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Vitamins C, E, and NADH on in Vitro Lymphocyte Proliferation and Redox status among Obese Patients

Food and Nutrition Sciences 2016年第12期7卷 1082-1098页

作者： Asmaa Meraou Hafida Merzouk Amel Saidi Amel Medjdoub Sid Ahmed Merzouk Slimane Belbraouet Laboratory of Physiology Physiopathology and Biochemistry of Nutrition Department of Biology Faculty of Natural and Life Sciences Earth and Universe University Abou-Bekr Belkaid of Tlemcen Tlemcen Algeria University Djilali liabes of Sidi Bel Abbes Sidi Bel Abbes Algeria Department of Technical Sciences Faculty of Engineering University Abou-Bekr Belkaid of Tlemcen Tlemcen Algeria Ecole de Nutrition Université de Moncton Moncton Canada

Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.

关键词： Obesity Lymphocytes Vitamins Cytokines oxidant/antioxidant status

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