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Comparative proteomic study and functional analysis of translationally controlled tumor protein in rice roots under Hg^(2+) stress

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Journal of Environmental Sciences 2012年第12期24卷 2149-2158页

作者： Feijuan Wang Yongshen Shang Ling Yang Cheng Zhu College of Life Sciences Zhejiang UniversityHangzhou 310058China College of Life Sciences China Jiliang UniversityHangzhou 310018China College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004China

So far, very little is known about mercury stress-induced intercellular metabolic changes in rice roots at the proteome level. To investigate the response of rice roots to mercury stress, changes in protein expression in rice roots were analyzed using a comparative proteomics approach. Six-leaf stage rice seedlings were treated with 50 μmol/L HgC12 for 3 hr; 29 protein spots showed a significant changes in abundance under stress when compared with the Hg2＋-tolerant rice mutant and wild type （Zhonghua 11）. Furthermore, all these protein spots were identified by mass spectrometry to match 27 diverse protein species. The identified proteins were involved in several processes, including stress response, redox homeostasis, signal transduction, regulation and metabolism; some were found to be cellular structure proteins and a few were unknown. Among the up-regulated proteins, ostctp （translationally controlled tumor protein） was chosen to perform hetereologous expression in yeast which was presumed to participate in the Hg2~ tolerance of rice, providing evidence for its role in alleviating Hg2~ damage. Among the many tests, we found that ostctp-overexpressed yeast strains were more resistant to Hg2＋ than wild-type yeast. Thus, we propose that ostctp contributes to Hg2＋ resistance. Here we present, for the first time, the functional characterization of ostctp in connection with Hg2＋ stress in plants.

关键词： 2-DE mercury stress rice roots ostctp heteroexpression

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