者： Jianmiao Gong Qingsen Chen Yali Yan Guangchang pang Tianjin Key Laboratory of Food Biotechnology College of Biotechnology and Food ScienceTianjin University of CommerceTianjin 300134China

Objective:The effect of casein glycomacropeptide(CGMp)on the expression of NF-B subunit p65 in human colorectal cancer HT-29 cells induced by lipopolysaccharide(LpS)was investigated to explore the therapeutic efficacy of CGMp for human colorectal ***:HT-29 cells cultured in 96-well plates were stimulated with LpS for 30 min at the concentrations of 0,0.001,0.01,0.1,1 and 10g/*** stimulation,the expression of p65 in HT-29 cells was evaluated by immunofluorescence ***,HT-29 cells were incubated with 0,0.001,0.01,0.1,1,10 and 100g/mL CGMp for 24 h at the optimal LpS *** treatment with CGMp under the incubation with optimal LpS concentration for 30 min,the protein expression of p65 was analyzed by Western *** optimum culture time was evaluated by incubating HT-29 cells with the optimal CGMp concentration for 6,12,24,48 and 72 ***:NF-B-p65 revealed that the highest protein expression was achieved with 1g/mL LpS ***,CGMp could inhibit the protein expression of NF-B-p65 in LpS-stimulated HT-29 cells,and the optimal inhibitory effect was observed at a CGMp concentration of 0.01g/mL with 48 h ***:CGMp can regulate NF-B signaling pathway through inhibiting the expression of its subunit p65,which is beneficial for the further improvement of human colorectal cancer treatment.

关键词： colorectal stimulation p65

JNK3通过与RelA/p65相互作用抑制NF-κB信号通路减弱Bel-7402细胞的黏附能力(英文)

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生物化学与生物物理进展 2012年第9期39卷 877-886页

作者：李强韩卿虞东辉唐留军王建王晓辉许望翔詹轶群李长燕葛常辉于淼杨晓明北京放射医学研究所北京100850 蛋白质组学国家重点实验室北京100850

JNK信号通路在细胞的炎症、增殖与凋亡等生物学过程中发挥了重要的作用.我们采用酵母双杂交技术发现转录因子p65是JNK3的相互作用蛋白质.体内体外实验均证实JNK3与p65存在蛋白质相互作用.报告基因实验结果表明过表达JNK3抑制TNFα诱导NF... 详细信息

JNK信号通路在细胞的炎症、增殖与凋亡等生物学过程中发挥了重要的作用.我们采用酵母双杂交技术发现转录因子p65是JNK3的相互作用蛋白质.体内体外实验均证实JNK3与p65存在蛋白质相互作用.报告基因实验结果表明过表达JNK3抑制TNFα诱导NF-κB介导的转录激活.EMSA结果证明JNK3减弱NF-κB的DNA结合能力.实时定量pCR结果表明JNF3减少NF-κB靶基因的表达.综上所述,我们的研究结果表明JNK3做为一个调节分子在体内发挥了抑制p65转录活性的功能.

关键词： JNK3 NF-κB p65 细胞黏附蛋白质相互作用

在人乳腺癌细胞增殖中LXRα、NF-κB p65和cyclinD1的差异表达（英文）

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生物化学与生物物理进展 2016年第3期43卷 226-235页

作者：涂剑刘晓旺李涛余平丁维珂陆凯强陈霄霄彭露周志刚南华大学药物药理研究所衡阳421001 南华大学第一附属医院衡阳421001

LXRα可以抑制包括乳腺癌在内的多种肿瘤细胞的增殖,而这种抑制作用的具体机制尚不明了.因此本文探讨了LXRα、NF-κB p65和cyclin D1三者在人乳腺癌细胞增殖中的差异表达.首先采用免疫组化法检测了乳腺癌及癌旁组织中LXRα,NF-κB p65... 详细信息

LXRα可以抑制包括乳腺癌在内的多种肿瘤细胞的增殖,而这种抑制作用的具体机制尚不明了.因此本文探讨了LXRα、NF-κB p65和cyclin D1三者在人乳腺癌细胞增殖中的差异表达.首先采用免疫组化法检测了乳腺癌及癌旁组织中LXRα,NF-κB p65和cyclin D1的表达,结果表明,3种蛋白主要表达于细胞核,且NF-κB p65和cyclin D1在癌组织中的表达显著高于癌旁组织,而LXRα则在癌组织中表达显著降低.用MTT和Western blot检测TO901317对乳腺癌细胞增殖及3种蛋白表达的影响,结果显示,随着TO901317浓度的增加与时间的延长,其对细胞增殖的抑制作用逐渐增强,与此同时能上调LXRα的表达,并下调NF-κB p65和cyclin D1的表达.进一步通过RNA干扰技术下调LXRα的表达,以及应用NF-κB抑制剂pDTC观察TO901317上述作用的改变,发现LXRαsiR NA显著降低TO901317对3种蛋白表达的影响和对细胞增殖的抑制作用.而pDTC则加强TO901317的上述作用效果,除了几乎不影响LXRα的表达外.综上所述,LXRα/NF-κB p65/cyclin D1在乳腺癌细胞增殖中具有重要作用,有助于进一步明确LXRα为新的乳腺癌调控靶点,为今后乳腺癌的分子靶向治疗提供新的思路.

关键词：肝X受体α 核因子-κB p65 周期蛋白D1 细胞增殖 TO901317

Nuclear factor-kB p65 (RelA) transcription factor is constitutively activated in human colorectal carcinoma tissue

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World Journal of Gastroenterology 2004年第22期10卷 3255-3260页

作者： Liang-LiangYu Hong-GangYu Jie-pingYu He-ShengLuo Xi-MingXu Jun-HuaLi DepartmentofGastroenterology RenminHospitalWuhanUniversityWuhan430060HubeiProvinceChina LaboratoryforExperimentalGastroenterology DepartmentofMedicineISt.JosephHospitalRuhr-UniversityBochumBochumGermany

AIM: Activation of transcription factor nuclear factor-κB(NF-κB) has been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. The purpose of this study was to determine whether NF-κB was constitutively activated in human colorectal tumor tissues and, if so, to determine the role of NF-κB in colorectal tumorigenesis, and furthermore, to determine the association of RelA expression with tumor cell apoptosis and the expression of Bcl-2 and Bcl-xL. METHODS: paraffin sections of normal epithelial, adenomatous and adenocarcinoma tissues were analysed immunohistochemically for expression of RelA, Bcl-2 and Bcl-xL proteins. Electrophoretic mobility shift assay (EMSA) was used to confirm the increased nuclear translocation of RelA in colorectal tumor tissues. The mRNA expressions of Bcl-2 and Bcl-xL were determined by reverse transcription polymerase chain reaction (RT-pCR) analysis. Apoptotic cells were detected triphosphate fluorescence nick end labeling (TUNEL) method. RESULTS: The activity of NF-κB was significantly higher in adenocardnoma tissue in comparison with that in adenomatous and normal epithelial tissues. The apoptotic index (AI) significantly decreased in the transition from adenoma to adenocarcinoma. Meanwhile, the expressions of Bcl-2 and Bcl-xL protein and their mRNAs were significantly higher in adenocarcinoma tissues than that in adenomatous and normal epithelial tissues. CONCLUSION: NF-κB may inhibit apoptosis via enhancing the expression of the apoptosis genes Bcl-2 and Bcl-xL. And the increased expression of RelA/nuclear factor-κB plays an important role in the pathogenesis of colorectal carcinoma.

关键词：原子核基因-κB p65 RelA 转录因子血液活性结直肠癌肿瘤 NF-κB

补阳还五汤对动脉粥样硬化模型大鼠主动脉Rho激酶及NF-κB p65 mRNA表达的影响

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中国中西医结合杂志 2015年第12期35卷 1495-1500页

作者：张红珍李丽焦瑞张英钱艳山西中医学院中西医结合临床学院太原030619 山西医科大学第二医院中医科太原030001 山西医科大学计算机教学部太原030001 山西医科大学公共卫生学院太原030001

目的观察益气活血法代表方剂补阳还五汤对动脉粥样硬化(atherosclerosis,AS)模型大鼠主动脉组织Rho激酶及核转录因子-κB(nuclear transcription factor kappa B,NF-κB)p65 mRNA表达及血脂指标的影响。方法采用维生素D3联合高脂饮食法... 详细信息

目的观察益气活血法代表方剂补阳还五汤对动脉粥样硬化(atherosclerosis,AS)模型大鼠主动脉组织Rho激酶及核转录因子-κB(nuclear transcription factor kappa B,NF-κB)p65 mRNA表达及血脂指标的影响。方法采用维生素D3联合高脂饮食法制备大鼠AS模型。将60只大鼠随机分为正常对照组、模型组、补阳还五汤低剂量组(10 g/kg)、补阳还五汤高剂量组(20 g/kg)、辛伐他汀组(0.6 mg/kg)及补阳还五汤预防组(10 g/kg)。造模成功后给药干预28天,采用ELISA法检测氧化型低密度脂蛋白(ox-LDL)水平,酶法测定血清TG、TC、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)水平。光镜下观察主动脉组织AS病理变化。采用实时荧光定量pCR法检测主动脉Rho激酶及NF-κB p65 mRNA表达水平。结果高脂饮食加维生素D3腹腔注射可诱导大鼠形成AS模型,模型组大鼠主动脉有典型的粥样斑块形成。与正常对照组比较,模型组TC、TG及LDL-C及ox-LDL水平明显升高,HDL-C下降(ppppp65 mRNA表达水平明显升高(pp65 mRNA表达水平明显降低(ppp65 mRNA表达水平比较,差异无统计学意义(p>0.05)。结论补阳还五汤可下调主动脉Rho激酶及NF-κB p65mRNA表达水平,降低血脂,具有抗AS作用,抑制Rho激酶通路可能是其作用机制之一。

关键词：益气活血法补阳还五汤动脉粥样硬化 Rho激酶 NF-κB p65

NF-κB p65,p38 MApK在慢性间歇性缺氧大鼠肺损伤中的表达及意义

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中南大学学报（医学版） 2015年第12期40卷 1313-1319页

作者：葛丽荞明婷侯瑾闫静王强乔峰西安医学院第一附属医院耳鼻咽喉头颈外科西安710077 西安医学院第一附属医院麻醉科西安710077 西安交通大学第二附属医院耳鼻咽喉头颈外科西安710004 西安医学院第一附属医院感染控制科西安710077

目的:探讨反复缺氧条件下,核因子κB(NF-κB)、p38丝裂原活化蛋白激酶(p38 MApK)在慢性间歇性缺氧大鼠肺组织损伤过程中的作用。方法:40只雌性SD大鼠,随机分为对照组和缺氧组,每组20只。缺氧组大鼠每天在自动控制间歇性缺氧试验箱饲养8... 详细信息

目的:探讨反复缺氧条件下,核因子κB(NF-κB)、p38丝裂原活化蛋白激酶(p38 MApK)在慢性间歇性缺氧大鼠肺组织损伤过程中的作用。方法:40只雌性SD大鼠,随机分为对照组和缺氧组,每组20只。缺氧组大鼠每天在自动控制间歇性缺氧试验箱饲养8 h;对照组正常喂养、不加处理。所有大鼠饲养35 d后,取肺组织行HE染色、免疫组织化学测定NF-κB p65和p38 MApK的表达;Western印迹检测肺组织NF-κB p65的表达。结果:缺氧组大鼠肺组织的病理改变明显;缺氧组肺泡间隔厚度(2.15±0.49)μm,对照组肺泡间隔厚度(0.45±0.12)μm,其差异具有统计学意义(pp65及p38 MApK棕褐色阳性染色表达均明显增强,与对照组相比差异有统计学意义(pp65蛋白水平较对照组明显增加,其差异具有统计学意义(pp65及p38 MApK信号通路可能在间歇缺氧大鼠肺组织重构的发生发展过程中发挥重要作用。

关键词：慢性间歇低氧肺损伤 NF-κB p65 p38丝裂原活化蛋白激酶