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phosphoproteomic identification and phylogenetic analysis of ribosomal p-proteins in populus dormant terminal buds

Phosphoproteomic identification and phylogenetic analysis of...

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第二届中国林业学术大会——S2 功能基因组时代的林木遗传与改良

作者： Chang-Cai Liu1)a Tian-Cong Lu 1)a Hua-Hua Li3) Gui-Feng Liu5) Bai-Chen Wang8)b (Education Ministry Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University Harbin 150040)Hong-Xia Wang4) (Institute of Basic Medical Sciences, National Center for Biomedical Analysis Beijing 100850) Ling Ma6) (Forestry school, Northeast Forestry University Harbin 150040) Chuan-ping Yang7)b (Education Ministry Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University Harbin 150040 Forestry school, Northeast Forestry University Harbin 150040)

To better understand the role that reversible phosphorylation plays in woody plant ribosomal p-protein function, we initiated a phosphoproteomic investigation of p-proteins from populus dormant terminal buds. Using gel-free (in-solution) protein digestion and phosphopeptide enrichment combined with a nanoUpLC-ESI-MS/MS strategy, we identified six phosphorylation sites on eight p-proteins from populus dormant terminal buds. Among these, six Ser sites and one Thr site were identified in the highly conserved C-terminal region of eight p-proteins of various p-protein subfamilies, including two p0, two p1, three p2, and one p3 protein. Among these, the Thr site was shown to be novel and has not been identified in any other organisms. Sequence analysis indicated that the phosphothreonine sites identified in the C-terminus of ptr Rpp2A exclusively occurred in woody species of populus etc. The identified phosphopeptides shared a common phosphorylation motif of (S/T)XX(D/E) and may be phosphorylated in vivo by casein kinase 2 as suggested by using Scansite analysis. Furthermore, phylogenetic analysis suggested that divergence of p2 also occurred in populus, including Type I and Type II. To the best of our knowledge, this is the first systematic phosphoproteomic and phylogenetic analysis of p-proteins in woody plants, the results of which will provide a wealth of resources for future understanding and unraveling of the regulatory mechanisms of populus p-protein phosphorylation during the maintenance of dormancy.

关键词： Dormant terminal buds NanoUpLC-ESI-MS/MS phosphoproteomics phylogenetic analysis p-proteins

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