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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：梁尚栋刘双梅吴炳彭力超宋渺渺邹丽芳吕秋兰张熙南昌大学医学院生理教研室

Objective p2x3 receptors in stellate ganglia（SG）and cervical dorsal root ganglia（DRG）neurons are involved in sympathoexcitatory reflex induced by myocardial ischemic ***,a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen,has been widely used in treatment of myocardial and cerebral *** present study is aimed to observe the effects of puerarin on the signaling transmission mediated by p2x3 receptor in SG and DRG after myocardial ischemic *** Systolic blood pressure,heart rate were measured by the indirect tail-cuff plethysmography（non-invasive blood pressure determinator）with different *** expression levels of p2x3 m RNA and protein in SG and DRG were tested by RT-pCR and Western *** electrophysiological recording was carried out by using a patch/whole cell clamp *** Our results showed that systolic blood pressure and heart rate increased,and the expression levels of p2x3m RNA and protein in SG and DRG were up-regulated after myocardial ischemic *** reduced systolic blood pressure and heart rate,relieved pain and decreased up-regulated expression of p2x3 m RNA and protein in SG and DRG after myocardial *** inhibited the up-regulated ATp-activated currents in DRG neurons after myocardial *** also significantly inhibited ATp-activated currents in HEK293 cells transfected with p2x3 *** puerarin can relieve myocardial ischemic damage through blocking the p2x3 signaling transmission and then depressed the aggravated sympathoexcitatory reflex.

关键词： p2x3 receptor stellate ganglia dorsal root ganglia puerarin myocardial ischemia

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Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第3期18卷 239-248页

作者： xiao-fen HE Jun-jun WEI Sheng-yun SHOU Jian-qiao FANG Yong-liang JIANG Department of Neurobiology and Acupuncture Research the Third Clinical Medical College Zhejiang Chinese Medical University Hangzhou 310053 China Zhejiang Chinese Medical University Hangzhou 310053 China

Objective： To investigate the analgesic effects of electroacupuncture （EA） at 2 and 100 Hz on type 2 diabetic neuropathic pain （DNp） and on the expressions of the p2x3 receptor and calcitonin gene-related peptide （CGRp） in the dorsal root ganglion （DRG）. Methods： Rat type 2 DNp was induced by a high calorie and high sugar diet fed for 7 weeks, plus a single intraperitoneal injection of streptozotocin （STZ） after 5 weeks. EA at 2 and 100 Hz was carried out once every day after 7 weeks for 7 consecutive days. Body weight, serum fasting insulin （FINS）, fasting blood glucose （FBG）, insulin sensitivity index （ISI）, and paw withdrawal latency （pWL） were measured. The expressions of L4-L6 DRG p2x3 receptors and CGRp were assessed by immunofluorescence. Results： Type 2 DNp was successfully induced as shown by the increased body weight, FINS, and FBG, as well as the reduced ISI and pWL. Expressions of p2x3 receptors and CGRp in L4-L6 DRGs increased. EA at both 2 and 100 Hz relieved type 2 DNp, but the analgesic effect of EA was stronger at 2 Hz. p2x3 receptor expression decreased in L4-L6 DRGs following EA at 2 Hz and in L5 and L6 DRGs following EA at 100 Hz. EA at both 2 and 100 Hz down-regulated CGRp overexpression in L4-L6 DRGs. Conclusions： These findings indicate that EA at 2 Hz is a good option for the management of type 2 DNp. The EA effect may be related to its down-regulation of the overexpressions of the DRG p2x3 receptors and CGRp in this condition.

关键词： Electroacupuncture Type 2 diabetic neuropathic pain Dorsal root ganglion p2x3 receptor Calcitoningene-related peptide

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peripheral sensitization:roles and mechanisms for cancer-induced bone pain

Peripheral sensitization:roles and mechanisms for cancer-ind...

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中国神经科学学会第十届全国学术会议

作者： Qin ZHENG Min LIU Dong FANG Jie CAI You WAN Ji-Sheng HAN Guo-Gang xING Neuroscience Research Institute Peking University Department of Neurobiology School of Basic Medical SciencesPeking University Health Science Center

Objective Bone cancer pain resulting from primary tumors or tumors that metastasize to bone is one of the most severe and intractable types of cancer pain,which reduces the quality of life of *** mechanisms underlying the development of cancer-induced bone pain remain largely *** studies have now demonstrated that hyperexcitation of nociceptive sensory neurons,*** sensitization,plays an important role in the pathogenesis of neuropathic pain and inflammatory *** present study is to explore the role of peripheral sensitization in cancer-induced bone *** Using whole-cell patch clamp recording,together with techniques of molecular biology,cell biology,immunohistochemistry,and behavioral pharmacology,we first investigated changes in excitabilities of nociceptive dorsal root ganglion(DRG) neurons in cancer rats and the association with bone cancer *** we examined the role of Kv7(KCNQ/M) potassium channels and p2x3 receptors in regulation of DRG neurons excitability and their contribution to cancer-induced bone ***(1) Implantation of tumor cells into the tibial canal in rats produced hyperexcitation of nociceptive DRG neurons that likely contributed to the peripheral sensitization and cancer-induced bone pain.(2) Suppression of Kv7(KCNQ/M) channels in primary DRG neurons plays crucial roles in hyperexcitation of these DRG neurons and the development of bone cancer pain.(3) Visinin-like protein 1(VTLIp-1),a member of neuronal calcium sensor proteins,which was first detected in DRG neurons in our experiments,interacted with p2x3 receptor C-terminus and enhanced the expression and function of the receptor,which in turn caused hyperexcitation of DRG neurons that is proposed to underlie the pathogenesis of cancer-induced bone *** Suppression of Kv7(KCNQ/M) channels and functional upregulation of p2x3 receptors by VILIp-1in DRG neurons contributes to the development of peripheral sensitization and cancer-i

关键词： bone cancer pain peripheral sensitization Kv7(KCNQ/M potassium channel p2x3 receptor neuronal calcium sensor protein VILIp-1

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