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Electrophoresis(SDS-pAGE)as a Method for Screening Species of passiflora Using Seed proteins as Molecular Markers

Journal of Agricultural Science and Technology(B) 2022年第2期12卷 40-47页

作者： Marilia penteado Stephan Ana Maria Costa Tatiana De Lima Azevedo Jeane Santos Da Rosa Alexsandro Araújo Dos Santos Embrapa Food Technology Laboratory of Biochemistry Avenida das AméricasRio de Janeiro 29501RJ 23020470Brazil Embrapa Cerrados BR-020km 18Mailbox:08223Planaltina 73310-970DFBrazil

Brazil is the center of diversity and dispersion of species of the genus *** species of passion fruit passiflora tenuifila BRS VT(passiflora garlic)and another of passiflora setacea BRS pC were used in this *** plants were grown on the Embrapa Cerrados experimental *** fruits were washed,minimally cut and their constituents *** species differed in the proportion of wet mass between husk,seed,and *** expected,both species had a higher proportion of husks and seeds and a lower amount of pulp.A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis(SDS-pAGE).The quantification of soluble proteins was performed using the Bradford *** analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of *** and 28.5 times for ***,in the *** was verified that both species present a similar protein profile,observed by the intense and diverse bands found in the polyacrylamide gel,mainly in the range of 32 to 19 *** work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive *** objective of this work was to present an analytical procedure to semi-purify seed proteins of two passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them.

关键词： protein screening method bioactive peptides seeds p.setacea p.tenuifila mass distribution on fruit

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